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These may be obtained through extraction, which has conventionally been performed using hazardous organic solvents. To substitute such solvents with \u201cgreener\u201d alternatives, several technologies have been studied to increase their extraction potential. Distinct technologies utilize different principles to achieve such objective; while some aim at disrupting the cell walls of the microalgae, others target the extraction per se. While some methods have been utilized independently, several technologies have also been combined, which has proven to be an effective strategy. The current review focuses on the technologies explored in the last five years to extract or increase extraction yields of fatty acids from Nannochloropsis microalgae. Depending on the extraction efficacy of the different technologies, distinct types of lipids and\/or fatty acids are obtained accordingly. Moreover, the extraction efficiency may vary depending on the Nannochloropsis species. Hence, a case-by-case assessment must be conducted in order to ascertain the most suited technology, or tailor a specific one, to be applied to recover a particular fatty acid (or fatty acid class), namely PUFA, including eicosapentaenoic acid.<\/jats:p>","DOI":"10.3390\/md21060365","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T01:59:30Z","timestamp":1687226370000},"page":"365","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Extraction of Nannochloropsis Fatty Acids Using Different Green Technologies: The Current Path"],"prefix":"10.3390","volume":"21","author":[{"given":"S\u00e9rgio Cruz","family":"Sousa","sequence":"first","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"REQUIMTE\/LAQV\u2014Instituto Superior de Engenharia, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1430-9370","authenticated-orcid":false,"given":"Ana Cristina","family":"Freitas","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7883-2446","authenticated-orcid":false,"given":"Ana Maria","family":"Gomes","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5967-4200","authenticated-orcid":false,"given":"Ana P.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"REQUIMTE\/LAQV\u2014Instituto Superior de Engenharia, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4200-072 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.lfs.2013.08.002","article-title":"Health Applications of Bioactive Compounds from Marine Microalgae","volume":"93","author":"Morais","year":"2013","journal-title":"Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"103919","DOI":"10.1016\/j.jff.2020.103919","article-title":"Microalgae of the Genus Nannochloropsis: Chemical Composition and Functional Implications for Human Nutrition","volume":"68","author":"Zanella","year":"2020","journal-title":"J. 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