{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T11:00:37Z","timestamp":1775473237990,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T00:00:00Z","timestamp":1751155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for Science and Technology","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"Foundation for Science and Technology","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"Foundation for Science and Technology","award":["2022.10505.BD"],"award-info":[{"award-number":["2022.10505.BD"]}]},{"name":"Foundation for Science and Technology","award":["2022.13777.BD"],"award-info":[{"award-number":["2022.13777.BD"]}]},{"name":"Regulamento do Emprego Cient\u00edfico e Tecnol\u00f3gico\u2014RJEC","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"Regulamento do Emprego Cient\u00edfico e Tecnol\u00f3gico\u2014RJEC","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"Regulamento do Emprego Cient\u00edfico e Tecnol\u00f3gico\u2014RJEC","award":["2022.10505.BD"],"award-info":[{"award-number":["2022.10505.BD"]}]},{"name":"Regulamento do Emprego Cient\u00edfico e Tecnol\u00f3gico\u2014RJEC","award":["2022.13777.BD"],"award-info":[{"award-number":["2022.13777.BD"]}]},{"name":"FCT Ph.D.","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"FCT Ph.D.","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"FCT Ph.D.","award":["2022.10505.BD"],"award-info":[{"award-number":["2022.10505.BD"]}]},{"name":"FCT Ph.D.","award":["2022.13777.BD"],"award-info":[{"award-number":["2022.13777.BD"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoX"],"abstract":"<jats:p>The use of cellular energy allocation (CEA) as a physiological energetic biomarker is useful for detecting the sublethal effects of environmental contaminants. The CEA assesses the health and energy status of organisms, serving as a reliable indicator for monitoring the health of aquatic ecosystems. This study aimed to evaluate the impact of emerging contaminants already listed as a priority for monitoring in freshwater ecosystems, namely sulfamethoxazole (0.156\u20132.50 mg\/L), trimethoprim (25.0\u2013400 mg\/L), 4-chloroaniline (5.21\u201320.0 mg\/L), and 3,4-dichloroaniline (0.38\u20134.00 mg\/L), on the CEA of D. rerio embryos. A standard fish embryo toxicity test was conducted, and an adaptation of the allometric scaling approach was developed through the relationship between the size and the fresh weight of the embryos. All the compounds affected the fractions of the energy reserves (total carbohydrate, lipid, and protein contents) differently, with carbohydrates being the predominant energy fraction and the most responsive indicator. Although the energy consumed showed no significant changes, the CEA was notably altered after exposure to all the contaminants, indicating a direct connection to shifts in the available energy. The CEA alterations may indicate a reallocation of energy toward detoxification, combating the stress of contaminant exposure. Energy allocation biomarkers provide a comprehensive assessment of an organism\u2019s physiological state, which is essential for evaluating emerging contaminants\u2019 impacts, safeguarding aquatic ecosystems, and shaping effective environmental policies.<\/jats:p>","DOI":"10.3390\/jox15040099","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T07:55:43Z","timestamp":1751442943000},"page":"99","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Metabolic Costs of Emerging Contaminants: Cellular Energy Allocation in Zebrafish Embryos"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0813-7602","authenticated-orcid":false,"given":"B\u00e1rbara S.","family":"Diogo","sequence":"first","affiliation":[{"name":"Abel Salazar Biomedical Sciences Institute (ICBAS), University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"},{"name":"Centre Interdisciplinary of Marine and Environmental Research, Laboratory Associated (CIIMAR\/CIMAR, LA), University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre S\/N, 4169-007 Porto, Portugal"}]},{"given":"Daniela","family":"Rebelo","sequence":"additional","affiliation":[{"name":"Abel Salazar Biomedical Sciences Institute (ICBAS), University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"},{"name":"Centre Interdisciplinary of Marine and Environmental Research, Laboratory Associated (CIIMAR\/CIMAR, LA), University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre S\/N, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6686-9919","authenticated-orcid":false,"given":"Sara C.","family":"Antunes","sequence":"additional","affiliation":[{"name":"Centre Interdisciplinary of Marine and Environmental Research, Laboratory Associated (CIIMAR\/CIMAR, LA), University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre S\/N, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9093-6464","authenticated-orcid":false,"given":"Sara","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre Interdisciplinary of Marine and Environmental Research, Laboratory Associated (CIIMAR\/CIMAR, LA), University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua do Campo Alegre S\/N, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Morin-Crini, N., Lichtfouse, E., Liu, G., Balaram, V., Ribeiro, A.R.L., Lu, Z., Stock, F., Carmona, E., Teixeira, M.R., and Picos-Corrales, L.A. 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