{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T18:36:31Z","timestamp":1766428591495,"version":"build-2065373602"},"reference-count":18,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012268","name":"Proteomass Scientific Society","doi-asserted-by":"publisher","award":["General funds 2019-2020"],"award-info":[{"award-number":["General funds 2019-2020"]}],"id":[{"id":"10.13039\/501100012268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Associate Laboratory Research Unit for Green Chemistry - Clean Processes and Technologies \u2013 LAQV-REQUIMTE","award":["POCI-01-0145-FEDER \u2013 007265"],"award-info":[{"award-number":["POCI-01-0145-FEDER \u2013 007265"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/QEQ-MED\/2118\/2014","Research Grant JFL PTDC\/QEQ-MED\/2118\/2014"],"award-info":[{"award-number":["PTDC\/QEQ-MED\/2118\/2014","Research Grant JFL PTDC\/QEQ-MED\/2118\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Non-viral gene delivery using exogenous microRNAs is a potential strategy for fighting cancers with poor prognosis and which lack specific therapies, such as triple-negative breast cancer (TNBC). Herein we report the synthesis of six nontoxic electrostatic polymeric nanocapsules (P1 to P6) for microRNA delivery in TNBC cells. 1H Nuclear Magnetic Resonance (NMR) spectroscopy and Scanning Electron Microscopy (SEM) were used to characterize the nanopolyplexes, synthesized with Poly(L-Lysine) and hyaluronic acid (Ha). Studies on the activity of the ternary HA\/PLI\/miRNA-34 nanopolyplexes towards TNBC cell line MDA-MB-231 were conducted. The nanopolyplexes mediated intracellular restoration of tumor suppressor miR34a was evaluated by using Western blotting to quantify the expression level of the Bcl-2 protein. The results suggest that the P5, with a ratio PLI\/Ha of 0.05, was the most promising for the delivery of miR-34a into TNBC cells; the P5 nanocapsules were able to reduce Bcl-2 expression at a protein level, and had an effect in the overall cell viability after 24 h treatment.<\/jats:p>","DOI":"10.3390\/ma13235309","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T09:06:28Z","timestamp":1606208788000},"page":"5309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Study and Preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the Effective Delivery of Tumor Suppressive MiR-34a into Triple-Negative Breast Cancer Cells"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2817-0338","authenticated-orcid":false,"given":"Jamila","family":"Djafari","sequence":"first","affiliation":[{"name":"BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, Caparica Campus, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8853-2631","authenticated-orcid":false,"given":"Javier","family":"Fern\u00e1ndez-Lodeiro","sequence":"additional","affiliation":[{"name":"BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, Caparica Campus, 2829-516 Caparica, Portugal"},{"name":"PROTEOMASS Scientific Society, Rua dos Inventores, Madam Parque, Caparica Campus, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6032-8679","authenticated-orcid":false,"given":"Hugo M.","family":"Santos","sequence":"additional","affiliation":[{"name":"BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, Caparica Campus, 2829-516 Caparica, Portugal"},{"name":"PROTEOMASS Scientific Society, Rua dos Inventores, Madam Parque, Caparica Campus, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5659-6008","authenticated-orcid":false,"given":"Julia","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Institut de Biotecnologia i de Biomedicina, Universitat Aut\u00f2noma de Barcelona, Campus Universitari, 08193 Bellaterra, Barcelona, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8849-1101","authenticated-orcid":false,"given":"Sergi","family":"Rodriguez-Calado","sequence":"additional","affiliation":[{"name":"Institut de Biotecnologia i de Biomedicina, Universitat Aut\u00f2noma de Barcelona, Campus Universitari, 08193 Bellaterra, Barcelona, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1633-8212","authenticated-orcid":false,"given":"Emilia","family":"B\u00e9rtolo","sequence":"additional","affiliation":[{"name":"Section of Natural and Applied Sciences, Canterbury Christ Church University, Canterbury CT1 1QU, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6276-8507","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"Capelo-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, Caparica Campus, 2829-516 Caparica, Portugal"},{"name":"PROTEOMASS Scientific Society, Rua dos Inventores, Madam Parque, Caparica Campus, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5582-5446","authenticated-orcid":false,"given":"Carlos","family":"Lodeiro","sequence":"additional","affiliation":[{"name":"BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, Caparica Campus, 2829-516 Caparica, Portugal"},{"name":"PROTEOMASS Scientific Society, Rua dos Inventores, Madam Parque, Caparica Campus, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1200\/JCO.2005.05.2308","article-title":"Patterns of cancer incidence, mortality, and prevalence across five continents: Defining priorities to reduce cancer disparities in different geographic regions of the world","volume":"24","author":"Kamangar","year":"2006","journal-title":"J. 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