{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T21:13:01Z","timestamp":1776978781636,"version":"3.51.4"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T00:00:00Z","timestamp":1631836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0140\/202019"],"award-info":[{"award-number":["LA\/P\/0140\/202019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04129\/2020"],"award-info":[{"award-number":["UIDP\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04129\/2020"],"award-info":[{"award-number":["UIDB\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/131947\/2017"],"award-info":[{"award-number":["SFRH\/BD\/131947\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Marine environments comprise almost three quarters of Earth\u2019s surface, representing the largest ecosystem of our planet. The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS). Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction. All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions. Non-carbohydrate substituents, such as acetyl (0.5\u20132.1 wt%), pyruvyl (0.2\u20134.9 wt%), succinyl (1\u20131.8 wt%), and sulfate (1.98\u20133.43 wt%); and few peptides (1.72\u20136.77 wt%) were also detected. Thermal analysis demonstrated that the EPS had a degradation temperature above 260 \u00b0C, and high char yields (32\u201353%). Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions. Thus, these EPS could be versatile materials for different applications.<\/jats:p>","DOI":"10.3390\/md19090522","type":"journal-article","created":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T00:13:47Z","timestamp":1631924027000},"page":"522","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6143-9170","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Conc\u00f3rdio-Reis","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB\u2013Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2013Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4117-5582","authenticated-orcid":false,"given":"V\u00edtor D.","family":"Alves","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food\u2014Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"given":"Xavier","family":"Moppert","sequence":"additional","affiliation":[{"name":"Pacific Biotech SAS, BP 140 289, 98 701 Arue, Tahiti, French Polynesia"}]},{"given":"Jean","family":"Gu\u00e9zennec","sequence":"additional","affiliation":[{"name":"AiMB (Advices in Marine Biotechnology), 17 Rue d\u2019Ouessant, 29280 Plouzan\u00e9, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-4640","authenticated-orcid":false,"given":"Filomena","family":"Freitas","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2013Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2013Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4000-1836","authenticated-orcid":false,"given":"Maria A. M.","family":"Reis","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2013Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2013Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.nbt.2016.02.005","article-title":"Exopolysaccharide Production by a Marine Pseudoalteromonas Sp. Strain Isolated from Madeira Archipelago Ocean Sediments","volume":"33","author":"Roca","year":"2016","journal-title":"New Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Casillo, A., Lanzetta, R., Parrilli, M., and Corsaro, M.M. (2018). Exopolysaccharides from Marine and Marine Extremophilic Bacteria: Structures, Properties, Ecological Roles and Applications. Mar. Drugs, 16.","DOI":"10.3390\/md16020069"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1111\/jam.12184","article-title":"Partial Characterization of an Exopolysaccharide Secreted by a Marine Bacterium, Vibrio Neocaledonicus Sp. Nov., from New Caledonia","volume":"114","author":"Chalkiadakis","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"114999","DOI":"10.1016\/j.carbpol.2019.114999","article-title":"Structure, Rheology, and Copper-Complexation of a Hyaluronan-like Exopolysaccharide from Vibrio","volume":"222","author":"Ferreira","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/sj.jim.7000298","article-title":"Deep-Sea Hydrothermal Vents: A New Source of Innovative Bacterial Exopolysaccharides of Biotechnological Interest?","volume":"29","author":"Guezennec","year":"2002","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Delbarre-Ladrat, C., Salas, M.L., Sinquin, C., Zykwinska, A., and Colliec-Jouault, S. (2017). Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity. Microorganisms, 5.","DOI":"10.3390\/microorganisms5030063"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1007\/s00248-004-0093-8","article-title":"Chemical Characterization of Exopolysaccharides from Antarctic Marine Bacteria","volume":"49","author":"Nichols","year":"2005","journal-title":"Microb. Ecol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/j.carbpol.2011.08.083","article-title":"Microbial Exopolysaccharides: Main Examples of Synthesis, Excretion, Genetics and Extraction","volume":"87","author":"Donot","year":"2012","journal-title":"Carbohydr. Polym."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jenvman.2014.05.010","article-title":"Extracellular Polymeric Substances of Bacteria and Their Potential Environmental Applications","volume":"144","author":"More","year":"2014","journal-title":"J. Environ. Manag."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.carbpol.2018.10.011","article-title":"Extremophilic Exopolysaccharides: A Review and New Perspectives on Engineering Strategies and Applications","volume":"205","author":"Wang","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Cai, R., Zhang, W., Fu, Y., and Jiao, N. (2017). A Novel Exopolysaccharide with Metal Adsorption Capacity Produced by a Marine Bacterium Alteromonas sp. JL2810. Mar. Drugs, 15.","DOI":"10.3390\/md15060175"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1016\/j.biortech.2017.05.092","article-title":"Engineering Aspects of Microbial Exopolysaccharide Production","volume":"245","author":"Freitas","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ijbiomac.2019.03.048","article-title":"A Bioactive Exopolysaccharide from Marine Bacteria Alteromonas sp. PRIM-28 and Its Role in Cell Proliferation and Wound Healing in Vitro","volume":"131","author":"Sahana","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zarandona, I., Estupi\u00f1\u00e1n, M., P\u00e9rez, C., Alonso-S\u00e1ez, L., Guerrero, P., and de la Caba, K. (2020). Chitosan Films Incorporated with Exopolysaccharides from Deep Seawater Alteromonas sp.. Mar. Drugs, 18.","DOI":"10.3390\/md18090447"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s10126-004-5118-2","article-title":"Bacterial Exopolysaccharides from Extreme Marine Environments with Special Consideration of the Southern Ocean, Sea Ice, and Deep-Sea Hydrothermal Vents: A Review","volume":"7","author":"Nichols","year":"2005","journal-title":"Mar. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.procbio.2010.09.001","article-title":"Microbial Mats in French Polynesia and Their Biotechnological Applications","volume":"46","author":"Moppert","year":"2011","journal-title":"Process. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.carbpol.2018.01.086","article-title":"Enzymatic Depolymerization of the GY785 Exopolysaccharide Produced by the Deep-Sea Hydrothermal Bacterium Alteromonas infernus: Structural Study and Enzyme Activity Assessment","volume":"188","author":"Zykwinska","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0144-8617(97)00109-4","article-title":"Chelating Properties of Bacterial Exopolysaccharides from Deep-Sea Hydrothermal Vents","volume":"35","author":"Olier","year":"1998","journal-title":"Carbohydr. Polym."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/j.1365-2672.2008.03789.x","article-title":"Characterization of Exopolysaccharides Produced by Three Moderately Halophilic Bacteria Belonging to the Family Alteromonadaceae","volume":"105","author":"Mata","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-37720-2","article-title":"Genomic Analyses of Two Alteromonas stellipolaris Strains Reveal Traits with Potential Biotechnological Applications","volume":"9","author":"Torres","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.carbpol.2012.04.059","article-title":"Structural Data on a Bacterial Exopolysaccharide Produced by a Deep-Sea Alteromonas macleodii Strain","volume":"90","author":"Ropartz","year":"2012","journal-title":"Carbohydr. Polym."