{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T02:33:28Z","timestamp":1762050808923,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T00:00:00Z","timestamp":1652400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PD\/BD\/135258\/2017"],"award-info":[{"award-number":["PD\/BD\/135258\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Materials"],"abstract":"<jats:p>Due to environmental concerns, the search for sustainable construction solutions has been increasing over the years. This global concern is creating a trend in the use of recycled aggregates resulting from construction and demolition wastes from different sources. In addition to their physical and mechanical properties, it is important to analyse their ecotoxicological risk to determine whether their leachates might be an issue. To assess ecotoxicity, biological tests should be performed for different trophic levels. This type of test is expensive and needs a high level of expertise, which leads to a lack of studies on recycled aggregates including ecotoxicity analysis. This paper presents a set of predictive ecotoxicity results based on the published studies on recycled aggregates. These results are the outcome of applying an innovative methodology previously developed and validated by the authors aiming to foresee the ecotoxicological fate of building materials\u2019 constituents and products. The application of this methodology enables the classification of a recycled aggregate product as safe or unsafe in terms of ecotoxicity risk, while keeping biological testing to a minimum.<\/jats:p>","DOI":"10.3390\/ma15103510","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T08:37:02Z","timestamp":1652431022000},"page":"3510","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ecotoxicity of Recycled Aggregates: Application of a Prediction Methodology"],"prefix":"10.3390","volume":"15","author":[{"given":"Margarida B.","family":"Maia","sequence":"first","affiliation":[{"name":"Civil Engineering Research and Innovation for Sustainability (CERIS), Department of Civil Engineering, Architecture and Georresources, Instituto Superior T\u00e9cnico, University of Lisbon, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6766-2736","authenticated-orcid":false,"given":"Jorge","family":"de Brito","sequence":"additional","affiliation":[{"name":"Civil Engineering Research and Innovation for Sustainability (CERIS), Department of Civil Engineering, Architecture and Georresources, Instituto Superior T\u00e9cnico, University of Lisbon, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7116-7543","authenticated-orcid":false,"given":"Isabel M.","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Materials, Laborat\u00f3rio Nacional de Engenharia Civil (LNEC), Av. do Brasil 101, 1700-066 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3330-2000","authenticated-orcid":false,"given":"Jos\u00e9 D.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Civil Engineering Research and Innovation for Sustainability (CERIS), Department of Civil Engineering, Architecture and Georresources, Instituto Superior T\u00e9cnico, University of Lisbon, 1049-001 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,13]]},"reference":[{"key":"ref_1","unstructured":"(2021, December 02). Generation of Waste by Waste Category, Hazardousness and NACE Rev. 2 Activity. Available online: https:\/\/ec.europa.eu\/eurostat\/databrowser\/view\/ENV_WASGEN__custom_1692510\/default\/table?lang=en."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"122913","DOI":"10.1016\/j.jclepro.2020.122913","article-title":"Mechanical behaviour of structural concrete with ground recycled concrete cement and mixed recycled aggregate","volume":"275","author":"Cantero","year":"2020","journal-title":"J. Clean. Prod."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Rahhal, V.F., Trezza, M.A., Tironi, A., Castellano, C.C., Pavl\u00edkov\u00e1, M., Pokorn\u00fd, J., Irassar, E.F., Jankovsk\u00fd, O., and Pavl\u00edk, Z. (2019). Complex Characterization and Behavior of Waste Fired Brick Powder-Portland Cement System. Materials, 12.","DOI":"10.3390\/ma12101650"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.wasman.2015.12.021","article-title":"Use of recycled aggregates from construction and demolition waste in geotechnical applications: A literature review","volume":"49","author":"Cardoso","year":"2016","journal-title":"Waste Manag."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.resconrec.2011.11.003","article-title":"Construction of road sections using mixed recycled aggregates treated with cement in Malaga, Spain","volume":"58","author":"Agrela","year":"2012","journal-title":"Resour. Conserv. Recycl."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"116844","DOI":"10.1016\/j.conbuildmat.2019.116844","article-title":"Rendering mortars with incorporation of very fine aggregates from construction and demolition waste","volume":"229","author":"Jesus","year":"2019","journal-title":"Constr. Build. Mater."