{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T11:25:37Z","timestamp":1768821937849,"version":"3.49.0"},"reference-count":88,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T00:00:00Z","timestamp":1420675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT \u2013 Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BPD\/74894\/2010"],"award-info":[{"award-number":["SFRH\/BPD\/74894\/2010"]}]},{"name":"FCT \u2013 Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PTDC\/BIA-MIC\/2889\/2012-PR451402-BIL"],"award-info":[{"award-number":["PTDC\/BIA-MIC\/2889\/2012-PR451402-BIL"]}]},{"name":"S&amp;I","award":["EP\/E036252\/1"],"award-info":[{"award-number":["EP\/E036252\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>The interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is poorly studied, and the role of cyanobacterial exoproteins on cell wall biogenesis, morphology and even physiology is largely unknown. Here, we present a comprehensive examination of the Anabaena sp.  PCC 7120 exoproteome under various growth conditions. Altogether, 139 proteins belonging to 16 different functional categories have been identified. A large fraction (48%) of the identified proteins is classified as \u201chypothetical\u201d, falls into the \u201cother categories\u201d set or presents no similarity to other proteins. The evidence presented here shows that Anabaena sp. PCC 7120 is capable of outer membrane vesicle formation and that these vesicles are likely to contribute to the exoproteome profile. Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space. Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins.<\/jats:p>","DOI":"10.3390\/life5010130","type":"journal-article","created":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T11:29:32Z","timestamp":1420716572000},"page":"130-163","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-152X","authenticated-orcid":false,"given":"Paulo","family":"Oliveira","sequence":"first","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Porto 4150-180, Portugal"},{"name":"IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto 4150-180, Portugal"}]},{"given":"Nuno","family":"Martins","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Porto 4150-180, Portugal"},{"name":"IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto 4150-180, Portugal"},{"name":"Laboratoire du Biologie du Noyau, Institut de Biologie & de M\u00e9decine Mol\u00e9culaire,  Universit\u00e9 Libre de Bruxelles, Charleroi B-6041, Belgium"}]},{"given":"Marina","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Porto 4150-180, Portugal"},{"name":"IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto 4150-180, Portugal"}]},{"given":"Narciso","family":"Couto","sequence":"additional","affiliation":[{"name":"ChELSI Institute, Chemical and Biological Engineering, University of Sheffield, Sheffield S1 3JD, UK"}]},{"given":"Phillip","family":"Wright","sequence":"additional","affiliation":[{"name":"ChELSI Institute, Chemical and Biological Engineering, University of Sheffield, Sheffield S1 3JD, UK"}]},{"given":"Paula","family":"Tamagnini","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Porto 4150-180, Portugal"},{"name":"IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto 4150-180, Portugal"},{"name":"Faculdade de Ci\u00eancias, Departamento de Biologia, Universidade do Porto, Porto 4169-007, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2015,1,8]]},"reference":[{"key":"ref_1","unstructured":"Varki, A. 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