{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:07:09Z","timestamp":1767830829527,"version":"3.49.0"},"reference-count":49,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["iNOVA4Health\/MULTI\/04462\/2018"],"award-info":[{"award-number":["iNOVA4Health\/MULTI\/04462\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50025\/2020"],"award-info":[{"award-number":["UIDB\/50025\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/147518\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147518\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Poly(hydroxyalkanoates) (PHAs) with different material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate, P(3HB-co-3HV), with a 3HV of 25 wt.%, were used for the preparation of porous biopolymeric scaffolds. Solvent casting with particulate leaching (SCPL) and emulsion templating were evaluated to process these biopolymers in porous scaffolds. SCPL scaffolds were highly hydrophilic (&gt;170% swelling in water) but fragile, probably due to the increase of the polymer\u2019s polydispersity index and its high porosity (&gt;50%). In contrast, the emulsion templating technique resulted in scaffolds with a good compromise between porosity (27\u201349% porosity) and hydrophilicity (&gt;30% water swelling) and without impairing their mechanical properties (3.18\u20133.35 MPa tensile strength and 0.07\u20130.11 MPa Young\u2019s Modulus). These specifications are in the same range compared to other polymer-based scaffolds developed for tissue engineering. P(3HB-co-3HV) displayed the best overall properties, namely, lower crystallinity (11.3%) and higher flexibility (14.8% elongation at break. Our findings highlight the potency of our natural biopolyesters for the future development of novel porous scaffolds in tissue engineering, thanks also to their safety and biodegradability.<\/jats:p>","DOI":"10.3390\/life11090935","type":"journal-article","created":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T10:12:03Z","timestamp":1631095923000},"page":"935","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)"],"prefix":"10.3390","volume":"11","author":[{"given":"Asiyah","family":"Esmail","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, NOVA University Lisbon, 2780-157 Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"}]},{"given":"Jo\u00e3o R.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]},{"given":"Chantal","family":"Sevrin","sequence":"additional","affiliation":[{"name":"CEIB\u2014Interfaculty Research Centre of Biomaterials, University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}]},{"given":"Christian","family":"Grandfils","sequence":"additional","affiliation":[{"name":"CEIB\u2014Interfaculty Research Centre of Biomaterials, University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7733-8929","authenticated-orcid":false,"given":"Ugur Deneb","family":"Menda","sequence":"additional","affiliation":[{"name":"CENIMAT\/i3N, Department of Materials Science, Nova School of Sciences and Technology, Nova University Lisbon, 2819-516 Caparica, Portugal"}]},{"given":"Elvira","family":"Fortunato","sequence":"additional","affiliation":[{"name":"CENIMAT\/i3N, Department of Materials Science, Nova School of Sciences and Technology, Nova University Lisbon, 2819-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8320-0937","authenticated-orcid":false,"given":"Abel","family":"Oliva","sequence":"additional","affiliation":[{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, NOVA University Lisbon, 2780-157 Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-4640","authenticated-orcid":false,"given":"Filomena","family":"Freitas","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.ibiod.2017.10.001","article-title":"Polyhydroxyalkanoates: Characteristics, production, recent developments and applications","volume":"126","author":"Raza","year":"2018","journal-title":"Int. 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