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.conbuildmat.2014.08.080","article-title":"Properties of masonry mortars manufactured with fine recycled concrete aggregates","volume":"71","author":"Ledesma","year":"2014","journal-title":"Constr. Build. Mater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.conbuildmat.2014.04.117","article-title":"Properties and composition of recycled aggregates from construction and demolition waste suitable for concrete production","volume":"65","author":"Silva","year":"2014","journal-title":"Constr. Build. Mater."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.conbuildmat.2018.12.200","article-title":"Experimental investigation on the variability of the main mechanical properties of concrete produced with coarse recycled concrete aggregates","volume":"201","author":"Pacheco","year":"2019","journal-title":"Constr. Build. Mater."},{"key":"ref_10","unstructured":"Cenci, C.S., Tadeu, A., De Brito, J., and Veiga, R. (2021, January 12\u201315). A brief framework of construction and demolition waste composition in Portugal within the European context. Proceedings of the CEES 2021\u2013International Conference on Construction, Energy, Environmental & Sustainability, Coimbra, Portugal."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"36014","DOI":"10.1007\/s11356-017-1017-8","article-title":"Upscaling the pollutant emission from mixed recycled aggregates under compaction for civil applications","volume":"25","author":"Ayuso","year":"2018","journal-title":"Environ. Sci Pollut. Res."},{"key":"ref_12","unstructured":"(2021, October 19). Directive 2013\/39\/EU of the European Parliament and of the Council of 12 August 2013 amending Directives 2000\/60\/EC and 2008\/105\/EC as Regards Priority Substances in the Field of Water Policy Text with EEA Relevance. Available online: http:\/\/eur-lex.europa.eu\/."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0166-1116(97)80204-X","article-title":"Integration of testing protocols for evaluation of contaminant release from monolithic and granular wastes","volume":"71","author":"Kosson","year":"1997","journal-title":"Stud. Environ. Sci"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0147-6513(77)90033-1","article-title":"Ecotoxicology: Objectives, principles and perspectives","volume":"1","author":"Truhaut","year":"1977","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_15","unstructured":"(2017). Construction Products-Assessment of Release of Dangerous Substances-Guidance on the Use of Ecotoxicity Tests Applied to Construction Products (Standard No. CEN\/TR 17105)."},{"key":"ref_16","unstructured":"(2007). Water Quality-Determination of the Inhibitory Effect of Water Samples on the Light Emission of Vibrio Fischeri (Luminescent Bacteria Test)-Part 3: Method Using Freeze-Dried Bacteria (Standard No. ISO 11348-3)."},{"key":"ref_17","unstructured":"(2008). Water Quality-Fresh Water Algal Growth Inhibition Test with Unicellular Green Algae (Standard No. ISO 8692)."},{"key":"ref_18","unstructured":"(2008). Water Quality-Determination of the Inhibition of the Mobility of Daphnia Magna Straus (Cladocera, Crustacea)-Acute Toxicity Test (Standard No. ISO 6341)."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"12983","DOI":"10.1007\/s11356-016-6456-0","article-title":"Effects of extraction methods and factors on leaching of metals from recycled concrete aggregates","volume":"23","author":"Bestgen","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_20","first-page":"3729","article-title":"pH-dependent leaching of trace elements from recycled concrete aggregate","volume":"2012","author":"Chen","year":"2012","journal-title":"Geo Congr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"35565","DOI":"10.1007\/s11356-019-04925-5","article-title":"Long-term leaching and mechanical behaviour at recycled aggregate with different gypsum contents","volume":"26","author":"Barbudo","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.wasman.2015.07.051","article-title":"Analysis of chromium and sulphate origins in construction recycled materials based on leaching test results","volume":"46","author":"Ayuso","year":"2015","journal-title":"Waste Manag."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.wasman.2012.02.005","article-title":"Correlation analysis between sulphate content and leaching of sulphates in recycled aggregates from construction and demolition wastes","volume":"32","author":"Barbudo","year":"2012","journal-title":"Waste Manag."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1080\/14680629.2019.1701539","article-title":"Feasibility study of milled and crushed reclaimed asphalt pavement for application in unbound granular layers","volume":"22","author":"Freire","year":"2021","journal-title":"Road Mater. Pavement Des."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Rodrigues, P., Silvestre, J.D., Flores-Colen, I., Viegas, C.A., Ahmed, H.H., Kurda, R., and de Brito, J. (2020). Evaluation of the ecotoxicological potential of fly ash and recycled concrete aggregates use in concrete. Appl. Sci., 10.","DOI":"10.3390\/app10010351"},{"key":"ref_26","unstructured":"Freire, A.C., and Antunes, M.L. (2009, January 1\u201330). Recycled aggregates characterization according to the European Standards. Proceedings of the Waste Recovery in Geotechnical Works-Characterization and Measures for the Development of the Sector, Aveiro, Portugal. (In Portuguese)."