{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T07:13:17Z","timestamp":1775113997487,"version":"3.50.1"},"reference-count":93,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T00:00:00Z","timestamp":1588377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/138325\/2018"],"award-info":[{"award-number":["SFRH\/BD\/138325\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/BIO\/04469\/2020"],"award-info":[{"award-number":["UIDB\/BIO\/04469\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000004"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000004"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["LISBOA-010145-FEDER-022059"],"award-info":[{"award-number":["LISBOA-010145-FEDER-022059"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Polyphenols are plant secondary metabolites with diverse biological and potential therapeutic activities such as antioxidant, anti-inflammatory and anticancer, among others. However, their extraction from the native plants is not enough to satisfy the increasing demand for this type of compounds. The development of microbial cell factories to effectively produce polyphenols may represent the most attractive solution to overcome this limitation and produce high amounts of these bioactive molecules. With the advances in the synthetic biology field, the development of efficient microbial cell factories has become easier, largely due to the development of the molecular biology techniques and by the identification of novel isoenzymes in plants or simpler organisms to construct the heterologous pathways. Furthermore, efforts have been made to make the process more profitable through improvements in the host chassis. In this review, advances in the production of polyphenols by genetically engineered Saccharomyces cerevisiae as well as by synthetic biology and metabolic engineering approaches to improve the production of these compounds at industrial settings are discussed.<\/jats:p>","DOI":"10.3390\/life10050056","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T03:29:39Z","timestamp":1588562979000},"page":"56","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Synthetic Biology Approaches to Engineer Saccharomyces cerevisiae towards the Industrial Production of Valuable Polyphenolic Compounds"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3963-234X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Rainha","sequence":"first","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7703-2391","authenticated-orcid":false,"given":"Daniela","family":"Gomes","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9265-0630","authenticated-orcid":false,"given":"L\u00edgia R.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3217-2320","authenticated-orcid":false,"given":"Joana L.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,2]]},"reference":[{"key":"ref_1","first-page":"293","article-title":"Secondary metabolites of plants and their role: Overview","volume":"9","author":"Pagare","year":"2016","journal-title":"Curr. Trends Biotechnol. Pharm."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yang, L., Wen, K.S., Ruan, X., Zhao, Y.X., Wei, F., and Wang, Q. (2018). Response of plant secondary metabolites to environmental factors. Molecules, 23.","DOI":"10.3390\/molecules23040762"},{"key":"ref_3","unstructured":"Galanakis, C.M. (2018). Polyphenols: Properties, Recovery, and Applications, Woodhead Publishing."},{"key":"ref_4","unstructured":"(2020, April 21). Global Polyphenols Market Will Register a CAGR of Around 9.0% by 2027. Available online: https:\/\/proficientmarket.com\/press-release\/1225\/global-polyphenols-market."},{"key":"ref_5","unstructured":"(2020, April 21). Global Flavonoids Market Will Reach USD 1047.63 Million in 2021. Available online: https:\/\/www.zionmarketresearch.com\/news\/global-flavonoids-market."},{"key":"ref_6","unstructured":"(2020, April 21). Resveratrol Market\u2014Global Industry Trends and Forecast to 2027|Data Bridge Market Research. Available online: https:\/\/www.databridgemarketresearch.com\/reports\/global-resveratrol-market."},{"key":"ref_7","unstructured":"(2020, April 21). Curcumin Market Size Worth $151.9 Million By 2027. Available online: https:\/\/www.grandviewresearch.com\/press-release\/curcumin-market."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"117463","DOI":"10.1016\/j.lfs.2020.117463","article-title":"A review on anti-cancer properties of quercetin in breast cancer","volume":"248","author":"Ezzati","year":"2020","journal-title":"Life Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Tomeh, M.A., Hadianamrei, R., and Zhao, X. (2019). A review of curcumin and its derivatives as anticancer agents. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20051033"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biotechadv.2019.04.007","article-title":"Anti-cancer effects of polyphenols via targeting p53 signaling pathway: Updates and future directions","volume":"38","author":"Khan","year":"2020","journal-title":"Biotechnol. Adv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1134\/S0003683818030146","article-title":"Natural polyphenols: Biological activity, pharmacological potential, means of metabolic engineering (Review)","volume":"54","author":"Teplova","year":"2018","journal-title":"Appl. Biochem. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Garc\u00eda, C., S\u00e1nchez-Quesada, C., and Gaforio, J.J. (2019). Dietary flavonoids as cancer chemopreventive agents: An updated review of human studies. Antioxidants, 8.","DOI":"10.3390\/antiox8050137"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1002\/ptr.6403","article-title":"A review of molecular mechanisms involved in anticancer and antiangiogenic effects of natural polyphenolic compounds","volume":"33","author":"Abbaszadeh","year":"2019","journal-title":"Phytother. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.joim.2018.04.005","article-title":"Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum (L.)","volume":"16","author":"Patra","year":"2018","journal-title":"J. Integr. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2656","DOI":"10.1016\/j.fct.2010.06.036","article-title":"Antioxidant activities of leaf extract of Salvia miltiorrhiza Bunge and related phenolic constituents","volume":"48","author":"Zhang","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.abb.2019.02.011","article-title":"Antioxidant effect of resveratrol in single red blood cells measured by thermal fluctuation spectroscopy","volume":"665","author":"Gallardo","year":"2016","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.nutres.2006.06.004","article-title":"Acute intake of phenolic-rich juice improves antioxidant status in healthy subjects","volume":"26","author":"Ros","year":"2006","journal-title":"Nutr. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.cgh.2006.03.020","article-title":"Combination treatment with curcumin and quercetin of adenomas in familial Adenomatous polyposis","volume":"4","author":"Shoskes","year":"2006","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"208","DOI":"10.3109\/07357907.2010.550592","article-title":"Upregulation of p53 expression in patients with colorectal cancer by administration of curcumin","volume":"29","author":"He","year":"2011","journal-title":"Cancer Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11103","DOI":"10.1002\/jcp.27761","article-title":"Synergistic anticancer action of quercetin and curcumin against triple-negative breast cancer cell lines","volume":"234","author":"Kundur","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"961","DOI":"10.2741\/4761","article-title":"The antitumor effect of resveratrol on nasopharyngeal carcinoma cells","volume":"24","author":"Xiong","year":"2019","journal-title":"Front. Biosci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/tox.22677","article-title":"Naringenin inhibited migration and invasion of glioblastoma cells through multiple mechanisms","volume":"34","author":"Chen","year":"2019","journal-title":"Environ. Toxicol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/j.clnu.2014.12.019","article-title":"Antioxidant and anti-inflammatory effects of curcuminoid-piperine combination in subjects with metabolic syndrome: A randomized controlled trial and an updated meta-analysis","volume":"34","author":"Panahi","year":"2015","journal-title":"Clin. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.neuropharm.2016.02.019","article-title":"Naringenin reduces inflammatory pain in mice","volume":"105","author":"Zarpelon","year":"2016","journal-title":"Neuropharmacology"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Porro, C., Cianciulli, A., Trotta, T., Lofrumento, D.D., and Panaro, M.A. (2019). Curcumin regulates anti-inflammatory responses by JAK\/STAT\/SOCS signaling pathway in BV-2 microglial cells. Biology, 8.","DOI":"10.3390\/biology8030051"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Lecher, J.C., Diep, N., Krug, P.W., and Hilliard, J.K. (2019). Genistein has antiviral activity against herpes B virus and acts synergistically with antiviral treatments to reduce effective dose. Viruses, 11.","DOI":"10.3390\/v11060499"},{"key":"ref_27","first-page":"1","article-title":"Antiviral properties of resveratrol against pseudorabies virus are associated with the inhibition of I\u03baB kinase activation","volume":"7","author":"Zhao","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.micpath.2019.03.025","article-title":"A review of the current evidence of fruit phenolic compounds as potential antimicrobials against pathogenic bacteria","volume":"130","author":"Lima","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.ijantimicag.2018.04.024","article-title":"Plant nutraceuticals as antimicrobial agents in food preservation: Terpenoids, polyphenols and thiols","volume":"52","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12974-016-0779-0","article-title":"Resveratrol regulates neuro-inflammation and induces adaptive immunity in Alzheimer\u2019s disease","volume":"14","author":"Moussa","year":"2017","journal-title":"J. Neuroinflamm."