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This study inspected two culture collections comprising 919 host-associated marine bacteria belonging to 55 genera and several thus-far unclassified lineages to identify isolates with potentially rich secondary metabolism and antimicrobial activities. Seventy representative isolates had their genomes mined for secondary metabolite biosynthetic gene clusters (SM-BGCs) and were screened for antimicrobial activities against four pathogenic bacteria and five pathogenic Candida strains. In total, 466 SM-BGCs were identified, with antimicrobial peptide- and polyketide synthase-related SM-BGCs being frequently detected. Only 38 SM-BGCs had similarities greater than 70% to SM-BGCs encoding known compounds, highlighting the potential biosynthetic novelty encoded by these genomes. Cross-streak assays showed that 33 of the 70 genome-sequenced isolates were active against at least one Candida species, while 44 isolates showed activity against at least one bacterial pathogen. Taxon-specific differences in antimicrobial activity among isolates suggested distinct molecules involved in antagonism against bacterial versus Candida pathogens. The here reported culture collections and genome-sequenced isolates constitute a valuable resource of understudied marine bacteria displaying antimicrobial activities and potential for the biosynthesis of novel secondary metabolites, holding promise for a future sustainable production of marine drug leads.<\/jats:p>","DOI":"10.3390\/md21010034","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T03:00:59Z","timestamp":1672628459000},"page":"34","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Marine Sponge and Octocoral-Associated Bacteria Show Versatile Secondary Metabolite Biosynthesis Potential and Antimicrobial Activities against Human Pathogens"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8305-5095","authenticated-orcid":false,"given":"Jo\u00e3o F.","family":"Almeida","sequence":"first","affiliation":[{"name":"iBB\u2014Institute for Bioengineering and Biosciences and i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Av. 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R.","family":"Cleary","sequence":"additional","affiliation":[{"name":"Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6495-2620","authenticated-orcid":false,"given":"Yusheng M.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Marine Recreation, National Penghu University of Science and Technology, Magong City 880-011, Taiwan"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8066-5787","authenticated-orcid":false,"given":"Ars\u00e9nio M.","family":"Fialho","sequence":"additional","affiliation":[{"name":"iBB\u2014Institute for Bioengineering and Biosciences and i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Av. 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Antimicrobial Resistance: Tackling a Crisis for the Health and Wealth of Nations, Amr-Review."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3255","DOI":"10.2147\/IDR.S272733","article-title":"Methicillin-Resistant Staphylococcus aureus (MRSA): One Health Perspective Approach to the Bacterium Epidemiology, Virulence Factors, Antibiotic-Resistance, and Zoonotic Impact","volume":"13","author":"Algammal","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_3","first-page":"19688","article-title":"Methicillin-Resistant Staphylococcus aureus (MRSA): Burden of Disease and Control Challenges in Europe","volume":"15","author":"Becker","year":"2010","journal-title":"Eurosurveillance"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s11274-021-03146-8","article-title":"Prevalence and Multidrug Resistance in Salmonella enterica Typhimurium: An Overview in South East Asia","volume":"37","author":"Patra","year":"2021","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4103\/sjmms.sjmms_30_17","article-title":"Vibrio parahaemolyticus: A Review on Distribution, Pathogenesis, Virulence Determinants and Epidemiology","volume":"5","author":"Ghenem","year":"2017","journal-title":"Saudi J. Med. Med. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1080\/21645515.2018.1504717","article-title":"The Global Burden and Epidemiology of Invasive Non-Typhoidal Salmonella Infections","volume":"15","author":"Balasubramanian","year":"2019","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Kumar, V., Roy, S., Behera, B.K., Bossier, P., and Das, B.K. (2021). Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture. Toxins, 13.","DOI":"10.3390\/toxins13080524"},{"key":"ref_8","first-page":"48","article-title":"Vibrio parahaemolyticus\u2014An Emerging Foodborne Pathogen\u2014A Review","volume":"5","author":"Nelapati","year":"2011","journal-title":"Vet. World"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"a019778","DOI":"10.1101\/cshperspect.a019778","article-title":"The Candida Pathogenic Species Complex","volume":"4","author":"Turner","year":"2014","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/nyas.13739","article-title":"New Pathogens, New Tricks: Emerging, Drug-Resistant Fungal Pathogens and Future Prospects for Antifungal Therapeutics: Drug-Resistant Fungal Pathogens","volume":"1435","author":"Shapiro","year":"2019","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"103243","DOI":"10.1016\/j.fgb.2019.103243","article-title":"Candida auris and Multidrug Resistance: Defining the New Normal","volume":"131","author":"Lockhart","year":"2019","journal-title":"Fungal Genet. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1089\/mdr.2018.0319","article-title":"Multidrug-Resistant Bacteria and Alternative Methods to Control Them: An Overview","volume":"25","author":"Vivas","year":"2019","journal-title":"Microb. Drug Resist."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Raimundo, I., Silva, S., Costa, R., and Keller-Costa, T. (2018). Bioactive Secondary Metabolites from Octocoral-Associated Microbes\u2014New Chances for Blue Growth. Mar. Drugs, 16.","DOI":"10.3390\/md16120485"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"347","DOI":"10.4062\/biomolther.2016.067","article-title":"Marine Sponges as a Drug Treasure","volume":"24","author":"Anjum","year":"2016","journal-title":"Biomol. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"057","DOI":"10.4103\/2278-330X.126527","article-title":"Eribulin Drug Review","volume":"03","author":"Shetty","year":"2014","journal-title":"South Asian J. Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1039\/b808331m","article-title":"Development of Yondelis\u00ae (Trabectedin, ET-743). A Semisynthetic Process Solves the Supply Problem","volume":"26","author":"Cuevas","year":"2009","journal-title":"Nat. Prod. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s40168-018-0428-1","article-title":"The Sponge Holobiont in a Changing Ocean: From Microbes to Ecosystems","volume":"6","author":"Pita","year":"2018","journal-title":"Microbiome"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s40168-018-0431-6","article-title":"Host-Microbe Interactions in Octocoral Holobionts\u2014Recent Advances and Perspectives","volume":"6","author":"Allemand","year":"2018","journal-title":"Microbiome"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkab335","article-title":"AntiSMASH 6.0: Improving Cluster Detection and Comparison Capabilities","volume":"49","author":"Blin","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.3389\/fmicb.2017.02499","article-title":"Comparative Metagenomics Reveals the Distinctive Adaptive Features of the Spongia Officinalis Endosymbiotic Consortium","volume":"8","author":"Karimi","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s40168-021-01031-y","article-title":"Metagenomic Insights into the Taxonomy, Function, and Dysbiosis of Prokaryotic Communities in Octocorals","volume":"9","author":"Saraiva","year":"2021","journal-title":"Microbiome"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1111\/jam.14999","article-title":"The Sponges Hymeniacidon perlevis and Halichondria panicea Are Reservoirs of Antibiotic-producing Bacteria against Multi-drug Resistant Staphylococcus aureus","volume":"131","author":"Dechamps","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00367-22","DOI":"10.1128\/msystems.00367-22","article-title":"Methods and Strategies to Uncover Coral-Associated Microbial Dark Matter","volume":"7","author":"Schultz","year":"2022","journal-title":"mSystems"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1111\/1574-6941.12140","article-title":"Molecular Richness and Biotechnological Potential of Bacteria Cultured from Irciniidae Sponges in the North-East Atlantic","volume":"85","author":"Esteves","year":"2013","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1038\/s41598-019-38737-x","article-title":"Genomic Blueprints of Sponge-Prokaryote Symbiosis Are Shared by Low Abundant and Cultivatable Alphaproteobacteria","volume":"9","author":"Karimi","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Karimi, E., and Costa, R. (2020). Isolation and Genome Sequencing of 14 Spongia sp. Bacterial Associates Expands the Taxonomic and Functional Breadth of the Cultivatable Marine Sponge Microbiome. BioRxiv.","DOI":"10.1101\/2020.03.20.000216"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Keller-Costa, T., Eriksson, D., Gon\u00e7alves, J.M.S., Gomes, N.C.M., Lago-Lest\u00f3n, A., and Costa, R. (2017). The Gorgonian Coral Eunicella labiata Hosts a Distinct Prokaryotic Consortium Amenable to Cultivation. FEMS Microbiol. Ecol., 93.","DOI":"10.1093\/femsec\/fix143"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e00658-21","DOI":"10.1128\/MRA.00658-21","article-title":"Draft