{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T19:13:03Z","timestamp":1770837183523,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T00:00:00Z","timestamp":1664496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["805411"],"award-info":[{"award-number":["805411"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["PD\/BD\/135252\/2017"],"award-info":[{"award-number":["PD\/BD\/135252\/2017"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["IF\/00347\/2015"],"award-info":[{"award-number":["IF\/00347\/2015"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["0474_BLUEBIOLAB_1_E"],"award-info":[{"award-number":["0474_BLUEBIOLAB_1_E"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["EAPA_151\/2016"],"award-info":[{"award-number":["EAPA_151\/2016"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Union\u2019s Horizon 2020 research and innovation programme","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["805411"],"award-info":[{"award-number":["805411"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["PD\/BD\/135252\/2017"],"award-info":[{"award-number":["PD\/BD\/135252\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["IF\/00347\/2015"],"award-info":[{"award-number":["IF\/00347\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["0474_BLUEBIOLAB_1_E"],"award-info":[{"award-number":["0474_BLUEBIOLAB_1_E"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["EAPA_151\/2016"],"award-info":[{"award-number":["EAPA_151\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"INTERREG Espa\u00f1a-Portugal 2014-2020","doi-asserted-by":"publisher","award":["805411"],"award-info":[{"award-number":["805411"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"INTERREG Espa\u00f1a-Portugal 2014-2020","doi-asserted-by":"publisher","award":["PD\/BD\/135252\/2017"],"award-info":[{"award-number":["PD\/BD\/135252\/2017"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"INTERREG Espa\u00f1a-Portugal 2014-2020","doi-asserted-by":"publisher","award":["IF\/00347\/2015"],"award-info":[{"award-number":["IF\/00347\/2015"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"INTERREG Espa\u00f1a-Portugal 2014-2020","doi-asserted-by":"publisher","award":["0474_BLUEBIOLAB_1_E"],"award-info":[{"award-number":["0474_BLUEBIOLAB_1_E"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"INTERREG Espa\u00f1a-Portugal 2014-2020","doi-asserted-by":"publisher","award":["EAPA_151\/2016"],"award-info":[{"award-number":["EAPA_151\/2016"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"INTERREG Espa\u00f1a-Portugal 2014-2020","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Atlantic Area Programme","doi-asserted-by":"publisher","award":["805411"],"award-info":[{"award-number":["805411"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Atlantic Area Programme","doi-asserted-by":"publisher","award":["PD\/BD\/135252\/2017"],"award-info":[{"award-number":["PD\/BD\/135252\/2017"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Atlantic Area Programme","doi-asserted-by":"publisher","award":["IF\/00347\/2015"],"award-info":[{"award-number":["IF\/00347\/2015"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Atlantic Area Programme","doi-asserted-by":"publisher","award":["0474_BLUEBIOLAB_1_E"],"award-info":[{"award-number":["0474_BLUEBIOLAB_1_E"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Atlantic Area Programme","doi-asserted-by":"publisher","award":["EAPA_151\/2016"],"award-info":[{"award-number":["EAPA_151\/2016"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Atlantic Area Programme","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"NORTE2020\/PT2020 Programme","doi-asserted-by":"publisher","award":["805411"],"award-info":[{"award-number":["805411"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"NORTE2020\/PT2020 Programme","doi-asserted-by":"publisher","award":["PD\/BD\/135252\/2017"],"award-info":[{"award-number":["PD\/BD\/135252\/2017"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"NORTE2020\/PT2020 Programme","doi-asserted-by":"publisher","award":["IF\/00347\/2015"],"award-info":[{"award-number":["IF\/00347\/2015"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"NORTE2020\/PT2020 Programme","doi-asserted-by":"publisher","award":["0474_BLUEBIOLAB_1_E"],"award-info":[{"award-number":["0474_BLUEBIOLAB_1_E"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"NORTE2020\/PT2020 Programme","doi-asserted-by":"publisher","award":["EAPA_151\/2016"],"award-info":[{"award-number":["EAPA_151\/2016"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"NORTE2020\/PT2020 Programme","doi-asserted-by":"publisher","award":["Norte-01-0145-FEDER-000040"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000040"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The successful integration of transplanted three-dimensional tissue engineering (TE) constructs depends greatly on their rapid vascularization. Therefore, it is essential to address this vascularization issue in the initial design of constructs for perfused tissues. Two of the most important variables in this regard are scaffold composition and cell sourcing. Collagens with marine origins