{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:31:59Z","timestamp":1760243519100,"version":"build-2065373602"},"reference-count":24,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2013,8,13]],"date-time":"2013-08-13T00:00:00Z","timestamp":1376352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The phylum Cnidaria is an ancient group of venomous animals, specialized in the production and delivery of toxins. Many species belonging to the class Anthozoa have been studied and their venoms often contain a group of peptides, less than 10 kDa, that act upon ion channels. These peptides and their targets interact with high affinity producing neurotoxic and cardiotoxic effects, and even death, depending on the dose and the administration pathway. Zoanthiniaria is an order of the Subclass Hexacorallia, class Anthozoa, and unlike sea anemone (order Actiniaria), neither its diversity of toxins nor the in vivo effects of the venoms has been exhaustively explored. In this study we assessed some toxicological tests on mice with a low molecular weight fraction obtained by gel filtration in Sephadex G-50 from Zoanthus sociatus crude extract. The gel filtration chromatogram at 280 nm revealed two major peaks, the highest absorbance corresponding to the low molecular weight fraction. The toxicological effects seem to be mostly autonomic and cardiotoxic, causing death in a dose dependent manner with a LD50 of  792 \u03bcg\/kg. Moreover, at a dose of 600 \u03bcg\/kg the active fraction accelerated the  KCl-induced lethality in mice.<\/jats:p>","DOI":"10.3390\/md11082873","type":"journal-article","created":{"date-parts":[[2013,8,13]],"date-time":"2013-08-13T11:55:07Z","timestamp":1376394907000},"page":"2873-2881","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Insights into the Toxicological Properties of a Low Molecular Weight Fraction from Zoanthus sociatus (Cnidaria)"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5211-972X","authenticated-orcid":false,"given":"Dany","family":"Dom\u00ednguez-P\u00e9rez","sequence":"first","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"},{"name":"Biology Department, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"given":"Carlos","family":"Diaz-Garcia","sequence":"additional","affiliation":[{"name":"PhD Program in Biological Sciences, National Autonomous University of Mexico, Institute of Cellular Physiology, Mexico DF CP04510, Mexico"}]},{"given":"Neivys","family":"Garc\u00eda-Delgado","sequence":"additional","affiliation":[{"name":"PhD Program in Biomedical Sciences, National Autonomous University of Mexico, Institute of Cellular Physiology, Mexico DF CP04510, Mexico"}]},{"given":"Yusvel","family":"Sierra-G\u00f3mez","sequence":"additional","affiliation":[{"name":"PhD Program in Biomedical Sciences, National Autonomous University of Mexico, Institute of Cellular Physiology, Mexico DF CP04510, Mexico"}]},{"given":"Olga","family":"Casta\u00f1eda","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of La Habana, 25 St 455, La Habana CP 10400, Cuba"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-1732","authenticated-orcid":false,"given":"Agostinho","family":"Antunes","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"},{"name":"Biology Department, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2013,8,13]]},"reference":[{"key":"ref_1","unstructured":"World Register of Marine Species. Available online:http:\/\/www.marinespecies.org\/aphia.php?p=taxdetails&id=1267."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.11646\/zootaxa.1668.1.11","article-title":"The phylum Cnidaria: A review of phylogenetic patterns and diversity 300 years after Linnaeus","volume":"1668","author":"Daly","year":"2007","journal-title":"Zootaxa"},{"key":"ref_3","unstructured":"Weill, R. (1934). Contribution a L\u2019\u00e9tude des Cnidaires et de Leurs N\u00e9matocystes: Recherches sur les N\u00e9matocystes (Morphologie, Physiologie, D\u00e9veloppement) la Valeur Taxonomique du Cnidome, Laboratoire D\u2019\u00e9volution des \u00catres Organis\u00e9s."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0022-5320(84)80075-1","article-title":"Ultrastructure and sulfur cytochemistry of nematocyst development in catch tentacles of the sea anemone Haliplanella luciae (Cnidaria: Anthozoa)","volume":"87","author":"Watson","year":"1984","journal-title":"J. Ultrastruct. