{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T21:54:32Z","timestamp":1768686872138,"version":"3.49.0"},"reference-count":66,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T00:00:00Z","timestamp":1699574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ALGALUP project","award":["ref: 0558-ALGALUP-6"],"award-info":[{"award-number":["ref: 0558-ALGALUP-6"]}]},{"name":"European Union\/ERDF, ESF, European Regional Development Fund ERDF under the Interreg V A Spain-Portugal (POCTEP) 2014\u20132020 program","award":["ref: 0558-ALGALUP-6"],"award-info":[{"award-number":["ref: 0558-ALGALUP-6"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Crude polysaccharides extracted from the Codium sp. and Osmundea sp. macroalgae collected in different seasons (winter, spring and summer) from the Galician and North Portugal coasts were characterised, aiming to support their biomedical application to wound healing. An increase in polysaccharides\u2019 sulphate content was registered from winter to summer, and higher values were obtained for Osmundea sp. In turn, the monosaccharide composition constantly changed with a decrease in glucose in Osmundea sp. from spring to winter. For Codium sp., a higher increase was noticed regarding glucose content in the Galician and Portugal coasts. Galactose was the major monosaccharide in all the samples, remaining stable in all seasons and collection sites. These results corroborate the sulphate content and antioxidant activity, since the Osmundea sp.-derived polysaccharides collected in summer exhibited higher scavenging radical ability. The biocompatibility and wound scratch assays revealed that the Osmundea sp. polysaccharide extracted from the Portugal coast in summer possessed more potential for promoting fibroblast migration. This study on seasonal variations of polysaccharides, sulphate content, monosaccharide composition and, consequently, biological properties provides practical guidance for determining the optimal season for algae harvest to standardise preparations of polysaccharides for the biomedical field.<\/jats:p>","DOI":"10.3390\/md21110589","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T02:46:47Z","timestamp":1699843607000},"page":"589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Chemical Composition of Macroalgae Polysaccharides from Galician and Portugal Coasts: Seasonal Variations and Biological Properties"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7274-0399","authenticated-orcid":false,"given":"S\u00f3nia P.","family":"Miguel","sequence":"first","affiliation":[{"name":"CPIRN-UDI\/IPG, Center for Potential and Innovation of Natural Resources, Polytechnic of Guarda, Av. Dr. Francisco S\u00e1 Carneiro, 50, 6300-559 Guarda, Portugal"},{"name":"CICS-UBI, Health Sciences Research Center, University of Beira Interior, Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"given":"Ca\u00edque","family":"D\u2019Angelo","sequence":"additional","affiliation":[{"name":"CPIRN-UDI\/IPG, Center for Potential and Innovation of Natural Resources, Polytechnic of Guarda, Av. Dr. Francisco S\u00e1 Carneiro, 50, 6300-559 Guarda, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6357-8115","authenticated-orcid":false,"given":"Maximiano P.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"CPIRN-UDI\/IPG, Center for Potential and Innovation of Natural Resources, Polytechnic of Guarda, Av. Dr. Francisco S\u00e1 Carneiro, 50, 6300-559 Guarda, Portugal"},{"name":"CICS-UBI, Health Sciences Research Center, University of Beira Interior, Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8143-1555","authenticated-orcid":false,"given":"Rog\u00e9rio","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"FibEnTech, Fiber Materials and Envornmental Technologies, University of Beira Interior, R. Marques Avila e Bolama, 6201-001 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6832-3082","authenticated-orcid":false,"given":"Paula","family":"Coutinho","sequence":"additional","affiliation":[{"name":"CPIRN-UDI\/IPG, Center for Potential and Innovation of Natural Resources, Polytechnic of Guarda, Av. Dr. Francisco S\u00e1 Carneiro, 50, 6300-559 Guarda, Portugal"},{"name":"CICS-UBI, Health Sciences Research Center, University of Beira Interior, Avenida Infante D. 