overcome some issues associated with mammal-derived collagen while maintaining their advantages in terms of biocompatibility. Concurrently, the freshly isolated stromal vascular fraction (SVF) of adipose tissue has been proposed as an advantageous cell fraction for vascularization purposes due to its highly angiogenic properties, allowing extrinsic angiogenic growth factor-free vascularization strategies for TE applications. In this study, we aimed at understanding whether marine collagen 3D matrices could support cryopreserved human SVF in maintaining intrinsic angiogenic properties observed for fresh SVF. For this, cryopreserved human SVF was seeded on blue shark collagen sponges and cultured up to 7 days in a basal medium. The secretome profile of several angiogenesis-related factors was studied throughout culture times and correlated with the expression pattern of CD31 and CD146, which showed the formation of a prevascular network. Upon in ovo implantation, increased vessel recruitment was observed in prevascularized sponges when compared with sponges without SVF cells. Immunohistochemistry for CD31 demonstrated the improved integration of prevascularized sponges within chick chorioalantoic membrane (CAM) tissues, while in situ hybridization showed human cells lining blood vessels. These results demonstrate the potential of using cryopreserved SVF combined with marine collagen as a streamlined approach to improve the vascularization of TE constructs.<\/jats:p>","DOI":"10.3390\/md20100623","type":"journal-article","created":{"date-parts":[[2022,10,9]],"date-time":"2022-10-09T01:43:11Z","timestamp":1665279791000},"page":"623","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3103-6703","authenticated-orcid":false,"given":"Sara","family":"Freitas-Ribeiro","sequence":"first","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, Braga, 4710-057 Guimar\u00e3es, Portugal"}]},{"given":"Gabriela S.","family":"Diogo","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, Braga, 4710-057 Guimar\u00e3es, Portugal"}]},{"given":"Catarina","family":"Oliveira","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, Braga, 4710-057 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3868-0251","authenticated-orcid":false,"given":"Albino","family":"Martins","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, Braga, 4710-057 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8520-603X","authenticated-orcid":false,"given":"Tiago H.","family":"Silva","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, Braga, 4710-057 Guimar\u00e3es, Portugal"}]},{"given":"Mariana","family":"Jarnalo","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, and Burn Unity, Centro Hospitalar de S\u00e3o Jo\u00e3o, 4200-319 Porto, Portugal"},{"name":"Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4658-1889","authenticated-orcid":false,"given":"Ricardo","family":"Horta","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, and Burn Unity, Centro Hospitalar de S\u00e3o Jo\u00e3o, 4200-319 Porto, Portugal"},{"name":"Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, Braga, 4710-057 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5521-950X","authenticated-orcid":false,"given":"Rog\u00e9rio P.","family":"Pirraco","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group, I3Bs\u2014Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, Braga, 4710-057 Guimar\u00e3es, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.5661\/bger-26-163","article-title":"Supply of Nutrients to Cells in Engineered Tissues","volume":"26","author":"Rouwkema","year":"2009","journal-title":"Biotechnol. Genet. Eng. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1002\/aja.1000640203","article-title":"Microscopic observations on the growth of blood capillaries in the living mammal","volume":"64","author":"Clark","year":"1939","journal-title":"Am. J. Anat."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1056\/NEJMoa040455","article-title":"Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Autologous Oral Mucosal Epithelium","volume":"351","author":"Nishida","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1097\/01.tp.0000181163.69108.dd","article-title":"Tissue Cardiomyoplasty Using Bioengineered Contractile Cardiomyocyte Sheets to Repair Damaged Myocardium: Their Integration with Recipient Myocardium","volume":"80","author":"Miyagawa","year":"2005","journal-title":"Transplantation"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.3389\/fbioe.2021.745314","article-title":"Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering","volume":"9","author":"Shafiee","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1080\/15476278.2019.1697597","article-title":"Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose\u2013Collagen Gels","volume":"16","author":"Kniebs","year":"2020","journal-title":"Organogenesis"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.7150\/thno.29552","article-title":"Engineering stem cell cardiac patch with microvascular features representative of native myocardium","volume":"9","author":"Qian","year":"2019","journal-title":"Theranostics"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/S1357-2725(97)00061-7","article-title":"Human