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.jagp.2017.10.010","article-title":"Memory and brain amyloid and Tau effects of a bioavailable form of curcumin in non-demented adults: A double-blind, placebo-controlled 18-month trial","volume":"26","author":"Small","year":"2018","journal-title":"Am. J. Geriatr. Psychiatry"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6241017","DOI":"10.1155\/2018\/6241017","article-title":"Rutin as a potent antioxidant: Implications for neurodegenerative disorders","volume":"2018","author":"Enogieru","year":"2018","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.phymed.2010.01.007","article-title":"A randomized, double-blind, placebo-controlled, cross-over pilot study on the use of a standardized hop extract to alleviate menopausal discomforts","volume":"17","author":"Erkkola","year":"2010","journal-title":"Phytomedicine"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1002\/fsn3.109","article-title":"Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7","volume":"2","author":"Luo","year":"2014","journal-title":"Food Sci. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fbioe.2018.00032","article-title":"Metabolic engineering of the shikimate pathway for production of aromatics and derived compounds\u2014Present and future strain construction strategies","volume":"6","author":"Averesch","year":"2018","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/iub.1005","article-title":"Type III polyketide synthases in natural product biosynthesis","volume":"64","author":"Yu","year":"2012","journal-title":"Iubmb Life"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fpls.2019.00821","article-title":"Formation of flavonoid metabolons: Functional significance of protein-protein interactions and impact on flavonoid chemodiversity","volume":"10","author":"Nakayama","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/biof.1197","article-title":"Evaluation of the bioactive properties of avenanthramide analogs produced in recombinant yeast","volume":"41","author":"Moglia","year":"2015","journal-title":"BioFactors"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13036-019-0174-3","article-title":"Elucidation of the biosynthesis pathway and heterologous construction of a sustainable route for producing umbelliferone","volume":"13","author":"Zhao","year":"2019","journal-title":"J. Biol. Eng."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Satake, H., Shiraishi, A., Koyama, T., Matsumoto, E., Morimoto, K., Bahabadi, S.E., Ono, E., and Murata, J. (2017). Lignan biosynthesis for food bioengineering. Food Biosynth., 351\u2013379.","DOI":"10.1016\/B978-0-12-811372-1.00012-9"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.copbio.2015.11.003","article-title":"Microbial production of value-added nutraceuticals","volume":"37","author":"Wang","year":"2016","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1128\/MMBR.00031-14","article-title":"Heterologous production of curcuminoids","volume":"79","author":"Rodrigues","year":"2015","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1467-7652.2011.00664.x","article-title":"Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules","volume":"10","author":"Wilson","year":"2012","journal-title":"Plant Biotechnol. J."},{"key":"ref_44","first-page":"71","article-title":"Microbial Hosts as a Promising Platform for Polyphenol Production","volume":"Volume 1","author":"Akhtar","year":"2019","journal-title":"Natural Bio-active Compounds"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cpet.21","article-title":"Saccharomyces cerevisiae: A unicellular model genetic organism of enduring importance","volume":"16","author":"Hanson","year":"2018","journal-title":"Curr. Protoc. Essent. Lab. Tech."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/9780470559277.ch130191","article-title":"Manipulating natural product biosynthetic pathways via DNA assembler","volume":"6","author":"Shao","year":"2014","journal-title":"Curr. Protoc. Chem. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.ymben.2015.08.003","article-title":"Establishment of a yeast platform strain for production of p-coumaric acid through metabolic engineering of aromatic amino acid biosynthesis","volume":"31","author":"Rodriguez","year":"2015","journal-title":"Metab. Eng."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.ymben.2017.10.013","article-title":"Comparison of the metabolic response to over-production of p-coumaric acid in two yeast strains","volume":"44","author":"Rodriguez","year":"2017","journal-title":"Metab. Eng."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-019-1244-4","article-title":"Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose","volume":"18","author":"Borja","year":"2019","journal-title":"Microb. Cell Factories"},{"key":"ref_50","first-page":"1","article-title":"Rewiring carbon metabolism in yeast for high level production of aromatic chemicals","volume":"10","author":"Liu","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.eng.2018.11.029","article-title":"Engineering the biosynthesis of caffeic acid in Saccharomyces cerevisiae with heterologous enzyme combinations","volume":"5","author":"Liu","year":"2019","journal-title":"Engineering"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1021\/acssynbio.9b00431","article-title":"De novo biosynthesis of caffeic acid from glucose by engineered Saccharomyces cerevisiae","volume":"9","author":"Li","year":"2020","journal-title":"ACS Synth. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S1567-1356(03)00157-0","article-title":"Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol","volume":"4","author":"Becker","year":"2003","journal-title":"FEMS Yeast Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5670","DOI":"10.1128\/AEM.00609-06","article-title":"Production of resveratrol in recombinant microorganisms","volume":"72","author":"Beekwilder","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3361","DOI":"10.1128\/AEM.02796-09","article-title":"Considerable increase in resveratrol production by recombinant industrial yeast strains with use of rich medium","volume":"76","author":"Sydor","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.enzmictec.2010.09.004","article-title":"Production of resveratrol from p-coumaric acid in recombinant Saccharomyces cerevisiae expressing 4-coumarate:coenzyme A ligase and stilbene synthase genes","volume":"48","author":"Shin","year":"2011","journal-title":"Enzym. Microb. Technol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.ymben.2011.04.005","article-title":"Stepwise increase of resveratrol biosynthesis in yeast Saccharomyces cerevisiae by metabolic engineering","volume":"13","author":"Wang","year":"2011","journal-title":"Metab. Eng."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.enzmictec.2012.06.005","article-title":"Production of resveratrol from tyrosine in metabolically engineered Saccharomyces Cerevisiae","volume":"51","author":"Shin","year":"2012","journal-title":"Enzym. Microb. Technol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.jbiotec.2011.11.003","article-title":"Synthetic scaffolds increased resveratrol biosynthesis in engineered yeast cells","volume":"157","author":"Wang","year":"2012","journal-title":"J. Biotechnol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ymben.2015.08.007","article-title":"De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae","volume":"32","author":"Li","year":"2015","journal-title":"Metab. Eng."},{"key":"ref_61","first-page":"1","article-title":"Engineering yeast for high-level production of stilbenoid antioxidants","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1007\/s13213-014-0922-z","article-title":"Pterostilbene production by microorganisms expressing resveratrol O-methyltransferase","volume":"65","author":"Wang","year":"2015","journal-title":"Ann. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2962","DOI":"10.1128\/AEM.71.6.2962-2969.2005","article-title":"Metabolic engineering of the phenylpropanoid pathway in Saccharomyces cerevisiae","volume":"71","author":"Jiang","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2859-11-155","article-title":"De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae","volume":"11","author":"Koopman","year":"2012","journal-title":"Microb. Cell Factories"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.ymben.2009.07.004","article-title":"Metabolic engineering of the complete pathway leading to heterologous biosynthesis of various flavonoids and stilbenoids in Saccharomyces Cerevisiae","volume":"11","author":"Trantas","year":"2009","journal-title":"Metab. Eng."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1016\/j.biortech.2017.06.043","article-title":"Metabolic engineering of yeast for fermentative production of flavonoids","volume":"245","author":"Rodriguez","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"6638","DOI":"10.1021\/acs.jafc.7b02507","article-title":"Enhancement of naringenin biosynthesis from tyrosine by metabolic engineering of Saccharomyces cerevisiae","volume":"65","author":"Lyu","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-017-0774-x","article-title":"Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae","volume":"16","author":"Duan","year":"2017","journal-title":"Microb. Cell Factories"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"5596","DOI":"10.1021\/acs.jafc.9b01329","article-title":"Metabolic engineering of Saccharomyces cerevisiae for de Novo production of kaempferol","volume":"67","author":"Lyu","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"13478","DOI":"10.1021\/acs.jafc.9b01367","article-title":"Toward developing a yeast cell factory for the production of prenylated flavonoids","volume":"67","author":"Levisson","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.apsb.2019.07.005","article-title":"De novo biosynthesis of liquiritin in Saccharomyces cerevisiae","volume":"10","author":"Yin","year":"2019","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.ymben.2016.10.019","article-title":"Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties","volume":"39","author":"Eichenberger","year":"2017","journal-title":"Metab. Eng."