{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:21:13Z","timestamp":1761218473580,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-030498"],"award-info":[{"award-number":["POCI-01-0145-FEDER-030498"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Two type of cells representing periodontal hard tissues (osteoblasts) and soft tissues (fibroblasts) were evaluated in response to microgroove-milled zirconia surfaces. A total of 90 zirconia discs were randomly assigned to four width-standardized milling microgroove-textured groups and a control group without grooves (UT). The sandblast and acid-etch protocol were applied to all samples. Both cell lines were cultured on zirconia discs from 1 day up to 14 days. Cell morphology and adhesion were evaluated after 1 day of culturing. Cell viability and proliferation of the cells were measured. Alkaline phosphatase activity, collagen I, osteopontin, interleukin 1\u03b2 and interleukin 8 secretions were assessed at predefined times. The results obtained were presented in the form of bar graphs as means and standard deviations. Multi comparisons between groups were evaluated using two-away ANOVA or Mann\u2013Whitney tests, and a p-value &lt; 0.05 was established. Group comparisons with regard to cell viability, proliferation and secretion of collagen I, interleukin-1\u03b2 and interleukin 8 revealed no statistically significant differences. The alkaline phosphatase activity and osteopontin secretion were significantly higher in the group with a large groove compared to the small one and the control group. Nevertheless, the viability of gingival and bone cells did not appear to be affected by the milled microgroove texture compared to the conventional sandblasted and acid-etched texture, but they seem to influence osteoblasts\u2019 cellular differentiation.<\/jats:p>","DOI":"10.3390\/ma15072481","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T21:41:30Z","timestamp":1648590090000},"page":"2481","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Human Gingival Fibroblast and Osteoblast Behavior on Groove-Milled Zirconia Implant Surfaces"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6798-0208","authenticated-orcid":false,"given":"Mariana Brito","family":"da Cruz","sequence":"first","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Medicina Dent\u00e1ria, Unidade de Investiga\u00e7\u00e3o em Ci\u00eancias Orais e Biom\u00e9dicas (UICOB), LIBPhys-FTC UID\/FIS\/04559\/2013, Rua Professora Teresa Ambr\u00f3sio, 1600-277 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8740-642X","authenticated-orcid":false,"given":"Joana Faria","family":"Marques","sequence":"additional","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Medicina Dent\u00e1ria, Unidade de Investiga\u00e7\u00e3o em Ci\u00eancias Orais e Biom\u00e9dicas (UICOB), LIBPhys-FTC UID\/FIS\/04559\/2013, Rua Professora Teresa Ambr\u00f3sio, 1600-277 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3454-065X","authenticated-orcid":false,"given":"Neusa","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Medicina Dent\u00e1ria, Unidade de Investiga\u00e7\u00e3o em Ci\u00eancias Orais e Biom\u00e9dicas (UICOB), Rua Professora Teresa Ambr\u00f3sio, 1600-277 Lisboa, Portugal"}]},{"given":"Sara","family":"Madeira","sequence":"additional","affiliation":[{"name":"Center for Microelectromechanical Systems (CMEMS), Department of Mechanical Engineering, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"given":"\u00d3scar","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Center for Microelectromechanical Systems (CMEMS), Department of Mechanical Engineering, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"given":"Filipe Samuel","family":"Silva","sequence":"additional","affiliation":[{"name":"Center for Microelectromechanical Systems (CMEMS), Department of Mechanical Engineering, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5544-3744","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Caram\u00eas","sequence":"additional","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Medicina Dent\u00e1ria, Unidade de Investiga\u00e7\u00e3o em Ci\u00eancias Orais e Biom\u00e9dicas (UICOB), LIBPhys-FTC UID\/FIS\/04559\/2013, Rua Professora Teresa Ambr\u00f3sio, 1600-277 Lisboa, Portugal"},{"name":"Universidade de Lisboa, Faculdade de Medicina Dent\u00e1ria, Bone Physiology Research Group, Rua Professora Teresa Ambr\u00f3sio, 1600-277 Lisboa, Portugal"}]},{"given":"Ant\u00f3nio","family":"Mata","sequence":"additional","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Medicina Dent\u00e1ria, Unidade de Investiga\u00e7\u00e3o em Ci\u00eancias Orais e Biom\u00e9dicas (UICOB), LIBPhys-FTC UID\/FIS\/04559\/2013, Rua Professora Teresa Ambr\u00f3sio, 1600-277 Lisboa, Portugal"},{"name":"Universidade de Lisboa, Faculdade de Medicina Dent\u00e1ria, Cochrane Portugal, Instituto de Sa\u00fade Baseada na Evid\u00eancia (ISBE), Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bressan, E., Ferroni, L., Gardin, C., Bellin, G., Sbricoli, L., Sivolella, S., Brunello, G., Schwartz-Arad, D., Mijiritsky, E., and Penarrocha, M. 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