{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:04:21Z","timestamp":1770750261571,"version":"3.50.0"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T00:00:00Z","timestamp":1642723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020; UIDB\/04378\/2020; LA\/P\/0140\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020; UIDB\/04378\/2020; LA\/P\/0140\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The yeast Pichia pastoris was cultivated under different dissolved oxygen (DO) levels (5, 15, 30 and 50% of the air saturation) to evaluate its impact on the production of the cell-wall polysaccharide chitin\u2013glucan complex (CGC) and mannans. Decreasing the DO level from 50 to 15% had no significant impact on cell growth but substrate conversion into biomass was improved. Under such conditions, a mannans content in the biomass of 22 wt% was reached, while the CGC content in the biomass was improved from 15 to 18 wt%, confirming that the DO level also impacted on P. pastoris cell-wall composition. Overall mannans and CGC volumetric productivity values of 10.69 and 8.67 g\/(L. day) were reached, respectively. On the other hand, the polymers\u2019 composition was not significantly affected by decreasing the DO level. These results demonstrated that considerable energy savings can be made in the polysaccharide production process by reducing the DO level during cultivation of P. pastoris by improving the overall polymers\u2019 productivity without altering their composition. This has impact on the polysaccharide production costs, which is of considerable relevance for process scale-up and products\u2019 commercialization.<\/jats:p>","DOI":"10.3390\/life12020161","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T08:37:18Z","timestamp":1642754238000},"page":"161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Influence of Dissolved Oxygen Level on Chitin\u2013Glucan Complex and Mannans Production by the Yeast Pichia pastoris"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8157-1731","authenticated-orcid":false,"given":"In\u00eas","family":"Farinha","sequence":"first","affiliation":[{"name":"Pharma 73, S.A., Edif\u00edcio Arcis, Rua Ivone Silva, 6, 4\u00ba Piso, 1050-124 Lisboa, Portugal"},{"name":"UCIBIO-Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]},{"given":"S\u00edlvia","family":"Baptista","sequence":"additional","affiliation":[{"name":"UCIBIO-Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"73100 Lda., Edif\u00edcio Arcis, Rua Ivone Silva, 6, 4\u00ba Piso, 1050-124 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4000-1836","authenticated-orcid":false,"given":"Maria A. M.","family":"Reis","sequence":"additional","affiliation":[{"name":"UCIBIO-Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-4640","authenticated-orcid":false,"given":"Filomena","family":"Freitas","sequence":"additional","affiliation":[{"name":"UCIBIO-Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1002\/jctb.6325","article-title":"Chitinous polymers: Extraction from fungal sources, characterization and processing towards value-added applications","volume":"95","author":"Ferreira","year":"2020","journal-title":"J. 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