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/femsyr\/foy046","article-title":"De novo biosynthesis of anthocyanins in Saccharomyces cerevisiae","volume":"18","author":"Eichenberger","year":"2018","journal-title":"FEMS Yeast Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"8241","DOI":"10.1128\/AEM.71.12.8241-8248.2005","article-title":"Investigation of two distinct flavone synthases for plant-specific flavone biosynthesis in Saccharomyces cerevisiae","volume":"71","author":"Leonard","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1021\/acs.jafc.9b07916","article-title":"Engineering Saccharomyces cerevisiae coculture platform for the production of flavonoids","volume":"68","author":"Du","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1002\/yea.3228","article-title":"Saccharomyces cerevisiae shuttle vectors","volume":"34","author":"Rudolf","year":"2017","journal-title":"Yeast"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/1567-1364.12016","article-title":"Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications","volume":"13","author":"Karim","year":"2013","journal-title":"FEMS Yeast Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1021\/bp970055d","article-title":"Sequential \u03b4-integration for the regulated insertion of cloned genes in Saccharomyces cerevisiae","volume":"13","author":"Lee","year":"1997","journal-title":"Biotechnol. Prog."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1021\/sb500079f","article-title":"Combinatorial assembly of large biochemical pathways into yeast chromosomes for improved production of value-added compounds","volume":"4","author":"Yuan","year":"2015","journal-title":"ACS Synth. Biol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"4336","DOI":"10.1093\/nar\/gkt135","article-title":"Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems","volume":"41","author":"DiCarlo","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ymben.2015.10.011","article-title":"A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae","volume":"33","author":"Shi","year":"2016","journal-title":"Metab. Eng."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1046\/j.1365-313X.1999.00491.x","article-title":"Three 4-coumarate:coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms","volume":"19","author":"Ehlting","year":"1999","journal-title":"Plant J."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-018-0951-6","article-title":"Engineering de novo anthocyanin production in Saccharomyces cerevisiae","volume":"17","author":"Levisson","year":"2018","journal-title":"Microb. Cell Factories"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms12851","article-title":"Dual regulation of cytoplasmic and mitochondrial acetyl-CoA utilization for improved isoprene production in Saccharomyces cerevisiae","volume":"7","author":"Lv","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.ymben.2011.05.001","article-title":"Harnessing yeast subcellular compartments for the production of plant terpenoids","volume":"13","author":"Farhi","year":"2011","journal-title":"Metab. Eng."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"13030","DOI":"10.1021\/ja0622094","article-title":"Using unnatural protein fusions to engineer resveratrol biosynthesis in yeast and mammalian cells","volume":"128","author":"Zhang","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"4058","DOI":"10.1021\/acs.biochem.9b00721","article-title":"Energy and enzyme activity landscapes of yeast chorismate mutase at cellular concentrations of allosteric effectors","volume":"58","author":"Gorman","year":"2019","journal-title":"Biochemistry"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1016\/j.cell.2016.02.004","article-title":"Engineering cellular metabolism","volume":"164","author":"Nielsen","year":"2016","journal-title":"Cell"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1021\/acssynbio.6b00346","article-title":"Increasing malonyl-CoA derived product through controlling the transcription regulators of phospholipid synthesis in Saccharomyces Cerevisiae","volume":"6","author":"Chen","year":"2017","journal-title":"ACS Synth. Biol."},{"key":"ref_90","first-page":"1","article-title":"Screening phosphorylation site mutations in yeast acetyl-CoA carboxylase using malonyl-CoA sensor to improve malonyl-CoA-derived product","volume":"9","author":"Chen","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mBio.01130-14","article-title":"Improving production of malonyl coenzyme A-derived metabolites by abolishing snf1-dependent regulation of Acc1","volume":"5","author":"Shi","year":"2014","journal-title":"mBio"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.2307\/3870059","article-title":"Stress-induced phenylpropanoid metabolism","volume":"7","author":"Dixon","year":"1995","journal-title":"Plant. Cell"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fbioe.2020.00059","article-title":"A combinatorial approach to optimize the production of curcuminoids from tyrosine in Escherichia coli","volume":"8","author":"Rodrigues","year":"2020","journal-title":"Front. Bioeng. Biotechnol."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/10\/5\/56\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:52:20Z","timestamp":1760363540000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/10\/5\/56"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,2]]},"references-count":93,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["life10050056"],"URL":"https:\/\/doi.org\/10.3390\/life10050056","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,2]]}}}