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+UIDB\/50017\/2020+LA\/P\/0094\/2020"]}]},{"name":"LAQV-REQUIMTE","award":["LISBOA-01-0145-FEDER-402-022125"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-402-022125"]}]},{"name":"LAQV-REQUIMTE","award":["RECI\/QEQ-QFI\/0168\/2012"],"award-info":[{"award-number":["RECI\/QEQ-QFI\/0168\/2012"]}]},{"name":"LAQV-REQUIMTE","award":["CENTRO-07-CT62-FEDER-002012"],"award-info":[{"award-number":["CENTRO-07-CT62-FEDER-002012"]}]},{"name":"LAQV-REQUIMTE","award":["UID\/QUI\/00313\/2019"],"award-info":[{"award-number":["UID\/QUI\/00313\/2019"]}]},{"name":"LAQV-REQUIMTE","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Brown macroalgae are an important source of polysaccharides, mainly fucose-containing sulphated polysaccharides (FCSPs), associated with several biological activities. However, the structural diversity and structure\u2013function relationships for their bioactivities are still undisclosed. Thus, the aim of this work was to characterize the chemical structure of water-soluble Saccharina latissima polysaccharides and evaluate their immunostimulatory and hypocholesterolemic activities, helping to pinpoint a structure\u2013activity relationship. Alginate, laminarans (F1, neutral glucose-rich polysaccharides), and two fractions (F2 and F3) of FCSPs (negatively charged) were studied. Whereas F2 is rich in uronic acids (45 mol%) and fucose (29 mol%), F3 is rich in fucose (59 mol%) and galactose (21 mol%). These two fractions of FCSPs showed immunostimulatory activity on B lymphocytes, which could be associated with the presence of sulphate groups. Only F2 exhibited a significant effect in reductions in in vitro cholesterol\u2019s bioaccessibility attributed to the sequestration of bile salts. Therefore, S. latissima FCSPs were shown to have potential as immunostimulatory and hypocholesterolemic functional ingredients, where their content in uronic acids and sulphation seem to be relevant for the bioactive and healthy properties.<\/jats:p>","DOI":"10.3390\/md21030183","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T03:14:35Z","timestamp":1678936475000},"page":"183","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Water-Soluble Saccharina latissima Polysaccharides and Relation of Their Structural Characteristics with In Vitro Immunostimulatory and Hypocholesterolemic Activities"],"prefix":"10.3390","volume":"21","author":[{"given":"Ana S. P.","family":"Moreira","sequence":"first","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6305-9558","authenticated-orcid":false,"given":"Diana","family":"Gaspar","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2519-8979","authenticated-orcid":false,"given":"S\u00f3nia S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2965-4425","authenticated-orcid":false,"given":"Alexandra","family":"Correia","sequence":"additional","affiliation":[{"name":"i3S\u2014Institute for Research and Innovation in Health and IBMC\u2014Institute for Molecular and Cell Biology, University of Porto, 4200-135 Porto, Portugal"},{"name":"ICBAS\u2014Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"given":"Manuel","family":"Vilanova","sequence":"additional","affiliation":[{"name":"i3S\u2014Institute for Research and Innovation in Health and IBMC\u2014Institute for Molecular and Cell Biology, University of Porto, 4200-135 Porto, Portugal"},{"name":"ICBAS\u2014Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal"}]},{"given":"Marie-Mathilde","family":"Perrineau","sequence":"additional","affiliation":[{"name":"Scottish Association for Marine Sciences, Scottish Marine Institute, Oban PA37 1QA, UK"}]},{"given":"Philip D.","family":"Kerrison","sequence":"additional","affiliation":[{"name":"Scottish Association for Marine Sciences, Scottish Marine Institute, Oban PA37 1QA, UK"},{"name":"Hortimare BV, Altonstraat 25A, 1704 CC Heerhugowaard, The Netherlands"}]},{"given":"Claire M. M.","family":"Gachon","sequence":"additional","affiliation":[{"name":"Scottish Association for Marine Sciences, Scottish Marine Institute, Oban PA37 1QA, UK"},{"name":"Unit\u00e9 Mol\u00e9cules de Communication et Adaptation des Micro-Organismes (UMR 7245), Mus\u00e9um National d\u2019Histoire Naturelle, Centre National de la Recherche Scientifique (CNRS), 75005 Paris, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5357-3601","authenticated-orcid":false,"given":"Maria Ros\u00e1rio","family":"Domingues","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8898-6342","authenticated-orcid":false,"given":"Manuel A.","family":"Coimbra","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9498-2460","authenticated-orcid":false,"given":"Filipe M.","family":"Coreta-Gomes","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"},{"name":"CQC-IMS\u2014Coimbra Chemistry Centre, Institute of Molecular Sciences, University of Coimbra, 3004-535 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7217-2790","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Nunes","sequence":"additional","affiliation":[{"name":"CICECO\u2014Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"740054","DOI":"10.3389\/fmars.2021.740054","article-title":"Edible seaweeds: A potential novel source of bioactive metabolites and nutraceuticals with human health benefits","volume":"8","author":"Choudhary","year":"2021","journal-title":"Front. 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