{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T05:50:27Z","timestamp":1773726627287,"version":"3.50.1"},"reference-count":73,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,4,22]],"date-time":"2019-04-22T00:00:00Z","timestamp":1555891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Chitin was extracted from Polybius henslowii, a swimming crab, captured in large quantities throughout the Portuguese coast by purse seine vessels as bycatch. After standard chitin extraction procedures, water-soluble chitosan products were obtained via two different methods: (1) N-acetylation with the addition of acetic anhydride and (2) a reaction with hydrogen peroxide. The chemical structure and molecular weight of chitosan derivatives, water-soluble chitosan (WSC) and chitooligosaccharides (COS), were confirmed by Fourier Transform Infrared Spectroscopy (FT-IR) and gel permeation chromatography (GPC). Antioxidant and metal chelation activities were evaluated, and the growth inhibition capacity was tested on four phytopatogens. The chitooligosaccharides from pereopods (pCOS) and shell body parts (sCOS) inhibited all fungal species tested, particularly Cryphonectria parasitica with 84.7% and 85.5%, respectively. Both radical scavenging and antifungal activities proved to be dose-dependent. Chitooligosaccharides with a low molecular weight (2.7, 7.4, and 10.4 Kg\u00b7mol\u22121) showed the highest activity among all properties tested. These results suggested that chitosan derivatives from P. henslowii raw material could potentially be used against phytopathogens or as ingredient in cosmetics and other products related to oxidative stress.<\/jats:p>","DOI":"10.3390\/md17040239","type":"journal-article","created":{"date-parts":[[2019,4,22]],"date-time":"2019-04-22T11:02:53Z","timestamp":1555930973000},"page":"239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":155,"title":["Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources"],"prefix":"10.3390","volume":"17","author":[{"given":"Francisco","family":"Avelelas","sequence":"first","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Instituto Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"given":"Andr\u00e9","family":"Horta","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Instituto Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5697-4363","authenticated-orcid":false,"given":"Lu\u00eds F.V.","family":"Pinto","sequence":"additional","affiliation":[{"name":"BioCeramed, S.A., Rua Jos\u00e9 Gomes Ferreira n\u00ba 1 - Armaz\u00e9m D 2660-360 S\u00e3o Juli\u00e3o do Tojal, Portugal"},{"name":"CENIMAT\/I3N, Departamento de Ci\u00eancia dos Materiais, Faculdade de Ci\u00eancias e Tecnologia FCT, Universidade Nova de Lisboa, Campus da Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9467-3825","authenticated-orcid":false,"given":"S\u00f3nia","family":"Cotrim Marques","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Instituto Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"},{"name":"Instituto Portugu\u00eas do Mar e da Atmosfera (IPMA) Rua Alfredo Magalh\u00e3es Ramalho, 6, 1449-006 Lisboa, Portugal"}]},{"given":"Paulo","family":"Marques Nunes","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Instituto Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"given":"Rui","family":"Pedrosa","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Instituto Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"given":"S\u00e9rgio Miguel","family":"Leandro","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Instituto Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,22]]},"reference":[{"key":"ref_1","unstructured":"Moreira, A.C., Caetano, P., Correia, S., Brasier, C.M., and Ferraz, J.F.P. 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