{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:23:20Z","timestamp":1760235800274,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>Serotonin is an important monoamine in the human body, playing crucial roles, such as a neurotransmitter in the central nervous system. Previously, our group reported that \u03b2-adrenergic drugs (ICI 118,551, isoprenaline, and propranolol) influence the proliferation of breast cancer cells (MCF-7 cells) and their inherent production of adrenaline. Thus, we aimed to investigate the production of serotonin in MCF-7 cells, clarifying if there is a relationship between this production and the viability of the cells. To address this question, briefly, we treated the MCF-7 cells with ICI 118,551, isoprenaline, and propranolol, and evaluated cellular viability and serotonin production by using MTT, Sulforhodamine B (SRB) and Neutral Red (NR) assays, and HPLC-ECD analysis, respectively. Our results demonstrate that isoprenaline promotes the most pronounced endogenous synthesis of serotonin, about 3.5-fold greater than control cells. Propranolol treatment also increased the synthesis of serotonin (when compared to control). On the other hand, treatment with the drug ICI 118,551 promoted a lower endogenous synthesis of serotonin, about 1.1-fold less than what was observed in the control. Together, these results reveal that MCF-7 cells can produce serotonin, and the drugs propranolol, isoprenaline and ICI 118,551 influence this endogenous production. For the first time, after modulation of the \u03b2-adrenergic system, a pronounced cellular growth can be related to higher consumption of serotonin by the cells, resulting in decreased levels of serotonin in cell media, indicative of the importance of serotonin in the growth of MCF-7 cells.<\/jats:p>","DOI":"10.3390\/jpm11100954","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T04:55:33Z","timestamp":1632718533000},"page":"954","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Serotonin after \u03b2-Adrenoreceptors\u2019 Exposition: New Approaches for Personalized Data in Breast Cancer Cells"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8231-1560","authenticated-orcid":false,"given":"Ana Salom\u00e9","family":"Correia","sequence":"first","affiliation":[{"name":"OncoPharma Research Group, Center for Health Technology and Services Research (CINTESIS), Rua Dr. Pl\u00e1cido da Costa, 4200-450 Porto, Portugal"},{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1420-5042","authenticated-orcid":false,"given":"Diana","family":"Duarte","sequence":"additional","affiliation":[{"name":"OncoPharma Research Group, Center for Health Technology and Services Research (CINTESIS), Rua Dr. Pl\u00e1cido da Costa, 4200-450 Porto, Portugal"},{"name":"Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"given":"Isabel","family":"Silva","sequence":"additional","affiliation":[{"name":"Clinical Chemistry, Department of Laboratory Pathology, Centro Hospitalar Universit\u00e1rio do Porto (CHUP), Largo Prof. Abel Salazar, 4099-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0246-6790","authenticated-orcid":false,"given":"Henrique","family":"Reguengo","sequence":"additional","affiliation":[{"name":"Clinical Chemistry, Department of Laboratory Pathology, Centro Hospitalar Universit\u00e1rio do Porto (CHUP), Largo Prof. Abel Salazar, 4099-313 Porto, Portugal"},{"name":"Unit for Multidisciplinary Research in Biomedicine (UMIB), University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2142-6839","authenticated-orcid":false,"given":"Jos\u00e9 Carlos","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Clinical Chemistry, Department of Laboratory Pathology, Centro Hospitalar Universit\u00e1rio do Porto (CHUP), Largo Prof. Abel Salazar, 4099-313 Porto, Portugal"},{"name":"Unit for Multidisciplinary Research in Biomedicine (UMIB), University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1283-1042","authenticated-orcid":false,"given":"Nuno","family":"Vale","sequence":"additional","affiliation":[{"name":"OncoPharma Research Group, Center for Health Technology and Services Research (CINTESIS), Rua Dr. Pl\u00e1cido da Costa, 4200-450 Porto, Portugal"},{"name":"Department of Community Medicine, Information and Health Decision Sciences (MEDCIDS), Faculty of Medicine, University of Porto, Al. Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.biochi.2018.06.016","article-title":"Serotonin and human cancer: A critical view","volume":"161","author":"Sarrouilhe","year":"2019","journal-title":"Biochimie"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.2174\/1566524015666150114113411","article-title":"Serotonin and Cancer: What Is the Link?","volume":"15","author":"Sarrouilhe","year":"2015","journal-title":"Curr. Mol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1742-4682-7-34","article-title":"Serotonin synthesis, release and reuptake in terminals: A mathematical model","volume":"7","author":"Best","year":"2010","journal-title":"Theor. Biol. Med. Model."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Yadav, V.K. (2013). 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