{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:05Z","timestamp":1770336005253,"version":"3.49.0"},"reference-count":48,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interreg Program for the financial support of the Project IBERPHENOL","award":["0377_IBERPHENOL_6_E"],"award-info":[{"award-number":["0377_IBERPHENOL_6_E"]}]},{"name":"Funda\u00e7\u00e3o para a ci\u00eancia e Tecnologia","award":["UID\/AGR\/04033\/2019, UID\/EQU\/00511\/2019"],"award-info":[{"award-number":["UID\/AGR\/04033\/2019, UID\/EQU\/00511\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Some diet profiles are associated with the risk of developing cancer; however, some nutrients show protective effects. Porphyra umbilicalis is widely consumed, having a balanced nutritional profile; however, its potential for cancer chemoprevention still needs comprehensive studies. In this study, we incorporated P. umbilicalis into the diet of mice transgenic for the human papillomavirus type 16 (HPV16), which spontaneously develop pre-malignant and malignant lesions, and determined whether this seaweed was able to block lesion development. Forty-four 20-week-old HPV+\/\u2212 and HPV\u2212\/\u2212 mice were fed either a base diet or a diet supplemented with 10% seaweed. At the end of the study, skin samples were examined to classify HPV16-induced lesions. The liver was also screened for potential toxic effects of the seaweed. Blood was used to study toxicological parameters and to perform comet and micronucleus genotoxicity tests. P. umbilicalis significantly reduced the incidence of pre-malignant dysplastic lesions, completely abrogating them in the chest skin. These results suggest that P. umbilicalis dietary supplementation has the potential to block the development of pre-malignant skin lesions and indicate its antigenotoxic activity against HPV-induced DNA damage. Further studies are needed to establish the seaweed as a functional food and clarify the mechanisms whereby this seaweed blocks multistep carcinogenesis induced by HPV.<\/jats:p>","DOI":"10.3390\/md17110615","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T05:18:26Z","timestamp":1572499106000},"page":"615","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Dietary Supplementation with the Red Seaweed Porphyra umbilicalis Protects against DNA Damage and Pre-Malignant Dysplastic Skin Lesions in HPV-Transgenic Mice"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0426-6335","authenticated-orcid":false,"given":"Susana","family":"Santos","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6652-9770","authenticated-orcid":false,"given":"Tiago","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"}]},{"given":"Jos\u00e9","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"}]},{"given":"Maria J.","family":"Pires","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"}]},{"given":"Aura","family":"Cola\u00e7o","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"},{"name":"Animal and Veterinary Research Center (CECAV), 5001-801 Vila Real, Portugal"}]},{"given":"S\u00edlvia","family":"Lemos","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2151-2449","authenticated-orcid":false,"given":"Rui M.","family":"Gil da Costa","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"},{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"Molecular Oncology and Viral Pathology Group, IPO-Porto Research Center (CI-IPOP), Portuguese Institute of Oncology of Porto (IPO-Porto), 4200-072 Porto, Portugal"}]},{"given":"Rui","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Viral Pathology Group, IPO-Porto Research Center (CI-IPOP), Portuguese Institute of Oncology of Porto (IPO-Porto), 4200-072 Porto, Portugal"},{"name":"Faculty of Medicine, University of Porto (FMUP), 4200-450 Porto, Portugal"},{"name":"CEBIMED, Faculty of Health Sciences, Fernando Pessoa University, 4200-150 Porto, Portugal"},{"name":"LPCC Research Department, Portuguese League against Cancer (NRNorte), 4200-172 Porto, Portugal"}]},{"given":"Margarida M. S. M.","family":"Bastos","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Maria J.","family":"Neuparth","sequence":"additional","affiliation":[{"name":"Research Center in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sports, University of Porto, 4200-450 Porto, Portugal"}]},{"given":"Helena","family":"Abreu","sequence":"additional","affiliation":[{"name":"ALGAplus, Lda., PCI-Creative Science Park, 3830-352 \u00cdlhavo, Portugal"}]},{"given":"Rui","family":"Pereira","sequence":"additional","affiliation":[{"name":"ALGAplus, Lda., PCI-Creative Science Park, 3830-352 \u00cdlhavo, Portugal"}]},{"given":"M\u00e1rio","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4461-7021","authenticated-orcid":false,"given":"Isabel","family":"Gaiv\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Genetic and Biotechnology, CECAV, UTAD, 5001-801 Vila Real, Portugal"}]},{"given":"Eduardo","family":"Rosa","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"},{"name":"Department of Agronomy, UTAD, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9519-4044","authenticated-orcid":false,"given":"Paula A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), 5001-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.4161\/psb.6.9.16779","article-title":"Algae as promising organisms for environment and health","volume":"6","author":"Shalaby","year":"2011","journal-title":"Plant Signal. 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