{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T10:28:38Z","timestamp":1775644118175,"version":"3.50.1"},"reference-count":78,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T00:00:00Z","timestamp":1507852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Food protein-derived hydrolysates with multi-bioactivities such as antihypertensive and antioxidant properties have recently received special attention since both activities can play significant roles in preventing cardiovascular diseases. This study reports, for the first time, the angiotensin I-converting enzyme (ACE)-inhibition and antioxidant properties of ultrafiltrate fractions (UF) with different molecular weight ranges (&lt;1, 1\u20133 and \u22653 kDa) obtained from Fucus spiralis protein hydrolysate (FSPH) digested with cellulase\u2013bromelain. The amino acids profile, recovery yield, protein, peptide and total phenolic contents of these FSPH-UF, and the in vitro digestibility of F. spiralis crude protein were also investigated. FSPH-UF \u22653 kDa presented remarkably higher ACE-inhibition, yield, peptide and polyphenolic (phlorotannins) contents. Antioxidant analysis showed that FSPH-UF &lt;1 kDa and \u22653 kDa exhibited significantly higher scavenging of 2,2-diphenyl-1-picrylhydrazyl radical and ferrous ion-chelating (FIC) activity. FSPH-UF \u22653 kDa had also notably higher ferric reducing antioxidant power (FRAP). Strong correlations were observed between ACE-inhibition and antioxidant activities (FIC and FRAP). The results suggest that ACE-inhibition and antioxidant properties of FSPH-UF may be due to the bioactive peptides and polyphenols released during the enzymatic hydrolysis. In conclusion, this study shows the potential use of defined size FSPH-UF for the prevention\/treatment of hypertension and\/or oxidative stress-related diseases.<\/jats:p>","DOI":"10.3390\/md15100311","type":"journal-article","created":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T11:34:09Z","timestamp":1507894449000},"page":"311","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity, Antioxidant Properties, Phenolic Content and Amino Acid Profiles of Fucus spiralis L. Protein Hydrolysate Fractions"],"prefix":"10.3390","volume":"15","author":[{"given":"Lisete","family":"Paiva","sequence":"first","affiliation":[{"name":"Biotechnology Centre of Azores (CBA), University of Azores, 9501-801 Ponta Delgada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6736-8729","authenticated-orcid":false,"given":"Elisabete","family":"Lima","sequence":"additional","affiliation":[{"name":"Biotechnology Centre of Azores (CBA), University of Azores, 9501-801 Ponta Delgada, Portugal"},{"name":"Research Center for Agricultural Technology (CITA-A), University of Azores, 9501-801 Ponta Delgada, Portugal"}]},{"given":"Ana","family":"Neto","sequence":"additional","affiliation":[{"name":"Azorean Biodiversity Group, Centre for Ecology, Evolution and Environmental Changes (CE3C), Department of Biology, University of Azores, 9501-801 Ponta Delgada, Portugal"}]},{"given":"Jos\u00e9","family":"Baptista","sequence":"additional","affiliation":[{"name":"Biotechnology Centre of Azores (CBA), University of Azores, 9501-801 Ponta Delgada, Portugal"},{"name":"Research Center for Agricultural Technology (CITA-A), University of Azores, 9501-801 Ponta Delgada, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2017,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.3390\/md9061056","article-title":"Marine bioactives as functional food ingredients: Potential to reduce the incidence of chronic diseases","volume":"9","author":"Lordan","year":"2011","journal-title":"Mar. 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