{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T04:14:36Z","timestamp":1771647276456,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["PROMETEO\/2016\/063"],"award-info":[{"award-number":["PROMETEO\/2016\/063"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>The development of three-dimensional environments to mimic the in vivo cellular response is a problem in the building of disease models. This study aimed to synthesize and validate three-dimensional support for culturing monoclonal plasma cells (mPCs) as a disease model for multiple myeloma. The three-dimensional environment is a biomimetic microgel formed by alginate microspheres and produced on a microfluidic device whose surface has been functionalized by a layer-by-layer process with components of the bone marrow\u2019s extracellular matrix, which will interact with mPC. As a proof of concept, RPMI 8226 cell line cells were cultured in our 3D culture platform. We proved that hyaluronic acid significantly increased cell proliferation and corroborated its role in inducing resistance to dexamethasone. Despite collagen type I having no effect on proliferation, it generated significant resistance to dexamethasone. Additionally, it was evidenced that both biomolecules were unable to induce resistance to bortezomib. These results validate the functionalized microgels as a 3D culture system that emulates the interaction between tumoral cells and the bone marrow extracellular matrix. This 3D environment could be a valuable culture system to test antitumoral drugs efficiency in multiple myeloma.<\/jats:p>","DOI":"10.3390\/ma14237121","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T05:02:36Z","timestamp":1638334956000},"page":"7121","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Biomimetic 3D Environment Based on Microgels as a Model for the Generation of Drug Resistance in Multiple Myeloma"],"prefix":"10.3390","volume":"14","author":[{"given":"Juan Carlos","family":"Mar\u00edn-Pay\u00e1","sequence":"first","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}]},{"given":"Blanca","family":"D\u00edaz-Benito","sequence":"additional","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}]},{"given":"Luis Amaro","family":"Martins","sequence":"additional","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6455-2993","authenticated-orcid":false,"given":"Sandra Clara","family":"Trujillo","sequence":"additional","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"},{"name":"Biomedical Research Networking, Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Valencia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8808-3423","authenticated-orcid":false,"given":"Lourdes","family":"Cord\u00f3n","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Hematolog\u00eda, Instituto de Investigaci\u00f3n Sanitaria La Fe (IISLAFE), 46026 Valencia, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer (CIBERONC), Instituto Carlos III, 28029 Madrid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6791-7620","authenticated-orcid":false,"given":"Senentxu","family":"Lanceros-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV\/EHU Science Park, 48940 Leioa, Spain"},{"name":"IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain"},{"name":"Physics Centre, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2428-0903","authenticated-orcid":false,"given":"Gloria","family":"Gallego Ferrer","sequence":"additional","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"},{"name":"Biomedical Research Networking, Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Valencia, Spain"}]},{"given":"Amparo","family":"Sempere","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Hematolog\u00eda, Instituto de Investigaci\u00f3n Sanitaria La Fe (IISLAFE), 46026 Valencia, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer (CIBERONC), Instituto Carlos III, 28029 Madrid, Spain"},{"name":"Haematology Department, Hospital Universitari i Polit\u00e8cnic La Fe, 46026 Valencia, Spain"}]},{"given":"Jos\u00e9 Luis","family":"G\u00f3mez Ribelles","sequence":"additional","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"},{"name":"Biomedical Research Networking, Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Valencia, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1080\/10428190500051448","article-title":"Extracellular matrix in bone marrow can mediate drug resistance in myeloma","volume":"46","author":"Vincent","year":"2005","journal-title":"Leuk. 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