{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T03:13:49Z","timestamp":1771038829763,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T00:00:00Z","timestamp":1554940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"GAI 2013 (Faculdade de Medicina da Universidade de Coimbra); FCT, Portugal (Strategic Project PEst), COMPETE-FEDER.","award":["C\/SAU\/UI3282\/2013 e UID\/NEU\/04539\/2013"],"award-info":[{"award-number":["C\/SAU\/UI3282\/2013 e UID\/NEU\/04539\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life\u00ae), mineral trioxide aggregate (WhiteProRoot\u00aeMTA) and calcium silicate (Biodentine\u2122), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B (SRB) assay, and the type of death and cell cycle analysis by flow cytometry. Alkaline phosphatase was evaluated by polymerase chain reaction (PCR), and dentin sialoprotein expression was assessed by immunocytochemistry. Mineralization was determined by the Alizarin Red S colorimetric assay and quantified by spectrophotometry. (3) Results: Life\u00ae induced a decrease in metabolic activity and viability, which is associated with an increase cell death. WhiteProRoot\u00aeMTA and Biodentine\u2122 induced similar effects in cytotoxicity assays, with an increase in the expression of dentin sialoprotein (DSP) and formation of mineralized deposits, especially with Biodentine\u2122. (4) Conclusions: The results of WhiteProRoot\u00aeMTA confirm its indication for these therapies, justifying its recognition as the \u201cgold standard\u201d. Biodentine\u2122 may be an alternative, since they promote the same cellular response that mineral trioxide aggregate (MTA) does.<\/jats:p>","DOI":"10.3390\/ma12071184","type":"journal-article","created":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T03:46:37Z","timestamp":1555040797000},"page":"1184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Biodentine\u2122 Boosts, WhiteProRoot\u00aeMTA Increases and Life\u00ae Suppresses Odontoblast Activity"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6154-4173","authenticated-orcid":false,"given":"Anabela","family":"Paula","sequence":"first","affiliation":[{"name":"Institute of Integrated Clinical Practice, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0689-6007","authenticated-orcid":false,"given":"Mafalda","family":"Laranjo","sequence":"additional","affiliation":[{"name":"Biophysics Institute, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9269-5417","authenticated-orcid":false,"given":"Carlos Miguel","family":"Marto","sequence":"additional","affiliation":[{"name":"Institute Experimental Pathology, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"given":"Ana Margarida","family":"Abrantes","sequence":"additional","affiliation":[{"name":"Biophysics Institute, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"given":"Jo\u00e3o","family":"Casalta-Lopes","sequence":"additional","affiliation":[{"name":"Biophysics Institute, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Radiation Oncology Department, Coimbra University Hospital Center, 3000-548 Coimbra, Portugal"}]},{"given":"Ana Cristina","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laboratory of Oncobiology and Hematology, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"given":"Ana Bela","family":"Sarmento-Ribeiro","sequence":"additional","affiliation":[{"name":"Laboratory of Oncobiology and Hematology, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5968-6161","authenticated-orcid":false,"given":"Manuel M.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Institute of Endodontics, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7202-1650","authenticated-orcid":false,"given":"Maria Filomena","family":"Botelho","sequence":"additional","affiliation":[{"name":"Biophysics Institute, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI Consortium, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5759-5557","authenticated-orcid":false,"given":"Eunice","family":"Carrilho","sequence":"additional","affiliation":[{"name":"Institute of Integrated Clinical Practice, Institute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), CNC.IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0300-5712(02)00089-1","article-title":"Odontoblast morphology and dental repair","volume":"31","author":"Murray","year":"2003","journal-title":"J. Dent."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1365-2591.2012.02101.x","article-title":"Tertiary dentinogenesis with calcium hydroxide: A review of proposed mechanisms","volume":"46","author":"Sangwan","year":"2013","journal-title":"Int. Endod. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s10266-009-0116-5","article-title":"Can interaction of materials with the dentin-pulp complex contribute to dentin regeneration?","volume":"98","author":"Ferracane","year":"2010","journal-title":"Odontology"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Goldberg, M. (2014). The Dental Pulp, Springer.","DOI":"10.1007\/978-3-642-55160-4"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.cden.2016.08.008","article-title":"Clinical and Molecular Perspectives of Reparative Dentin Formation","volume":"61","author":"Song","year":"2017","journal-title":"Dent. Clin. North Am."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1177\/10454411930040050301","article-title":"Dentinogenesis","volume":"4","author":"Linde","year":"1993","journal-title":"Crit. Rev. Oral Biol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.bone.2009.06.018","article-title":"Molecular characterization of young and mature odontoblasts","volume":"45","author":"Simon","year":"2009","journal-title":"Bone"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1111\/j.1365-2591.1994.tb00233.x","article-title":"Mechanisms controlling secondary initiation of dentinogenesis: A review","volume":"27","author":"Tziafas","year":"1994","journal-title":"Int. Endod. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0300-5712(99)00047-0","article-title":"Designing new treatment strategies in vital pulp therapy","volume":"28","author":"Tziafas","year":"2000","journal-title":"J. Dent."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/j.1875-595X.2005.tb00326.x","article-title":"Calcium hydroxide: A review","volume":"55","author":"Farhad","year":"2005","journal-title":"Int. Dent. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/181945","article-title":"Cytocompatibility and Antibacterial Properties of Capping Materials","volume":"2014","author":"Poggio","year":"2014","journal-title":"Sci. World J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1007\/s00784-015-1466-8","article-title":"Dental discoloration caused by bismuth oxide in MTA in the presence of sodium hypochlorite","volume":"19","author":"Marciano","year":"2015","journal-title":"Clin. Oral Investig."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e170","DOI":"10.1016\/j.rodmex.2016.02.023","article-title":"Physicochemical analysis of MTA Angelus\u00ae and Biodentine\u00ae conducted with X ray difraction, dispersive energy spectrometry, X ray fluorescence, scanning electron microscope and infra red spectroscopy","volume":"19","author":"Rocha","year":"2015","journal-title":"Rev. Odontol. Mex."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"20","DOI":"10.2341\/09-038CR1","article-title":"Pulp capping with mineral trioxide aggregate (MTA): A retrospective analysis of carious pulp exposures treated by undergraduate dental students","volume":"35","author":"Miles","year":"2010","journal-title":"Oper. Dent."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1046\/j.1365-2591.2003.00652.x","article-title":"Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: A preliminary report","volume":"36","author":"Aeinehchi","year":"2003","journal-title":"Int. Endod. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"406","DOI":"10.4103\/1735-3327.166187","article-title":"Outcomes of vital pulp therapy in permanent teeth with different medicaments based on review of the literature","volume":"12","author":"Akhlaghi","year":"2015","journal-title":"Dent. Res. J. (Isfahan)"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.dental.2011.02.001","article-title":"\u201cMTA\u201d\u2014An Hydraulic Silicate Cement: Review update and setting reaction","volume":"27","author":"Darvell","year":"2011","journal-title":"Dent. Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.joen.2014.09.005","article-title":"A Randomized Controlled Study of the Use of ProRoot Mineral Trioxide Aggregate and Endocem as Direct Pulp Capping Materials","volume":"41","author":"Song","year":"2015","journal-title":"J. Endod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s40368-014-0114-3","article-title":"BiodentineTM material characteristics and clinical applications: A review of the literature","volume":"15","author":"Rajasekharan","year":"2014","journal-title":"Eur. Arch. Paediatr. Dent."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.joen.2013.11.006","article-title":"Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line","volume":"40","author":"Bosomworth","year":"2014","journal-title":"J. Endod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1155\/2014\/160951","article-title":"A review on biodentine, a contemporary dentine replacement and repair material","volume":"2014","author":"Malkondu","year":"2014","journal-title":"Biomed Res. Int."