microvascular endothelial cells differ from macrovascular endothelial cells in their expression of matrix metalloproteinases","volume":"29","author":"Jackson","year":"1997","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1159\/000331410","article-title":"Molecular Mechanisms Controlling Vascular Lumen Formation in Three-Dimensional Extracellular Matrices","volume":"195","author":"Sacharidou","year":"2012","journal-title":"Cells Tissues Organs"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1161\/ATVBAHA.119.312425","article-title":"Significance of Cellular Cross-Talk in Stromal Vascular Fraction of Adipose Tissue in Neovascularization","volume":"39","author":"Sun","year":"2019","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.jcyt.2013.02.006","article-title":"Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal\/stem cells: A joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT)","volume":"15","author":"Bourin","year":"2013","journal-title":"Cytotherapy"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1089\/ten.teb.2017.0061","article-title":"The Adipose Stromal Vascular Fraction as a Complex Cellular Source for Tissue Engineering Applications","volume":"24","author":"Ramakrishnan","year":"2018","journal-title":"Tissue Eng. Part B Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.actbio.2017.03.034","article-title":"Cell sheet engineering using the stromal vascular fraction of adipose tissue as a vascularization strategy","volume":"55","author":"Costa","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mytsyk, M., Cerino, G., Reid, G., Sole, L., Eckstein, F., Santer, D., and Marsano, A. (2021). Long-Term Severe In Vitro Hypoxia Exposure Enhances the Vascularization Potential of Human Adipose Tissue-Derived Stromal Vascular Fraction Cell Engineered Tissues. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22157920"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Coppola, D., Oliviero, M., Vitale, G.A., Lauritano, C., D\u2019Ambra, I., Iannace, S., and De Pascale, D. (2020). Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications. Mar. Drugs, 18.","DOI":"10.3390\/md18040214"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1002\/bies.20636","article-title":"The collagen family members as cell adhesion proteins","volume":"29","author":"Heino","year":"2007","journal-title":"BioEssays"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"221ra14","DOI":"10.1126\/scitranslmed.3006894","article-title":"Bioengineering Dermo-Epidermal Skin Grafts with Blood and Lymphatic Capillaries","volume":"6","author":"Marino","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/term.3003","article-title":"Engineered skin graft with stromal vascular fraction cells encapsulated in fibrin\u2013collagen hydrogel: A clinical study for diabetic wound healing","volume":"14","author":"Nilforoushzadeh","year":"2020","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1039\/D0LC00099J","article-title":"Matrix density drives 3D organotypic lymphatic vessel activation in a microfluidic model of the breast tumor microenvironment","volume":"20","author":"Ayuso","year":"2020","journal-title":"Lab Chip"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.actbio.2016.08.028","article-title":"Collagen I hydrogel microstructure and composition conjointly regulate vascular network formation","volume":"44","author":"McCoy","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1089\/ten.tea.2008.0550","article-title":"Formation of Human Capillaries In Vitro: The Engineering of Prevascularized Matrices","volume":"16","author":"Schiestl","year":"2010","journal-title":"Tissue Eng. Part A"},{"key":"ref_22","unstructured":"European Medicines Agency (2011). Note for guidance on minimising the risk of transmitting animal spongiform encephalopathy agents via human and veterinary medicinal products (EMA\/410\/01 rev.3). Off. J. Eur. Union, 73, 1\u201318."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1080\/10498850.2013.865283","article-title":"Characterization of Collagen from Different Discarded Fish Species of the West Coast of the Iberian Peninsula","volume":"25","author":"Sotelo","year":"2015","journal-title":"J. Aquat. Food Prod. Technol."},{"key":"ref_24","first-page":"1650","article-title":"Commercial by-catch rates of blue shark (prionace glauca) from longline fisheries in the Canadian Atlantic","volume":"64","author":"Fowler","year":"2009","journal-title":"Collect. Vol. Sci. Pap. ICCAT"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Elango, J., Lee, J.W., Wang, S., Henrotin, Y., De Val, J.E.M.S., Regenstein, J.M., Lim, S.Y., Bao, B., and Wu, W. (2018). Evaluation of Differentiated Bone Cells Proliferation by Blue Shark Skin Collagen via Biochemical for Bone Tissue Engineering. Mar. Drugs, 16.","DOI":"10.3390\/md16100350"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1271\/bbb.61.1919","article-title":"Structural Property and in Vitro Self-assembly of Shark Type I Collagen","volume":"61","author":"Nomura","year":"1997","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"111587","DOI":"10.1016\/j.msec.2020.111587","article-title":"Prionace glauca skin collagen bioengineered constructs as a promising approach to trigger cartilage regeneration","volume":"120","author":"Diogo","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jss.2013.01.005","article-title":"Macroporosity enhances vascularization of electrospun scaffolds","volume":"183","author":"Joshi","year":"2013","journal-title":"J. Surg. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/jbm.a.10542","article-title":"Enhancing the vascularization of three-dimensional porous alginate scaffolds by incorporating controlled release basic fibroblast growth factor microspheres","volume":"65A","author":"Perets","year":"2003","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3294","DOI":"10.1016\/j.actbio.2012.05.019","article-title":"Functional porous hydrogels to study angiogenesis under the effect of controlled release of vascular endothelial growth factor","volume":"8","author":"Oliviero","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.actbio.2019.03.016","article-title":"Upgrading prevascularization in tissue engineering: A review of strategies for promoting highly organized microvascular network formation","volume":"95","author":"Sharma","year":"2019","journal-title":"Acta Biomater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s10616-013-9560-8","article-title":"Effects of long-term serial cell passaging on cell spreading, migration, and cell-surface ultrastructures of cultured vascular endothelial cells","volume":"66","author":"Liao","year":"2014","journal-title":"Cytotechnology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1089\/ten.tea.2014.0299","article-title":"Engineering Vascularized Adipose Tissue Using the Stromal-Vascular Fraction and Fibrin Hydrogels","volume":"21","author":"Wittmann","year":"2015","journal-title":"Tissue Eng. Part A"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1055\/s-0037-1615841","article-title":"The Plasminogen (Fibrinolytic) System","volume":"82","author":"Collen","year":"1999","journal-title":"Thromb. Haemost."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1002\/jcp.1041530117","article-title":"Plasminogen activator inhibitor-1 is induced in migrating endothelial cells","volume":"153","author":"Pepper","year":"1992","journal-title":"J. Cell. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0922-3371(90)90046-Y","article-title":"Proteolytic balance and capillary morphogenesis","volume":"32","author":"Pepper","year":"1990","journal-title":"Cell Differ. Dev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1242\/jcs.112.10.1599","article-title":"The role of matrix metalloproteinase activity in the maturation of human capillary endothelial cells in vitro","volume":"112","author":"Kraling","year":"1999","journal-title":"J. Cell Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1161\/01.RES.0000146672.10582.17","article-title":"Chemokines in the Pathogenesis of Vascular Disease","volume":"95","author":"Charo","year":"2004","journal-title":"Circ. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1182\/blood.V65.1.79.79","article-title":"Monocytes and macrophages synthesize and secrete thrombospondin","volume":"65","author":"Jaffe","year":"1985","journal-title":"Blood"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.1126\/science.1281554","article-title":"Interleukin-8 as a macrophage-derived mediator of angiogenesis","volume":"258","author":"Koch","year":"1992","journal-title":"Science"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3369","DOI":"10.4049\/jimmunol.170.6.3369","article-title":"IL-8 Directly Enhanced Endothelial Cell Survival, Proliferation, and Matrix Metalloproteinases Production and Regulated Angiogenesis","volume":"170","author":"Li","year":"2003","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1182\/blood-2004-08-3178","article-title":"Monocyte chemoattractant protein-1\u2013induced angiogenesis is mediated by vascular endothelial growth factor-A","volume":"105","author":"Hong","year":"2005","journal-title":"Blood"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1161\/01.ATV.0000096208.80992.63","article-title":"Molecular Mechanism and Role of Endothelial Monocyte Chemoattractant Protein-1 Induction by Vascular Endothelial Growth Factor","volume":"23","author":"Yamada","year":"2003","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1182\/blood-2006-07-036400","article-title":"MCP-1 mediates TGF-\u03b2\u2013induced angiogenesis by stimulating vascular smooth muscle cell migration","volume":"109","author":"Ma","year":"2006","journal-title":"Blood"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Mendes, L.F.F., Pirraco, R.P., Szymczyk, W., Frias, A.M., Santos, T.C., Reis, R.L., and Marques, A.P. (2012). Perivascular-Like Cells Contribute to the Stability of the Vascular Network of Osteogenic Tissue Formed from Cell Sheet-Based Constructs. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0041051"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.cardiores.2005.08.003","article-title":"Pericytes influence endothelial cell growth characteristics: Role of plasminogen activator inhibitor type 1 (PAI-1)","volume":"69","author":"McIlroy","year":"2006","journal-title":"Cardiovasc. