{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T14:39:06Z","timestamp":1769265546418,"version":"3.49.0"},"reference-count":60,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["691102"],"award-info":[{"award-number":["691102"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010686","name":"H2020 European Institute of Innovation and Technology","doi-asserted-by":"publisher","award":["654008"],"award-info":[{"award-number":["654008"]}],"id":[{"id":"10.13039\/100010686","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["\u201cNorma Transit\u00f3ria\u201d -DL57\/2016\/CP1361\/CT0020"],"award-info":[{"award-number":["\u201cNorma Transit\u00f3ria\u201d -DL57\/2016\/CP1361\/CT0020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["\u201cNorma Transit\u00f3ria\u201d -DL57\/2016\/CP1361\/CT0011"],"award-info":[{"award-number":["\u201cNorma Transit\u00f3ria\u201d -DL57\/2016\/CP1361\/CT0011"]}]},{"name":"CRESC Algarve 2020 and COMPETE 2020","award":["EMBRC.PT ALG-01-0145-FEDER-022121"],"award-info":[{"award-number":["EMBRC.PT ALG-01-0145-FEDER-022121"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Fish skin has been gaining attention due to its efficacy as a human-wound-treatment product and to identify factors promoting its enhanced action. Skin fibroblasts have a central role in maintaining skin integrity and secrete extra cellular matrix (ECM) proteins, growth factors and cytokines to rapidly repair lesions and prevent further damage or infection. The effects on scratch repair of the ubiquitous but poorly characterized ECM protein, cartilage acidic protein 1 (CRTAC1), from piscine and human sources were compared using a zebrafish SJD.1 primary fibroblast cell line. A classic in vitro cell scratch assay, immunofluorescence, biosensor and gene expression analysis were used. Our results demonstrated that the duplicate sea bass Crtac1a and Crtac1b proteins and human CRTAC-1A all promoted SJD.1 primary fibroblast migration in a classic scratch assay and in an electric cell impedance sensing assay. The immunofluorescence analysis revealed that CRTAC1 enhanced cell migration was most likely caused by actin-driven cytoskeletal changes and the cellular transcriptional response was most affected in the early stage (6 h) of scratch repair. In summary, our results suggest that CRTAC1 may be an important factor in fish skin promoting damage repair.<\/jats:p>","DOI":"10.3390\/md19100541","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T04:55:33Z","timestamp":1632718533000},"page":"541","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4144-3452","authenticated-orcid":false,"given":"Rute Castelo","family":"F\u00e9lix","sequence":"first","affiliation":[{"name":"Centro de Ci\u00eancias do Mar (CCMAR), Comparative Endocrinology and Integrative Biology Group, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9474-522X","authenticated-orcid":false,"given":"Liliana","family":"Anjos","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias do Mar (CCMAR), Comparative Endocrinology and Integrative Biology Group, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Rita Alves","family":"Costa","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias do Mar (CCMAR), Comparative Endocrinology and Integrative Biology Group, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9155-0889","authenticated-orcid":false,"given":"Sophia","family":"Letsiou","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Scientific Affairs, APIVITA SA, Industrial Park of Markopoulo Mesogaias, Markopoulo Attikis, 19003 Athens, Greece"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1366-0246","authenticated-orcid":false,"given":"Deborah Mary","family":"Power","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias do Mar (CCMAR), Comparative Endocrinology and Integrative Biology Group, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jsbmb.2013.02.006","article-title":"Steroidogenesis in the skin: Implications for local immune functions","volume":"137","author":"Slominski","year":"2013","journal-title":"J. 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