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"R\u00f6mbke, J. (2017, January 2\u20136). Testing of 24 potentially hazardous wastes using 6 ecotoxicological tests. Proceedings of the Sardinia 2017\/Sixteenth International Waste Management and Landfill Symposium, Cagliari, Italy.","DOI":"10.31025\/2611-4135\/2018.13745"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1007\/s00244-010-9631-1","article-title":"A comparative toxicity assessment of materials used in aquatic construction","volume":"61","author":"Lalonde","year":"2011","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1007\/s12649-018-0382-y","article-title":"Recycling wastes in concrete production: Performance and eco-toxicity assessment","volume":"11","author":"Silva","year":"2020","journal-title":"Waste Biomass Valoriz."},{"key":"ref_30","first-page":"41","article-title":"Evaluation of daphnia magna for the ecotoxicity assessment of alkali leachate from concrete","volume":"26","author":"Choi","year":"2013","journal-title":"Int. J. Ind."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"012023","DOI":"10.1088\/1755-1315\/290\/1\/012023","article-title":"Environmental impact of concrete and concrete-based construction waste leachates","volume":"290","author":"Sackey","year":"2019","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Mariakov\u00e1, D., Anna Mocov\u00e1, K., Fo\u0159tov\u00e1, K., Ryparov\u00e1, P., Pe\u0161ta, J., and Pavl\u016f, T. (2021). Ecotoxicity and essential properties of fine-recycled aggregate. Materials, 14.","DOI":"10.3390\/ma14020463"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Rodrigues, P., Silvestre, J.D., Flores-Colen, I., Viegas, C.A., de Brito, J., Kurad, R., and Demertzi, M. (2017). Methodology for the assessment of the ecotoxicological potential of construction materials. Materials, 10.","DOI":"10.3390\/ma10060649"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"042067","DOI":"10.1088\/1755-1315\/588\/4\/042067","article-title":"Toxicity of cement-based materials","volume":"588","author":"Maia","year":"2020","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"124362","DOI":"10.1016\/j.jclepro.2020.124362","article-title":"Environmental assessment, mechanical behavior and new leaching impact proposal of mixed recycled aggregates to be used in road construction","volume":"280","author":"Agrela","year":"2020","journal-title":"J. Clean. Prod."},{"key":"ref_36","unstructured":"(2002). Characterisation of Waste-Leaching-Compliance Test for Leaching of Granular Waste Materials and Sludges-Part 3: Two Stage Batch Test at a Liquid to Solid Ratio of 2 L\/kg and 8 L\/kg for Materials with a High Solid Content and with a Particle Size below 4 mm (without or with size reduction) (Standard No. CEN, EN 12457-3:2002)."},{"key":"ref_37","unstructured":"(2002). Characterisation of Waste-Leaching-Compliance Test for Leaching of Granular Waste Materials and Sludges-Part 4: One Stage Batch Test at a Liquid to Solid Ratio of 10 L\/kg for Materials with Particle Size below 10 mm (without or with Size Reduction) (Standard No. CEN, EN 12457-4: 2002)."},{"key":"ref_38","unstructured":"(2007). Water Quality-Determination of Dissolved Anions by Liquid Chromatography of Ions-Part 1: Determination of Bromide, Chloride, Fluoride, Nitrate, Nitrite, Phosphate and Sulfate (Standard No. ISO 10304-1:2009)."},{"key":"ref_39","unstructured":"(2017). Solids-Standard Methods for the Examination of Water and Wastewater (Standard No. SMEWW 2540:2017)."},{"key":"ref_40","unstructured":"(2017). Total Organic Carbon (TOC)-Standard Methods for the Examination of Water and Wastewater (Standard No. SMEWW 5310:2017)."},{"key":"ref_41","unstructured":"(1993). F\u00edler Calc\u00e1rio para Bet\u00f5es. Determina\u00e7\u00e3o do Teor de Carbono Org\u00e2nico Total (TOC) (Standard No. E 386:1993). (In Portuguese)."},{"key":"ref_42","unstructured":"(2021, October 18). Council Decision 2003\/33\/EC, of 19 December 2002 establishing criteria and procedures for the acceptance of waste at landfills pursuant to Article 16 of and Annex II to Directive 1999\/31\/EC. Available online: http:\/\/eur-lex.europa.eu\/."},{"key":"ref_43","unstructured":"(2021, October 18). Regulation (EC) No 1272\/2008, of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67\/548\/EEC and 1999\/45\/EC, and amending Regulation (EC) No 1907\/2006. Available online: http:\/\/eur-lex.europa.eu\/."},{"key":"ref_44","unstructured":"(2021, December 05). Explore Chemistry, Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/."},{"key":"ref_45","unstructured":"(2021, December 05). European Chemicals Agency. Available online: https:\/\/echa.europa.eu\/."},{"key":"ref_46","unstructured":"(2021, October 12). Ecotox Knowledgebase, Available online: https:\/\/cfpub.epa.gov\/ecotox\/search.cfm."}],"container-title":["Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1944\/15\/10\/3510\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:10:20Z","timestamp":1760137820000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1944\/15\/10\/3510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,13]]},"references-count":46,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["ma15103510"],"URL":"https:\/\/doi.org\/10.3390\/ma15103510","relation":{},"ISSN":["1996-1944"],"issn-type":[{"type":"electronic","value":"1996-1944"}],"subject":[],"published":{"date-parts":[[2022,5,13]]}}}