},{"key":"ref_22","unstructured":"(2019, April 10). ISO 10993-5:2009 Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity. Available online: https:\/\/www.iso.org\/standard\/36406.html."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.joen.2012.11.026","article-title":"In Vitro Cytotoxicity Evaluation of a Novel Root Repair Material","volume":"39","author":"Zhou","year":"2013","journal-title":"J. Endod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.joen.2012.03.020","article-title":"Effects of an Experimental Calcium Aluminosilicate Cement on the Viability of Murine Odontoblast-like Cells","volume":"38","author":"Wei","year":"2012","journal-title":"J. Endod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1038\/nprot.2006.179","article-title":"Sulforhodamine B colorimetric assay for cytotoxicity screening","volume":"1","author":"Vichai","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.pdpdt.2012.05.003","article-title":"2-Bromo-5-hydroxyphenylporphyrins for photodynamic therapy: Photosensitization efficiency, subcellular localization and in vivo studies","volume":"10","author":"Laranjo","year":"2013","journal-title":"Photodiagnosis Photodyn. Ther."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.joen.2015.12.018","article-title":"Expression of Mineralization Markers during Pulp Response to Biodentine and Mineral Trioxide Aggregate","volume":"42","author":"Faccioli","year":"2016","journal-title":"J. Endod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/j.dental.2012.09.011","article-title":"Odontogenic responses of human dental pulp cells to collagen\/nanobioactive glass nanocomposites","volume":"28","author":"Bae","year":"2012","journal-title":"Dent. Mater."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/S1348-8643(05)80014-X","article-title":"Differentiation of Rat Dental Pulp-derived Cells into an Osteoblastic Lineage","volume":"2","author":"Nozaki","year":"2005","journal-title":"Oral Sci. Int."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1159\/000075032","article-title":"Different Effects of BMP-2 on Marrow Stromal Cells from Human and Rat Bone","volume":"176","author":"Osyczka","year":"2004","journal-title":"Cells Tissues Organs"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1016\/j.bone.2012.01.005","article-title":"The enhancement of osteogenesis through the use of dental pulp pluripotent stem cells in 3D","volume":"50","author":"Atari","year":"2012","journal-title":"Bone"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Cai, S., Zhang, W., and Chen, W. (2016). PDGFR\u03b2+\/c-kit+ pulp cells are odontoblastic progenitors capable of producing dentin-like structure in vitro and in vivo. BMC Oral Health, 16.","DOI":"10.1186\/s12903-016-0307-8"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.dental.2015.11.004","article-title":"Dentin matrix components extracted with phosphoric acid enhance cell proliferation and mineralization","volume":"32","author":"Salehi","year":"2016","journal-title":"Dent. Mater."},{"key":"ref_34","first-page":"1","article-title":"Biocompatibility of Novel Type I Collagen Purified from Tilapia Fish Scale: An In Vitro Comparative Study","volume":"2015","author":"Tang","year":"2015","journal-title":"Biomed Res. Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1016\/j.joen.2010.08.052","article-title":"Tertiary Dentin Formation after Direct Pulp Capping with Odontogenic Ameloblast-associated Protein in Rat Teeth","volume":"36","author":"Yang","year":"2010","journal-title":"J. Endod."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.joen.2010.11.009","article-title":"Zinc Chloride for Odontogenesis of Dental Pulp Stem Cells via Metallothionein Up-regulation","volume":"37","author":"Lin","year":"2011","journal-title":"J. Endod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.joen.2012.01.015","article-title":"Low-intensity Low-frequency Ultrasound Promotes Proliferation and Differentiation of Odontoblast-like Cells","volume":"38","author":"Man","year":"2012","journal-title":"J. Endod."},{"key":"ref_38","unstructured":"Wang, Y. (2010). Vascular Biology of the Placenta, Morgan & Claypool Life Sciences."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1159\/000151455","article-title":"Effect of dentin phosphoprotein on phosphate-induced apoptosis of odontoblast-like cells","volume":"189","author":"Fujisawa","year":"2009","journal-title":"Cells Tissues Organs"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.joen.2013.07.009","article-title":"In Vitro Biocompatibility and Oxidative Stress Profiles of Different Hydraulic Calcium Silicate Cements","volume":"40","author":"Eid","year":"2014","journal-title":"J. Endod."