{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:43:54Z","timestamp":1771663434700,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T00:00:00Z","timestamp":1606262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["10168064"],"award-info":[{"award-number":["10168064"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"German Research Foundation","award":["GE2512\/2-2"],"award-info":[{"award-number":["GE2512\/2-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Loosening of orthodontic and orthopedic implants is a critical and common clinical problem. To minimize the numbers of revision surgeries due to peri-implant inflammation or insufficient osseointegration, developments of new implant manufacturing strategies are indicated. Ultrafast laser surface texturing is a promising contact-free technology to modify the physicochemical properties of surfaces toward an anti-infectious functionalization. This work aims to texture Ti6Al4V surfaces with ultraviolet (UV) and green (GR) radiation for the manufacturing of laser-induced periodic surface structures (LIPSS). The assessment of these surface modifications addresses key aspects of topography, morphology and chemical composition. Human primary mesenchymal stromal cells (hMSCs) were cultured on laser-textured and polished Ti6Al4V to characterize the surfaces in terms of their in vitro biocompatibility, cytotoxicity, and metal release. The outcomes of the in vitro experiment show the successful culture of hMSCs on textured Ti6Al4V surfaces developed within this work. Cells cultured on LIPSS surfaces were not compromised in terms of their viability if compared to polished surfaces. Yet, the hMSC culture on UV-LIPSS show significantly lower lactate dehydrogenase and titanium release into the supernatant compared to polished. Thus, the presented surface modification can be a promising approach for future applications in orthodontics and orthopedics.<\/jats:p>","DOI":"10.3390\/ma13235342","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T20:48:12Z","timestamp":1606337292000},"page":"5342","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Preclinical In Vitro Assessment of Submicron-Scale Laser Surface Texturing on Ti6Al4V"],"prefix":"10.3390","volume":"13","author":[{"given":"Luiz","family":"Schweitzer","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Production Systems and Design Technology, Pascalstr. 8-9, 10587 Berlin, Germany"},{"name":"Department of Orthopedics and Orthopedic Surgery, University Medicine Greifswald, 17475 Greifswald, Germany"}]},{"given":"Alexandre","family":"Cunha","sequence":"additional","affiliation":[{"name":"SENAI Innovation Institute in Manufacturing Systems and Laser Processing, Rua Arno Waldemar D\u00f6hler 308, Joinville, 89218-153 Santa Catarina, Brazil"},{"name":"Genetoo Inc., 9841 Washingtonian Blvd, Suite 200, Gaithersburg, MD 20878, USA"}]},{"given":"Thiago","family":"Pereira","sequence":"additional","affiliation":[{"name":"SENAI Innovation Institute in Manufacturing Systems and Laser Processing, Rua Arno Waldemar D\u00f6hler 308, Joinville, 89218-153 Santa Catarina, Brazil"}]},{"given":"Kerstin","family":"Mika","sequence":"additional","affiliation":[{"name":"Julius Wolff Institute, Charit\u00e9 Universit\u00e4tsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"},{"name":"Berlin Institute of Health Center for Regenerative Therapies, Charit\u00e9 Universit\u00e4tsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3131-4219","authenticated-orcid":false,"given":"Ana Maria","family":"Botelho do Rego","sequence":"additional","affiliation":[{"name":"BSIRG, Departamento de Engenharia Qu\u00edmica, iBB-Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6784-6540","authenticated-orcid":false,"given":"Ana Maria","family":"Ferraria","sequence":"additional","affiliation":[{"name":"BSIRG, Departamento de Engenharia Qu\u00edmica, iBB-Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal"}]},{"given":"Heinz","family":"Kieburg","sequence":"additional","affiliation":[{"name":"Laser-Mikrotechnologie Dr. Kieburg, James-Frank-Str. 15, 12489 Berlin, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8750-6324","authenticated-orcid":false,"given":"Sven","family":"Geissler","sequence":"additional","affiliation":[{"name":"Julius Wolff Institute, Charit\u00e9 Universit\u00e4tsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"},{"name":"Berlin Institute of Health Center for Regenerative Therapies, Charit\u00e9 Universit\u00e4tsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"}]},{"given":"Eckart","family":"Uhlmann","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Production Systems and Design Technology, Pascalstr. 8-9, 10587 Berlin, Germany"},{"name":"Institute for Machine Tools and Factory Management, Technische Universit\u00e4t Berlin, Pascalstr. 8-9, 10587 Berlin, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2337-0795","authenticated-orcid":false,"given":"Janosch","family":"Schoon","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Orthopedic Surgery, University Medicine Greifswald, 17475 Greifswald, Germany"},{"name":"Julius Wolff Institute, Charit\u00e9 Universit\u00e4tsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/S0140-6736(14)61798-0","article-title":"Periprosthetic joint infection","volume":"387","author":"Kapadia","year":"2016","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1902\/jop.2009.090269","article-title":"Prevalence of peri-implantitis related to severity of the disease with different degrees of bone loss","volume":"81","author":"Koldsland","year":"2010","journal-title":"J. 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