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3389\/fmicb.2015.00288","article-title":"Exopolysaccharides Enriched in Rare Sugars: Bacterial Sources, Production, and Applications","volume":"6","author":"Roca","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.3390\/md13052967","article-title":"Marine Polysaccharides from Algae with Potential Biomedical Applications","volume":"13","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Liu, L., Pohnert, G., and Wei, D. (2016). Extracellular Metabolites from Industrial Microalgae and Their Biotechnological Potential. Mar. Drugs, 14.","DOI":"10.3390\/md14100191"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/j.biotechadv.2016.08.004","article-title":"Overview of Microalgal Extracellular Polymeric Substances (EPS) and Their Applications","volume":"34","author":"Xiao","year":"2016","journal-title":"Biotechnol. Adv."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Singh, S., Kant, C., Yadav, R.K., Reddy, Y.P., and Abraham, G. (2019). Cyanobacterial Exopolysaccharides: Composition, Biosynthesis, and Biotechnological Applications. Cyanobacteria, Elsevier.","DOI":"10.1016\/B978-0-12-814667-5.00017-9"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1007\/s00284-002-3922-3","article-title":"A Novel, Highly Viscous Polysaccharide Excreted by an Alteromonas Isolated from a Deep-Sea Hydrothermal Vent Shrimp","volume":"46","author":"Lohier","year":"2003","journal-title":"Curr. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.ijbiomac.2020.07.072","article-title":"Silver Nanocomposites Based on the Bacterial Fucose-Rich Polysaccharide Secreted by Enterobacter A47 for Wound Dressing Applications: Synthesis, Characterization and in Vitro Bioactivity","volume":"163","author":"Pereira","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Chen, X., Song, L., Wang, H., Liu, S., Yu, H., Wang, X., Li, R., Liu, T., and Li, P. (2019). Partial Characterization, the Immune Modulation and Anticancer Activities of Sulfated Polysaccharides from Filamentous Microalgae Tribonema sp.. Molecules, 24.","DOI":"10.3390\/molecules24020322"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9243","DOI":"10.1039\/C7RA11153C","article-title":"Preliminary Characterization of the Structure and Immunostimulatory and Anti-Aging Properties of the Polysaccharide Fraction of Haematococcus pluvialis","volume":"8","author":"Liu","year":"2018","journal-title":"RSC Adv."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12906-016-1198-6","article-title":"Partial Characterization and Antioxidant and Antiproliferative Activities of the Aqueous Extracellular Polysaccharides from the Thermophilic Microalgae Graesiella sp.","volume":"16","author":"Trabelsi","year":"2016","journal-title":"BMC Complement. Altern. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1016\/j.ijbiomac.2018.09.147","article-title":"Purification and Structural-Functional Characterization of an Exopolysaccharide from Bacillus licheniformis PASS26 with in-Vitro Antitumor and Wound Healing Activities","volume":"120","author":"Insulkar","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.carbpol.2017.11.086","article-title":"Rheological Characterization of the Exopolysaccharide Paenan in Surfactant Systems","volume":"181","author":"Schmid","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1016\/j.ijbiomac.2020.08.114","article-title":"Rheological Behaviors of a Novel Exopolysaccharide Produced by Sphingomonas WG and the Potential Application in Enhanced Oil Recovery","volume":"162","author":"Ji","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.ijbiomac.2011.02.012","article-title":"Influence of Temperature on the Rheological Behavior of a New Fucose-Containing Bacterial Exopolysaccharide","volume":"48","author":"Cruz","year":"2011","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.carbpol.2009.10.026","article-title":"Effect of Temperature on the Dynamic and Steady-Shear Rheology of a New Microbial Extracellular Polysaccharide Produced from Glycerol Byproduct","volume":"79","author":"Alves","year":"2010","journal-title":"Carbohydr. Polym."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.carbpol.2010.07.034","article-title":"Fucose-Containing Exopolysaccharide Produced by the Newly Isolated Enterobacter Strain A47 DSM 23139","volume":"83","author":"Freitas","year":"2011","journal-title":"Carbohydr. Polym."