{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:41:54Z","timestamp":1774690914200,"version":"3.50.1"},"reference-count":199,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T00:00:00Z","timestamp":1534204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"H2020-TWINN-2015","award":["BLUEandGREEN  (grant  692419)"],"award-info":[{"award-number":["BLUEandGREEN  (grant  692419)"]}]},{"DOI":"10.13039\/501100001871","name":"FCT","doi-asserted-by":"publisher","award":["UID\/Multi\/04423\/2013"],"award-info":[{"award-number":["UID\/Multi\/04423\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Interreg Atlantic Area","award":["BLUEHUMAN (EAPA_151\/2016)"],"award-info":[{"award-number":["BLUEHUMAN (EAPA_151\/2016)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The increase of many deadly diseases like infections by multidrug-resistant bacteria implies re-inventing the wheel on drug discovery. A better comprehension of the metabolisms and regulation of diseases, the increase in knowledge based on the study of disease-born microorganisms\u2019 genomes, the development of more representative disease models and improvement of techniques, technologies, and computation applied to biology are advances that will foster drug discovery in upcoming years. In this paper, several aspects of current methodologies for drug discovery of antibacterial and antifungals, anti-tropical diseases, antibiofilm and antiquorum sensing, anticancer and neuroprotectors are considered. For drug discovery, two different complementary approaches can be applied: classical pharmacology, also known as phenotypic drug discovery, which is the historical basis of drug discovery, and reverse pharmacology, also designated target-based drug discovery. Screening methods based on phenotypic drug discovery have been used to discover new natural products mainly from terrestrial origin. Examples of the discovery of marine natural products are provided. A section on future trends provides a comprehensive overview on recent advances that will foster the pharmaceutical industry.<\/jats:p>","DOI":"10.3390\/md16080279","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T10:31:16Z","timestamp":1534242676000},"page":"279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":112,"title":["Current Screening Methodologies in Drug Discovery for Selected Human Diseases"],"prefix":"10.3390","volume":"16","author":[{"given":"Olga Maria","family":"Lage","sequence":"first","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre s\/n\u00ba 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR\u2013Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental\u2013Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Mar\u00eda C.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, 18016 Granada, Spain"}]},{"given":"Rita","family":"Calisto","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre s\/n\u00ba 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR\u2013Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental\u2013Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Eduarda","family":"Almeida","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre s\/n\u00ba 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR\u2013Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental\u2013Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre s\/n\u00ba 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR\u2013Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental\u2013Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Francisca","family":"Vicente","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Parque Tecnol\u00f3gico de Ciencias de la Salud, 18016 Granada, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/dta.301","article-title":"Early drug discovery and the rise of pharmaceutical chemistry","volume":"3","year":"2011","journal-title":"Drug Test. 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