{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:18:37Z","timestamp":1762867117825,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,4,9]],"date-time":"2017-04-09T00:00:00Z","timestamp":1491696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Energy-Joint Genome Institute (DOE-JGI)","award":["CSP proposal ID 328, project IDs 403959, 404206, 404777- 404782, 404786, 404788- 404801"],"award-info":[{"award-number":["CSP proposal ID 328, project IDs 403959, 404206, 404777- 404782, 404786, 404788- 404801"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","award":["PIP N\u00ba 11220130100174","PIP N\u00b0 11220110101156","11220150100934CO"],"award-info":[{"award-number":["PIP N\u00ba 11220130100174","PIP N\u00b0 11220110101156","11220150100934CO"]}]},{"name":"National Agency for the Promotion of Science and Technology of Argentina","award":["PICT2015 N\u00ba 2102"],"award-info":[{"award-number":["PICT2015 N\u00ba 2102"]}]},{"DOI":"10.13039\/501100008793","name":"University of Rosario","doi-asserted-by":"publisher","award":["UNR BIO287, BIO451"],"award-info":[{"award-number":["UNR BIO287, BIO451"]}],"id":[{"id":"10.13039\/501100008793","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","award":["2014-2017 20020130100569BA"],"award-info":[{"award-number":["2014-2017 20020130100569BA"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Commission through the Marie Curie Action IRSES IMCONet","award":["318718"],"award-info":[{"award-number":["318718"]}]},{"name":"Argentinean Antarctic Institute and ANPCyT","award":["PICTO 2010 N\u00b0 0124"],"award-info":[{"award-number":["PICTO 2010 N\u00b0 0124"]}]},{"DOI":"10.13039\/100011661","name":"Pacific Northwest National Laboratory","doi-asserted-by":"publisher","award":["DE-AC05-76RLO1830"],"award-info":[{"award-number":["DE-AC05-76RLO1830"]}],"id":[{"id":"10.13039\/100011661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["228107"],"award-info":[{"award-number":["228107"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The goal of this work was to identify sequences encoding monooxygenase biocatalysts with novel features by in silico mining an assembled metagenomic dataset of polar and subpolar marine sediments. The targeted enzyme sequences were Baeyer\u2013Villiger and bacterial cytochrome P450 monooxygenases (CYP153). These enzymes have wide-ranging applications, from the synthesis of steroids, antibiotics, mycotoxins and pheromones to the synthesis of monomers for polymerization and anticancer precursors, due to their extraordinary enantio-, regio-, and chemo- selectivity that are valuable features for organic synthesis. Phylogenetic analyses were used to select the most divergent sequences affiliated to these enzyme families among the 264 putative monooxygenases recovered from the ~14 million protein-coding sequences in the assembled metagenome dataset. Three-dimensional structure modeling and docking analysis suggested features useful in biotechnological applications in five metagenomic sequences, such as wide substrate range, novel substrate specificity or regioselectivity. Further analysis revealed structural features associated with psychrophilic enzymes, such as broader substrate accessibility, larger catalytic pockets or low domain interactions, suggesting that they could be applied in biooxidations at room or low temperatures, saving costs inherent to energy consumption. This work allowed the identification of putative enzyme candidates with promising features from metagenomes, providing a suitable starting point for further developments.<\/jats:p>","DOI":"10.3390\/md15040114","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T02:39:17Z","timestamp":1492051157000},"page":"114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Prospecting Biotechnologically-Relevant Monooxygenases from Cold Sediment Metagenomes: An In Silico Approach"],"prefix":"10.3390","volume":"15","author":[{"given":"Mat\u00edas","family":"Musumeci","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Ambiental, Centro para el Estudio de Sistemas Marinos, CONICET, Puerto Madryn, Chubut U9120ACD, Argentina"}]},{"given":"Mariana","family":"Lozada","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Ambiental, Centro para el Estudio de Sistemas Marinos, CONICET, Puerto Madryn, Chubut U9120ACD, Argentina"}]},{"given":"Daniela","family":"Rial","sequence":"additional","affiliation":[{"name":"\u00c1rea Biolog\u00eda Molecular, Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, CONICET, Suipacha 531 S2002LRK Rosario, Argentina"}]},{"given":"Walter","family":"Mac Cormack","sequence":"additional","affiliation":[{"name":"Instituto Ant\u00e1rtico Argentino, Ciudad Aut\u00f3noma de Buenos Aires C1010AAZ, Argentina"},{"name":"Instituto de Nanobiotecnolog\u00eda (NANOBIOTEC), CONICET\u2014Universidad de Buenos Aires, Ciudad Aut\u00f3noma de Buenos Aires C1113AAD, Argentina"}]},{"given":"Janet","family":"Jansson","sequence":"additional","affiliation":[{"name":"Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA"}]},{"given":"Sara","family":"Sj\u00f6ling","sequence":"additional","affiliation":[{"name":"School of Natural Sciences and Environmental Studies, S\u00f6dert\u00f6rn University, 141 89 Huddinge, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6598-0818","authenticated-orcid":false,"given":"JoLynn","family":"Carroll","sequence":"additional","affiliation":[{"name":"Akvaplan-niva, Fram\u2014High North Research Centre for Climate and the Environment, NO-9296 Troms\u00f8, Norway"},{"name":"ARCEx\u2014Research Centre for Arctic Petroleum Exploration, Department of Geosciences, UiT The Arctic University of Norway, N-9037 Troms\u00f8, Norway"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8075-2311","authenticated-orcid":false,"given":"Hebe","family":"Dionisi","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Ambiental, Centro para el Estudio de Sistemas Marinos, CONICET, Puerto Madryn, Chubut U9120ACD, Argentina"}]}],"member":"1968","published-online":{"date-parts":[[2017,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"134","DOI":"10.2174\/187220812801784696","article-title":"Some enzymes in marine environment: Prospective applications found in patent literature","volume":"6","author":"Trincone","year":"2012","journal-title":"Recent Patents Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kim, S.K. 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