},{"key":"ref_38","first-page":"99","article-title":"Rheological Equations from Molecular Network Theories","volume":"16","author":"Carreau","year":"1972","journal-title":"J. Rheol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.ijbiomac.2015.05.029","article-title":"Rheological Studies of the Fucose-Rich Exopolysaccharide FucoPol","volume":"79","author":"Torres","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1039\/D0BM00055H","article-title":"Rational Design and Latest Advances of Polysaccharide-Based Hydrogels for Wound Healing","volume":"8","author":"Hu","year":"2020","journal-title":"Biomater. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.carbpol.2016.01.056","article-title":"Assembly of HE800 Exopolysaccharide Produced by a Deep-Sea Hydrothermal Bacterium into Microgels for Protein Delivery Applications","volume":"142","author":"Zykwinska","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1016\/j.biomaterials.2011.01.004","article-title":"A Review of the Biological Response to Ionic Dissolution Products from Bioactive Glasses and Glass-Ceramics","volume":"32","author":"Hoppe","year":"2011","journal-title":"Biomaterials"},{"key":"ref_43","first-page":"42","article-title":"Studies on Marine Bacteria. I. The Cultural Requirements of Heterotrophic Aerobes","volume":"4","author":"Zobell","year":"1941","journal-title":"J. Mar. Res."},{"key":"ref_44","first-page":"10","article-title":"The Growth and Viability of Sixty-Three Species of Marine Bacteria as Influenced by Hydrostatic Pressure","volume":"11","author":"Oppenheimer","year":"1952","journal-title":"J. Mar. Res."},{"key":"ref_45","first-page":"67","article-title":"Description of a New Polymer-Secreting Bacterium from a Deep-Sea Hydrothermal Vent, Alteromonas macleodii Subsp","volume":"62","author":"Raguenes","year":"1996","journal-title":"fijiensis, and Preliminary Characterization of the Polymer. Appl. Environ. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1046\/j.1365-2672.2002.01689.x","article-title":"A Novel Polymer Produced by a Bacterium Isolated from a Deep-Sea Hydrothermal Vent Polychaete Annelid","volume":"93","author":"Raguenes","year":"2002","journal-title":"J. Appl. Microbiol."},{"key":"ref_47","first-page":"36","article-title":"Uber Den Gehalt Der Wichtigsten Protein Der Nahrangsmittel an Kohlehydrat Andu\u00a8ber Ein Kolorimetriches Verfharen Zur Quantitativen Beshimmung von Stucksoffreiem Zucker in Elweiss","volume":"215","author":"Tilmans","year":"1929","journal-title":"Biochim. Ztg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1042\/bj0251062","article-title":"The Carbohydrate Complex of Serum Protein II: Improved Method for Isolation and Determination of Structure. Isolation of Glucosaminodimannose from Protein of Blood","volume":"25","author":"Rimington","year":"1931","journal-title":"Biochem. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/0003-2697(73)90377-1","article-title":"New Method for Quantitative Determination of Uronic Acid","volume":"54","author":"Blumenkrantz","year":"1973","journal-title":"Anal. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1042\/bj1510491","article-title":"Characterization by Gas Chromatography-Mass Spectrometry of Pertrimethylsilyl Glycosides Obtained in the Methanolysis of Glycoproteins and Glycolipids","volume":"151","author":"Kamerling","year":"1975","journal-title":"Biochem. J."},{"key":"ref_51","unstructured":"Montreuil, J., Bouquelet, S., Debray, H., Fournet, B., Spik, G., and Strecker, G. (1986). Glycoproteins. Carbohydrate Analysis: A Practical Approach, IRL Press."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Conc\u00f3rdio-Reis, P., Reis, M.A.M., and Freitas, F. (2020). Biosorption of Heavy Metals by the Bacterial Exopolysaccharide FucoPol. Appl. Sci., 10.","DOI":"10.3390\/app10196708"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0922-338X(97)82538-X","article-title":"Acidic Exopolysaccharide Produced by Enterobacter sp.","volume":"84","author":"Shimada","year":"1997","journal-title":"J. Ferment. Bioeng."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/19\/9\/522\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:01:23Z","timestamp":1760166083000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/19\/9\/522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,17]]},"references-count":53,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,9]]}},"alternative-id":["md19090522"],"URL":"https:\/\/doi.org\/10.3390\/md19090522","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,17]]}}}