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"11205","DOI":"10.1073\/pnas.172161899","article-title":"Angiopoietin-2 displays VEGF-dependent modulation of capillary structure and endothelial cell survival in vivo","volume":"99","author":"Lobov","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1083\/jcb.97.5.1648","article-title":"In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices","volume":"97","author":"Montesano","year":"1983","journal-title":"J. Cell Biol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Chen, J., Gao, K., Liu, S., Wang, S., Elango, J., Bao, B., Dong, J., Liu, N., and Wu, W. (2019). Fish Collagen Surgical Compress Repairing Characteristics on Wound Healing Process In Vivo. Mar. Drugs, 17.","DOI":"10.3390\/md17010033"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.msec.2017.06.012","article-title":"Comparison of thermal properties of fish collagen and bovine collagen in the temperature range 298\u2013670 K","volume":"80","author":"Kubisz","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1089\/ten.tea.2010.0148","article-title":"The Correlation Between the Internal Structure and Vascularization of Controllable Porous Bioceramic Materials In Vivo: A Quantitative Study","volume":"16","author":"Bai","year":"2010","journal-title":"Tissue Eng. Part A"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1089\/ten.tec.2014.0454","article-title":"Pore Interconnectivity Influences Growth Factor-Mediated Vascularization in Sphere-Templated Hydrogels","volume":"21","author":"Somo","year":"2015","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1016\/j.biomaterials.2012.12.047","article-title":"Three-dimensional modeling of angiogenesis in porous biomaterial scaffolds","volume":"34","author":"Mehdizadeh","year":"2013","journal-title":"Biomaterials"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.3389\/fmars.2021.713980","article-title":"A Review of Sustainability Concepts in Marine Spatial Planning and the Potential to Supporting the UN Sustainable Development Goal 14","volume":"8","author":"Kirkfeldt","year":"2021","journal-title":"Front. Mar. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1089\/ten.tec.2017.0186","article-title":"The Chorioallantoic Membrane Assay for Biomaterial Testing in Tissue Engineering: A Short-Term In Vivo Preclinical Model","volume":"23","author":"Kanczler","year":"2017","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"119664","DOI":"10.1016\/j.biomaterials.2019.119664","article-title":"Multi-layer pre-vascularized magnetic cell sheets for bone regeneration","volume":"231","author":"Silva","year":"2020","journal-title":"Biomaterials"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"121222","DOI":"10.1016\/j.biomaterials.2021.121222","article-title":"Engineering injectable vascularized tissues from the bottom-up: Dynamics of in-gel extra-spheroid dermal tissue assembly","volume":"279","author":"Neves","year":"2021","journal-title":"Biomaterials"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.lfs.2006.10.020","article-title":"Transplantation of adipose stromal cells, but not mature adipocytes, augments ischemia-induced angiogenesis","volume":"80","author":"Sumi","year":"2007","journal-title":"Life Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1161\/ATVBAHA.110.218206","article-title":"Stromal Vascular Fraction From Adipose Tissue Forms Profound Vascular Network Through the Dynamic Reassembly of Blood Endothelial Cells","volume":"31","author":"Koh","year":"2011","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2542","DOI":"10.1161\/01.ATV.0000190701.92007.6d","article-title":"Novel Autologous Cell Therapy in Ischemic Limb Disease Through Growth Factor Secretion by Cultured Adipose Tissue\u2013Derived Stromal Cells","volume":"25","author":"Nakagami","year":"2005","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00383-015-3808-7","article-title":"Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute","volume":"32","author":"Klar","year":"2016","journal-title":"Pediatr. Surg. Int."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"14252","DOI":"10.1038\/s41598-017-13882-3","article-title":"Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells","volume":"7","author":"Cerino","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"115034","DOI":"10.1016\/j.carbpol.2019.115034","article-title":"Fucoidan from Fucus vesiculosus inhibits new blood vessel formation and breast tumor growth in vivo","volume":"223","author":"Oliveira","year":"2019","journal-title":"Carbohydr. Polym."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/20\/10\/623\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:44:30Z","timestamp":1760143470000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/20\/10\/623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,30]]},"references-count":63,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["md20100623"],"URL":"https:\/\/doi.org\/10.3390\/md20100623","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,30]]}}}