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/j.toxicon.2009.02.024","article-title":"Structural diversity, systematics, and evolution of cnidae","volume":"54","author":"Fautin","year":"2009","journal-title":"Toxicon"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/j.toxicon.2009.06.031","article-title":"The phylum Cnidaria and investigations of its toxins and venoms until 1990","volume":"54","author":"Turk","year":"2009","journal-title":"Toxicon"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.3390\/md10081812","article-title":"Sea anemone (Cnidaria, Anthozoa, Actiniaria) toxins: An overview","volume":"10","author":"Vasconcelos","year":"2012","journal-title":"Mar. Drugs"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Fattorusso, E., Gerwick, W.H., and Taglialatela-Scafati, O. (2012). Handbook of Marine Natural Products, Springer.","DOI":"10.1007\/978-90-481-3834-0"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.toxicon.2012.05.020","article-title":"Development of a rational nomenclature for naming peptide and protein toxins from sea anemones","volume":"60","author":"Oliveira","year":"2012","journal-title":"Toxicon"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10126-005-5093-2","article-title":"Peptide toxins in sea anemones: Structural and functional aspects","volume":"8","author":"Honma","year":"2006","journal-title":"Mar. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1042\/BJ20121623","article-title":"AdE-1, a new inotropic Na+ channel toxin from Aiptasia diaphana, is similar to, yet distinct from, known anemone Na+ channel toxins","volume":"451","author":"Nesher","year":"2013","journal-title":"Biochem. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"655","DOI":"10.3390\/md11030655","article-title":"Biochemical and electrophysiological characterization of two sea anemone type 1 potassium toxins from a geographically distant population of Bunodosoma caissarum","volume":"11","author":"Orts","year":"2013","journal-title":"Mar. Drugs"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/0041-0101(95)00079-2","article-title":"Studies on the origin and distribution of palytoxin in a Caribbean coral reef","volume":"33","author":"Gleibs","year":"1995","journal-title":"Toxicon"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1016\/S0968-0896(03)00107-X","article-title":"Evaluation of the effects of several zoanthamine-type alkaloids on the aggregation of human platelets","volume":"11","author":"Villar","year":"2003","journal-title":"Bioorg. Med. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1007\/s00436-004-1124-6","article-title":"Antifilarial activity of Zoanthus species (phylum Coelenterata, class Anthzoa) against human lymphatic filaria, Brugia malayi","volume":"93","author":"Lakshmi","year":"2004","journal-title":"Parasitol. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.toxicon.2011.11.019","article-title":"Low molecular weight compounds from Zoanthus sociatus impair insulin secretion via Ca+2 influx blockade and cause glucose intolerance in vivo","volume":"59","author":"Varela","year":"2012","journal-title":"Toxicon"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.toxicon.2013.02.015","article-title":"Arrhythmogenic effect of a crude extract from sea anemone Condylactis gigantea: Possible involvement of rErg1 channels","volume":"67","author":"Santos","year":"2013","journal-title":"Toxicon"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.1742-7843.2007.00038.x","article-title":"Assessment of acute respiratory and cardiovascular toxicity of casiopeinas in anaesthetized dogs","volume":"101","author":"Sumano","year":"2007","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1351\/pac198254101981","article-title":"Biological active compounds from Coelenterates","volume":"54","author":"Beress","year":"1981","journal-title":"Pure Appl. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.yjmcc.2009.07.026","article-title":"l-Type Ca(2+) current in ventricular cardiomyocytes","volume":"48","author":"Benitah","year":"2009","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"S661","DOI":"10.1016\/S0003-4975(02)04688-X","article-title":"Mechanisms and alternative methods of achieving cardiac arrest","volume":"75","author":"Chambers","year":"2003","journal-title":"Ann. Thorac. Surg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1055\/s-2002-20585","article-title":"Calcium and beta receptor antagonist overdose: A review and update of pharmacological principles and management","volume":"23","author":"Newton","year":"2002","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_23","first-page":"241","article-title":"Calcium channel blockers, beta-blockers and digitalis poisoning: Management in the emergency room","volume":"9","author":"Ojetti","year":"2005","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1097\/01.CCM.0000278916.04569.23","article-title":"Assessment of hyperglycemia after calcium channel blocker overdoses involving diltiazem or verapamil","volume":"35","author":"Levine","year":"2007","journal-title":"Crit. Care Med."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/11\/8\/2873\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:48:36Z","timestamp":1760219316000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/11\/8\/2873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,13]]},"references-count":24,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2013,8]]}},"alternative-id":["md11082873"],"URL":"https:\/\/doi.org\/10.3390\/md11082873","relation":{},"ISSN":["1660-3397"],"issn-type":[{"type":"electronic","value":"1660-3397"}],"subject":[],"published":{"date-parts":[[2013,8,13]]}}}