},{"key":"ref_41","first-page":"2515","article-title":"Tetrazolium-based assays for cellular viability: A critical examination of selected parameters affecting formazan production","volume":"51","author":"Vistica","year":"1991","journal-title":"Cancer Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1002\/ijc.24998","article-title":"Database of misidentified cell lines","volume":"126","author":"Freshney","year":"2010","journal-title":"Int. J. cancer"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1016\/j.joen.2010.02.013","article-title":"Effects of mineral trioxide aggregate on human dental pulp cells after pulp-capping procedures","volume":"36","author":"Paranjpe","year":"2010","journal-title":"J. Endod."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1016\/j.joen.2008.06.007","article-title":"The Effect of Mineral Trioxide Aggregate on the Mineralization Ability of Rat Dental Pulp Cells: An In Vitro Study","volume":"34","author":"Yasuda","year":"2008","journal-title":"J. Endod."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"86","DOI":"10.5603\/FHC.a2017.0008","article-title":"Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials\u2014an in vitro study","volume":"55","author":"Pawinska","year":"2017","journal-title":"Folia Histochem. Cytobiol."},{"key":"ref_46","first-page":"69","article-title":"Biocompatibility of a new pulp capping cement","volume":"5","author":"Poggio","year":"2014","journal-title":"Ann. Stomatol. (Roma)"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1365-2591.2008.01506.x","article-title":"Cytotoxicity and genotoxicity of pulp capping materials in two cell lines","volume":"42","author":"Camargo","year":"2009","journal-title":"Int. Endod. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/j.biocel.2007.04.007","article-title":"Superoxide anion: Oncogenic reactive oxygen species?","volume":"39","author":"Pervaiz","year":"2007","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.joen.2012.04.018","article-title":"Biodentine induces immortalized murine pulp cell differentiation into odontoblast-like cells and stimulates biomineralization","volume":"38","author":"Zanini","year":"2012","journal-title":"J. Endod."},{"key":"ref_50","first-page":"298","article-title":"Direct pulp capping: What is the most effective therapy?\u2014Systematic review and meta-analysis","volume":"4","author":"Laranjo","year":"2018","journal-title":"J. Evid. Based Dent. Pract."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"129","DOI":"10.4103\/0972-0707.212247","article-title":"A comparison of human dental pulp response to calcium hydroxide and Biodentine as direct pulp-capping agents","volume":"20","author":"Jalan","year":"2017","journal-title":"J. Conserv. Dent."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1016\/j.joen.2015.07.015","article-title":"Gene Expression Profiling and Molecular Signaling of Dental Pulp Cells in Response to Tricalcium Silicate Cements: A Systematic Review","volume":"41","author":"Rathinam","year":"2015","journal-title":"J. Endod."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1016\/j.joen.2016.08.027","article-title":"Gene Expression Profiling and Molecular Signaling of Various Cells in Response to Tricalcium Silicate Cements: A Systematic Review","volume":"42","author":"Rathinam","year":"2016","journal-title":"J. Endod."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Loison-Robert, L.S., Tassin, M., Bonte, E., Berbar, T., Isaac, J., Berdal, A., Simon, S., and Fournier, B.P.J. (2018). In vitro effects of two silicate-based materials, Biodentine and BioRoot RCS, on dental pulp stem cells in models of reactionary and reparative dentinogenesis. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0190014"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s00784-015-1515-3","article-title":"Three-dimensional culture of dental pulp stem cells in direct contact to tricalcium silicate cements","volume":"20","author":"Widbiller","year":"2016","journal-title":"Clin. Oral Investig."}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/12\/7\/1184\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:44:33Z","timestamp":1760186673000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/12\/7\/1184"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,11]]},"references-count":55,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2019,4]]}},"alternative-id":["ma12071184"],"URL":"https:\/\/doi.org\/10.3390\/ma12071184","relation":{},"ISSN":["1996-1944"],"issn-type":[{"value":"1996-1944","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,11]]}}}