Genome Sequence of Vibrio chagasii 18LP, Isolated from Gilthead Seabream (Sparus aurata) Larvae Reared in Aquaculture","volume":"10","author":"Califano","year":"2021","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e00813-21","DOI":"10.1128\/MRA.00813-21","article-title":"Draft Genome Sequence of Vibrio jasicida 20LP, an Opportunistic Bacterium Isolated from Fish Larvae","volume":"10","author":"Califano","year":"2021","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e00593-15","DOI":"10.1128\/genomeA.00593-15","article-title":"Draft Genome Sequence of Aliivibrio fischeri Strain 5LC, a Bacterium Retrieved from Gilthead Sea Bream (Sparus aurata) Larvae Reared in Aquaculture","volume":"3","author":"Califano","year":"2015","journal-title":"Genome Announc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/1755-0998.13248","article-title":"Characterization of Putative Circular Plasmids in Sponge-associated Bacterial Communities Using a Selective Multiply-primed Rolling Circle Amplification","volume":"21","author":"Oliveira","year":"2021","journal-title":"Mol. Ecol. Resour."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"127183","DOI":"10.1016\/j.micres.2022.127183","article-title":"Assessing the Genomic Composition, Putative Ecological Relevance and Biotechnological Potential of Plasmids from Sponge Bacterial Symbionts","volume":"265","author":"Oliveira","year":"2022","journal-title":"Microbiol. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"D633","DOI":"10.1093\/nar\/gkt1244","article-title":"Ribosomal Database Project: Data and Tools for High Throughput RRNA Analysis","volume":"42","author":"Cole","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/aem.49.1.1-7.1985","article-title":"A New Medium for the Enumeration and Subculture of Bacteria from Potable Water","volume":"49","author":"Reasoner","year":"1985","journal-title":"Appl. Env. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1046\/j.1462-2920.2002.00352.x","article-title":"Cultivation of Globally Distributed Soil Bacteria from Phylogenetic Lineages Previously Only Detected in Cultivation-Independent Surveys: Cultivation of Soil Bacteria","volume":"4","author":"Sait","year":"2002","journal-title":"Environ. Microbiol."},{"key":"ref_36","unstructured":"C\u00facio, A.C. (2011). Molecular Exploration of Bacterial Communities Associated with Azooxanthellate Gorgonians in the Coast of Algarve, South Portugal. [Master\u2019s Thesis, UALG]."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"W282","DOI":"10.1093\/nar\/gky467","article-title":"The Microbial Genomes Atlas (MiGA) Webserver: Taxonomic and Gene Diversity Analysis of Archaea and Bacteria at the Whole Genome Level","volume":"46","author":"Gunturu","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/s41589-019-0400-9","article-title":"A Computational Framework to Explore Large-Scale Biosynthetic Diversity","volume":"16","author":"Mullowney","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1128\/EC.00414-06","article-title":"Development of a Highly Efficient Gene Targeting System Induced by Transient Repression of YKU80 Expression in Candida glabrata","volume":"6","author":"Ueno","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.biochi.2019.12.002","article-title":"Deletion of CDR1 Reveals Redox Regulation of Pleiotropic Drug Resistance in Candida glabrata","volume":"170","author":"Galkina","year":"2020","journal-title":"Biochimie"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jpha.2015.11.005","article-title":"Methods for in Vitro Evaluating Antimicrobial Activity: A Review","volume":"6","author":"Balouiri","year":"2016","journal-title":"J. Pharm. Anal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4002","DOI":"10.1111\/1462-2920.14747","article-title":"Comparative Genomics Reveals Complex Natural Product Biosynthesis Capacities and Carbon Metabolism across Host-associated and Free-living Aquimarina (Bacteroidetes, Flavobacteriaceae) Species","volume":"21","author":"Silva","year":"2019","journal-title":"Env. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Silva, S.G., Paula, P., da Silva, J.P., Mil-Homens, D., Teixeira, M.C., Fialho, A.M., Costa, R., and Keller-Costa, T. (2022). Insights into the Antimicrobial Activities and Metabolomes of Aquimarina (Flavobacteriaceae, Bacteroidetes) Species from the Rare Marine Biosphere. Mar. Drugs, 20.","DOI":"10.3390\/md20070423"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/s41589-019-0313-7","article-title":"Automated Structure Prediction of Trans-Acyltransferase Polyketide Synthase Products","volume":"15","author":"Helfrich","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e202115802","DOI":"10.1002\/anie.202115802","article-title":"Aquimarins, Peptide Antibiotics with Amino-Modified C-Termini from a Sponge-Derived Aquimarina sp. Bacterium","volume":"61","author":"Dieterich","year":"2021","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1007\/s10126-014-9575-y","article-title":"Application of Diffusion Growth Chambers for the Cultivation of Marine Sponge-Associated Bacteria","volume":"16","author":"Steinert","year":"2014","journal-title":"Mar. Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1016\/j.bjm.2016.07.010","article-title":"Antimicrobial Profile of Arthrobacter kerguelensis VL-RK_09 Isolated from Mango Orchards","volume":"47","author":"Munaganti","year":"2016","journal-title":"Braz. J. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1007\/s10482-018-1044-6","article-title":"Phylogeny and Bioactivity of Epiphytic Gram-Positive Bacteria Isolated from Three Co-Occurring Antarctic Macroalgae","volume":"111","author":"Alvarado","year":"2018","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1007\/s00300-020-02731-1","article-title":"Microbial Insights from Antarctic and Mediterranean Shallow-Water Bone-Eating Worms","volume":"43","author":"Hewitt","year":"2020","journal-title":"Polar Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1111\/j.1462-2920.2012.02780.x","article-title":"Deep-Sea Bacteria Enriched by Oil and Dispersant from the Deepwater Horizon Spill: Enrichment of Oil Degraders from Gulf of Mexico","volume":"14","author":"Baelum","year":"2012","journal-title":"Environ. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1039\/C8NP00052B","article-title":"Biosynthesis and Chemical Diversity of \u03b2-Lactone Natural Products","volume":"36","author":"Robinson","year":"2019","journal-title":"Nat. Prod. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.syapm.2017.11.004","article-title":"Parendozoicomonas haliclonae Gen. Nov. sp. Nov. Isolated from a Marine Sponge of the Genus Haliclona and Description of the Family Endozoicomonadaceae Fam. Nov. Comprising the Genera Endozoicomonas, Parendozoicomonas, and Kistimonas","volume":"41","author":"Bartz","year":"2018","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"40579","DOI":"10.1038\/srep40579","article-title":"Endozoicomonas Genomes Reveal Functional Adaptation and Plasticity in Bacterial Strains Symbiotically Associated with Diverse Marine Hosts","volume":"7","author":"Neave","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/s40168-022-01343-7","article-title":"Metagenomics-Resolved Genomics Provides Novel Insights into Chitin Turnover, Metabolic Specialization, and Niche Partitioning in the Octocoral Microbiome","volume":"10","author":"Kozma","year":"2022","journal-title":"Microbiome"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s40168-020-00970-2","article-title":"Functional Metagenomics Reveals Differential Chitin Degradation and Utilization Features across Free-Living and Host-Associated Marine Microbiomes","volume":"9","author":"Raimundo","year":"2021","journal-title":"Microbiome"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e01249-20","DOI":"10.1128\/mSystems.01249-20","article-title":"Insights into the Cultured Bacterial Fraction of Corals","volume":"6","author":"Sweet","year":"2021","journal-title":"mSystems"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1000194","DOI":"10.4172\/2155-9546.1000194","article-title":"The Use of Indigenous Probiotic Halomonas aquamarina and Shewanella algae for White Shrimp (Litopenaeus vannamei Boone) Hatchery Productivity in Zero Water Discharge System","volume":"04","author":"Suantika","year":"2013","journal-title":"J. Aquac. Res. Dev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jksus.2013.03.002","article-title":"Screening and Characterization of Antimicrobial Secondary Metabolites from Halomonas salifodinae MPM-TC and Its in Vivo Antiviral Influence on Indian White Shrimp Fenneropenaeus indicus against WSSV Challenge","volume":"25","author":"Velmurugan","year":"2013","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1038\/s41396-018-0323-6","article-title":"Marine Probiotics: Increasing Coral Resistance to Bleaching through Microbiome Manipulation","volume":"13","author":"Rosado","year":"2019","journal-title":"ISME J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"737925","DOI":"10.3389\/fmicb.2021.737925","article-title":"Bacteria Cultivated From Sponges and Bacteria Not Yet Cultivated From Sponges\u2014A Review","volume":"12","author":"Dat","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Offret, C., Desriac, F., Le Chevalier, P., Mounier, J., J\u00e9gou, C., and Fleury, Y. (2016). Spotlight on Antimicrobial Metabolites from the Marine Bacteria Pseudoalteromonas: Chemodiversity and Ecological Significance. Mar. Drugs, 14.","DOI":"10.3390\/md14070129"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e02604-20","DOI":"10.1128\/AEM.02604-20","article-title":"A Pseudoalteromonas Clade with Remarkable Biosynthetic Potential","volume":"87","author":"Chau","year":"2021","journal-title":"Appl. Env. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e00132-18","DOI":"10.1128\/genomeA.00132-18","article-title":"Draft Genome Sequence of Labrenzia sp. Strain EL143, a Coral-Associated Alphaproteobacterium with Versatile Symbiotic Living Capability and Strong Halogen Degradation Potential","volume":"6","author":"Rodrigues","year":"2018","journal-title":"Genome Announc."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/j.syapm.2005.05.010","article-title":"Stappia alba sp. Nov., Isolated from Mediterranean Oysters","volume":"28","author":"Pujalte","year":"2005","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1038\/s41429-019-0192-x","article-title":"Labrenzbactin from a Coral-Associated Bacterium Labrenzia sp.","volume":"72","author":"Zhou","year":"2019","journal-title":"J. Antibiot."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2561","DOI":"10.3389\/fmicb.2019.02561","article-title":"Genome of Labrenzia sp. PHM005 Reveals a Complete and Active Trans-AT PKS Gene Cluster for the Biosynthesis of Labrenzin","volume":"10","author":"Schleissner","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e00320-21","DOI":"10.1128\/MRA.00320-21","article-title":"The Roseibium album (Labrenzia alba) Genome Possesses Multiple Symbiosis Factors Possibly Underpinning Host-Microbe Relationships in the Marine Benthos","volume":"10","author":"Couceiro","year":"2021","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"5607","DOI":"10.1099\/ijsem.0.004332","article-title":"List of Prokaryotic Names with Standing in Nomenclature (LPSN) Moves to the DSMZ","volume":"70","author":"Parte","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_69","unstructured":"(2022, August 28). Bethesda (MD); National Library of Medicine (US); National Center for Biotechnology Information National Center for Biotechnology Information (NCBI)[Internet], Available online: https:\/\/www.ncbi.nlm.nih.gov\/."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"341","DOI":"10.3389\/fmicb.2017.00341","article-title":"Beneficial Microorganisms for Corals (BMC): Proposed Mechanisms for Coral Health and Resilience","volume":"8","author":"Peixoto","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/S1074-5521(03)00091-7","article-title":"Characterization of the Mupirocin Biosynthesis Gene Cluster from Pseudomonas Fluorescens NCIMB 10586","volume":"10","author":"Hothersall","year":"2003","journal-title":"Chem. Biol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1021\/np060361d","article-title":"Identification of the Putative Bryostatin Polyketide Synthase Gene Cluster from \u201cCandidatus Endobugula Sertula\u201d, the Uncultivated Microbial Symbiont of the Marine Bryozoan Bugula neritina","volume":"70","author":"Sudek","year":"2007","journal-title":"J. Nat. Prod."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"104533","DOI":"10.1016\/j.bioorg.2020.104533","article-title":"Chemical Mining of Heterotrophic Shewanella algae Reveals Anti-Infective Potential of Macrocyclic Polyketides against Multidrug-Resistant Pathogens","volume":"108","author":"Chakraborty","year":"2021","journal-title":"Bioorgan. Chem."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1093\/femsre\/fuz031","article-title":"The Shewanella Genus: Ubiquitous Organisms Sustaining and Preserving Aquatic Ecosystems","volume":"44","author":"Lemaire","year":"2020","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1080\/08927014.2015.1038705","article-title":"Enhanced Biofilm Formation and Melanin Synthesis by the Oyster Settlement-Promoting Shewanella colwelliana Is Related to Hydrophobic Surface and Simulated Intertidal Environment","volume":"31","author":"Mitra","year":"2015","journal-title":"Biofouling"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1007\/s12010-017-2453-0","article-title":"Sponge-Associated Bacteria Produce Non-Cytotoxic Melanin Which Protects Animal Cells from Photo-Toxicity","volume":"183","author":"Vijayan","year":"2017","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"C\u00e1mara-Ruiz, M., Balebona, M.C., Mori\u00f1igo, M.\u00c1., and Esteban, M.\u00c1. (2020). Probiotic Shewanella putrefaciens (SpPdp11) as a Fish Health Modulator: A Review. Microorganisms, 8.","DOI":"10.3390\/microorganisms8121990"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.aquaculture.2018.01.027","article-title":"In Vitro and in Vivo Potential Probiotic Activity of Bacillus subtilis and Shewanella algae for Use in Litopenaeus vannamei Rearing","volume":"488","author":"Interaminense","year":"2018","journal-title":"Aquaculture"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3629","DOI":"10.1111\/jam.15491","article-title":"Heterologous Expression of a Cryptic Gene Cluster from a Marine Proteobacterium Thalassomonas actiniarum Affords New Lanthipeptides Thalassomonasins A and B","volume":"132","author":"Thetsana","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1093\/ps\/82.4.640","article-title":"Alternatives to Antibiotics: Bacteriocins, Antimicrobial Peptides and Bacteriophages","volume":"82","author":"Joerger","year":"2003","journal-title":"Poult. Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1074\/jbc.REV119.006545","article-title":"The Manifold Roles of Microbial Ribosomal Peptide\u2013Based Natural Products in Physiology and Ecology","volume":"295","author":"Li","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00248-006-9165-2","article-title":"Bacteria of the Roseobacter Clade Show Potential for Secondary Metabolite Production","volume":"54","author":"Martens","year":"2007","journal-title":"Microb. Ecol."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"de Bruijn, F.J. (2016). Acyl-Homoserine Lactone-Based Quorum Sensing in Members of the Marine Bacterial Roseobacter Clade: Complex Cell-to-Cell Communication Controls Multiple Physiologies. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria, John Wiley & Sons, Inc.","DOI":"10.1002\/9781119004813"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"142","DOI":"10.15698\/mic2019.03.670","article-title":"Microevolution of the Pathogenic Yeasts Candida albicans and Candida glabrata during Antifungal Therapy and Host Infection","volume":"6","author":"Pais","year":"2019","journal-title":"Microb. Cell"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.cmi.2019.03.028","article-title":"Resistance of Candida to Azoles and Echinocandins Worldwide","volume":"25","author":"Pristov","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.micres.2015.06.007","article-title":"Broad Spectrum Antimicrobial Compounds from the Bacterium Exiguobacterium mexicanum MSSRFS9","volume":"178","author":"Shanthakumar","year":"2015","journal-title":"Microbiol. Res."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Romano, S., Jackson, S., Patry, S., and Dobson, A. (2018). Extending the \u201cOne Strain Many Compounds\u201d (OSMAC) Principle to Marine Microorganisms. Mar. Drugs, 16.","DOI":"10.3390\/md16070244"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"204","DOI":"10.3389\/fmicb.2017.00204","article-title":"Molecular Taxonomic Profiling of Bacterial Communities in a Gilthead Seabream (Sparus aurata) Hatchery","volume":"8","author":"Califano","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (ITOL) v5: An Online Tool for Phylogenetic Tree Display and Annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_91","unstructured":"(2021, August 26). Inkscape Project Inkscape. Available online: https:\/\/inkscape.org."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"e00855-16","DOI":"10.1128\/genomeA.00855-16","article-title":"Genomic Insights into Aquimarina sp. Strain EL33, a Bacterial Symbiont of the Gorgonian Coral Eunicella labiata","volume":"4","author":"Silva","year":"2016","journal-title":"Genome Announc."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"e01729-15","DOI":"10.1128\/genomeA.01729-15","article-title":"Draft Genome Sequence of Vibrio sp. Strain Evh12, a Bacterium Retrieved from the Gorgonian Coral Eunicella verrucosa","volume":"4","author":"Franco","year":"2016","journal-title":"Genome Announc."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"D751","DOI":"10.1093\/nar\/gkaa939","article-title":"The IMG\/M Data Management and Analysis System v.6.0: New Tools and Advanced Capabilities","volume":"49","author":"Chen","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"D36","DOI":"10.1093\/nar\/gks1195","article-title":"GenBank","volume":"41","author":"Benson","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1038\/nbt.4163","article-title":"KBase: The United States Department of Energy Systems Biology Knowledgebase","volume":"36","author":"Arkin","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Price, M.N., Dehal, P.S., and Arkin, A.P. (2010). FastTree 2\u2014Approximately Maximum-Likelihood Trees for Large Alignments. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009490"},{"key":"ref_99","first-page":"D454","article-title":"MIBiG 2.0: A Repository for Biosynthetic Gene Clusters of Known Function","volume":"48","author":"Kautsar","year":"2019","journal-title":"Nucleic Acids Res."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/21\/1\/34\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:48:50Z","timestamp":1760147330000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/21\/1\/34"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,30]]},"references-count":99,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["md21010034"],"URL":"https:\/\/doi.org\/10.3390\/md21010034","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,30]]}}}