{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T18:27:50Z","timestamp":1778178470810,"version":"3.51.4"},"reference-count":80,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T00:00:00Z","timestamp":1625184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\/MCTES (PIDDAC)","award":["Base Funding\u2014UIDB\/00511\/2020 of the Laboratory for Process Engineering, Environment, Bio-technology and Energy (LEPABE)"],"award-info":[{"award-number":["Base Funding\u2014UIDB\/00511\/2020 of the Laboratory for Process Engineering, Environment, Bio-technology and Energy (LEPABE)"]}]},{"name":"INTERREG V Espanha Portugal (POCTEP)","award":["0302_CVMAR_I_1_P"],"award-info":[{"award-number":["0302_CVMAR_I_1_P"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["952471"],"award-info":[{"award-number":["952471"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["Scientific Employment Stimulus\u2014Individual Call\u2014[CEECIND\/01700\/2017]"],"award-info":[{"award-number":["Scientific Employment Stimulus\u2014Individual Call\u2014[CEECIND\/01700\/2017]"]}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["Grupos de Potencial Crecimiento, IN607B 2018\/2019"],"award-info":[{"award-number":["Grupos de Potencial Crecimiento, IN607B 2018\/2019"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The growing requirement for sustainable processes has boosted the development of biodegradable plastic-based materials incorporating bioactive compounds obtained from waste, adding value to these products. Chitosan (Ch) is a biopolymer that can be obtained by deacetylation of chitin (found abundantly in waste from the fishery industry) and has valuable properties such as biocompatibility, biodegradability, antimicrobial activity, and easy film-forming ability. This study aimed to produce and characterize poly(lactic acid) (PLA) surfaces coated with \u03b2-chitosan and \u03b2-chitooligosaccharides from a Loligo opalescens pen with different molecular weights for application in the food industry. The PLA films with native and depolymerized Ch were functionalized through plasma oxygen treatment followed by dip-coating, and their physicochemical properties were assessed by Fourier-transform infrared spectroscopy, X-ray diffraction, water contact angle, and scanning electron microscopy. Their antimicrobial properties were assessed against Escherichia coli and Pseudomonas putida, where Ch-based surfaces reduced the number of biofilm viable, viable but nonculturable, and culturable cells by up to 73%, 74%, and 87%, respectively, compared to PLA. Biofilm growth inhibition was confirmed by confocal laser scanning microscopy. Results suggest that Ch films of higher molecular weight had higher antibiofilm activity under the food storage conditions mimicked in this work, contributing simultaneously to the reuse of marine waste.<\/jats:p>","DOI":"10.3390\/md19070384","type":"journal-article","created":{"date-parts":[[2021,7,4]],"date-time":"2021-07-04T22:34:36Z","timestamp":1625438076000},"page":"384","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Effect of Molecular Weight on the Antimicrobial Activity of Chitosan from Loligo opalescens for Food Packaging Applications"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8992-1097","authenticated-orcid":false,"given":"Luciana C.","family":"Gomes","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Sara I.","family":"Faria","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7336-4049","authenticated-orcid":false,"given":"Jesus","family":"Valcarcel","sequence":"additional","affiliation":[{"name":"Grupo de Reciclado y Valorizaci\u00f3n de Materiales Residuales (REVAL), Instituto de Investigaciones Marinas (IIM-CSIC), C\/Eduardo Cabello, 6, CP36208 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1122-4726","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Grupo de Reciclado y Valorizaci\u00f3n de Materiales Residuales (REVAL), Instituto de Investigaciones Marinas (IIM-CSIC), C\/Eduardo Cabello, 6, CP36208 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6614-3942","authenticated-orcid":false,"given":"Miguel A.","family":"Cerqueira","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory, Department of Life Sciences, Av. Mestre Jos\u00e9 Veiga s\/n, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6637-3462","authenticated-orcid":false,"given":"Lorenzo","family":"Pastrana","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory, Department of Life Sciences, Av. Mestre Jos\u00e9 Veiga s\/n, 4715-330 Braga, Portugal"}]},{"given":"Ana I.","family":"Bourbon","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory, Department of Life Sciences, Av. Mestre Jos\u00e9 Veiga s\/n, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-1037","authenticated-orcid":false,"given":"Filipe J.","family":"Mergulh\u00e3o","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"D\u00edez-Pascual, A.M. (2020). Antimicrobial Polymer-Based Materials for Food Packaging Applications. 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