{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T05:58:46Z","timestamp":1770703126246,"version":"3.49.0"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2016,8,31]],"date-time":"2016-08-31T00:00:00Z","timestamp":1472601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund (ERDF)","award":["NORTE-01-0145-FEDER-000035"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000035"]}]},{"name":"FCT Foundation for Science and Technology","award":["SFRH\/BPD\/112287\/2015"],"award-info":[{"award-number":["SFRH\/BPD\/112287\/2015"]}]},{"name":"FCT Foundation for Science and Technology","award":["IF\/01358\/2014"],"award-info":[{"award-number":["IF\/01358\/2014"]}]},{"name":"FCT Foundation for Science and Technology","award":["UID\/Multi\/04423\/2013"],"award-info":[{"award-number":["UID\/Multi\/04423\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Background: Hierridin B was isolated from a marine cyanobacterium Cyanobium sp. strain and induced cytotoxicity selectively in HT-29 adenocarcinoma cells. The underlying molecular mechanism was not yet elucidated. Methods: HT-29 cells were exposed to the IC50 concentration of hierridin B (100.2 \u03bcM) for 48 h. Non-targeted proteomics was performed using 2D gel electrophoresis and MALDI-TOF\/TOF mass spectrometry. The mRNA expression of apoptotic and cell cycle genes were analyzed by real-time PCR. Automated quantification of 160 cytoplasm and mitochondrial parameter was done by fluorescence microscopy using CellProfiler software. Results: Proteomics identified 21 significant different proteins, which belonged to protein folding\/synthesis and cell structure amongst others. Increase of VDAC1 protein responsible for formation of mitochondrial channels was confirmed by mRNA expression. A 10-fold decrease of cytoskeleton proteins (STMN1, TBCA) provided a link to alterations of the cell cycle. CCNB1 and CCNE mRNA were decreased two-fold, and P21CIP increased 10-fold, indicative of cell cycle arrest. Morphological analysis of mitochondrial parameter confirmed a reduced mitochondrial activity. Conclusion: Hierridin B is a potential anticancer compound that targets mitochondrial activity and function.<\/jats:p>","DOI":"10.3390\/md14090158","type":"journal-article","created":{"date-parts":[[2016,8,31]],"date-time":"2016-08-31T13:11:45Z","timestamp":1472649105000},"page":"158","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Hierridin B Isolated from a Marine Cyanobacterium Alters VDAC1, Mitochondrial Activity, and Cell Cycle Genes on HT-29 Colon Adenocarcinoma Cells"],"prefix":"10.3390","volume":"14","author":[{"given":"Sara","family":"Freitas","sequence":"first","affiliation":[{"name":"Interdisciplinary Center of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"}]},{"given":"Ros\u00e1rio","family":"Martins","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"},{"name":"Health and Environmental Research Center (CISA), School of Apllied Health Sciences of Porto, Polytechnic Porto, Rua Valente Perfeito 322, 4400-330 Vila Nova de Gaia, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"},{"name":"Institute for Molecular and Cell Biology (IBMC), University of Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9147-8621","authenticated-orcid":false,"given":"Margarida","family":"Costa","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"}]},{"given":"Pedro","family":"Le\u00e3o","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3636-5805","authenticated-orcid":false,"given":"Rui","family":"Vitorino","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Institute of Biomedicine\u2014iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal"},{"name":"Department of Physiology and Cardiothoracic Surgery, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7476-9195","authenticated-orcid":false,"given":"Ralph","family":"Urbatzka","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2016,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zugazagoitia, J., Guedes, C., Ponce, S., Ferrer, I., Molina-Pinelo, S., and Paz-Ares, L. 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