{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T09:20:36Z","timestamp":1778232036729,"version":"3.51.4"},"reference-count":169,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (Portugal)","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (Portugal)","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (Portugal)","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (Portugal)","award":["LA\/P\/0101\/2020"],"award-info":[{"award-number":["LA\/P\/0101\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (Portugal)","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (Portugal)","award":["UIDP\/04326\/2020"],"award-info":[{"award-number":["UIDP\/04326\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industrial production. Several strain improvement technologies have been developed towards creating more stress tolerant and productive strains. While classical methods of forward genetics have been extensively used to determine gene function of randomly generated mutants, reverse genetics has been explored to generate specific mutations and target phenotypes. Site-directed mutagenesis can be accomplished by employing different gene editing tools, which enable the generation of tailor-made genotypes. Nevertheless, strategies promoting the selection of randomly generated mutants avoid the introduction of foreign genetic material. In this paper, we review different microalgal strain improvement approaches and their applications, with a primary focus on random mutagenesis. Current challenges hampering strain improvement, selection, and commercialization will be discussed. The combination of these approaches with high-throughput technologies, such as fluorescence-activated cell sorting, as tools to select the most promising mutants, will also be discussed.<\/jats:p>","DOI":"10.3390\/md20070440","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:21:50Z","timestamp":1656634910000},"page":"440","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":135,"title":["Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9465-7681","authenticated-orcid":false,"given":"Mafalda","family":"Trov\u00e3o","sequence":"first","affiliation":[{"name":"Allmicroalgae Natural Products S.A., R&D Department, Rua 25 de Abril s\/n, 2445-413 Pataias, Portugal"},{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0961-5397","authenticated-orcid":false,"given":"Lisa M.","family":"Sch\u00fcler","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Adriana","family":"Machado","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., R&D Department, Rua 25 de Abril s\/n, 2445-413 Pataias, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2838-6262","authenticated-orcid":false,"given":"Gabriel","family":"Bombo","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9380-4945","authenticated-orcid":false,"given":"Sofia","family":"Navalho","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Ana","family":"Barros","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., R&D Department, Rua 25 de Abril s\/n, 2445-413 Pataias, Portugal"}]},{"given":"Hugo","family":"Pereira","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Joana","family":"Silva","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., R&D Department, Rua 25 de Abril s\/n, 2445-413 Pataias, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-4640","authenticated-orcid":false,"given":"Filomena","family":"Freitas","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-693X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Varela","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"CCMAR\u2014Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7341","DOI":"10.1021\/jf401957z","article-title":"Potential and limitation of a new defatted diatom microalgal biomass in replacing soybean meal and corn in diets for broiler chickens","volume":"61","author":"Austic","year":"2013","journal-title":"J. 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