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Biomedicines, 8.","DOI":"10.20944\/preprints202007.0284.v1"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/j.foodres.2016.11.016","article-title":"Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification","volume":"99","author":"Rajauria","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1016\/j.phytochem.2009.04.020","article-title":"Effect of season on the composition of bioactive polysaccharides from the brown seaweed saccharina longicruris","volume":"70","author":"Rioux","year":"2009","journal-title":"Phytochemistry"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"115852","DOI":"10.1016\/j.carbpol.2020.115852","article-title":"Composition and structure of cell wall ulvans recovered from ulva spp. Along the swedish west coast","volume":"233","author":"Nylander","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"58857","DOI":"10.1007\/s11356-021-12609-2","article-title":"Evaluation of seasonal variation and the optimization of reducing sugar extraction from ulva prolifera biomass using thermochemical method","volume":"28","author":"Dave","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.algal.2016.10.015","article-title":"The seasonal variation of fucoidan within three species of brown macroalgae","volume":"22","author":"Fletcher","year":"2017","journal-title":"Algal Res."},{"key":"ref_8","first-page":"833","article-title":"Seasonal variation in biopharmaceutical activity and fatty acid content of endemic fucus virsoides algae from adriatic sea","volume":"76","year":"2019","journal-title":"Acta Pol. Pharm.-Drug Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1007\/s10811-016-0995-0","article-title":"Novel rapid method for the characterisation of polymeric sugars from macroalgae","volume":"29","author":"Spicer","year":"2017","journal-title":"J. Appl. Phycol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"537202","DOI":"10.1155\/2013\/537202","article-title":"Analysis by vibrational spectroscopy of seaweed polysaccharides with potential use in food, pharmaceutical, and cosmetic industries","volume":"2013","author":"Pereira","year":"2013","journal-title":"Int. J. Carbohydr. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1016\/j.foodhyd.2011.02.009","article-title":"Ftir-atr spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds","volume":"25","year":"2011","journal-title":"Food Hydrocoll."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.foodchem.2015.03.057","article-title":"Chemical composition of red, brown and green macroalgae from buarcos bay in central west coast of portugal","volume":"183","author":"Rodrigues","year":"2015","journal-title":"Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1016\/j.foodhyd.2008.11.014","article-title":"Identification of selected seaweed polysaccharides (phycocolloids) by vibrational spectroscopy (ftir-atr and ft-raman)","volume":"23","author":"Pereira","year":"2009","journal-title":"Food Hydrocoll."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Figueroa, F.A., Abdala-D\u00edaz, R.T., P\u00e9rez, C., Casas-Arrojo, V., Nesic, A., Tapia, C., Dur\u00e1n, C., Valdes, O., Parra, C., and Bravo-Arrepol, G. (2022). Sulfated polysaccharide extracted from the green algae codium bernabei: Physicochemical characterization and antioxidant, anticoagulant and antitumor activity. Mar. Drugs, 20.","DOI":"10.3390\/md20070458"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Arunkumar, K., Sreena, K.S., Moosa, M., Mohan, G., and Raja, R. (2023). Cytotoxic characterization of optically negative codium fragile polysaccharide against hela and mcf cell lines. Bioact. Carbohydr. Diet. Fibre, 29.","DOI":"10.1016\/j.bcdf.2022.100341"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s12257-008-0102-8","article-title":"Partial characterization of extracellular polysaccharides produced by cyanobacterium arthrospira platensis","volume":"14","author":"Trabelsi","year":"2009","journal-title":"Biotechnol. Bioprocess Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3687","DOI":"10.1016\/j.ijbiomac.2020.08.206","article-title":"An ultrasonic-extracted arabinoglucan from tamarindus indica l. Pulp: A study on molecular and structural characterizations","volume":"164","author":"Guo","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Yada, R.Y. (2004). Proteins in Food Processing, Woodhead Publishing Ltd.","DOI":"10.1201\/9781439823309"},{"key":"ref_19","unstructured":"Barsanti, L., and Gualtieri, P. (2014). Algae: Anatomy, Biochemistry, and Biotechnology, CRC Press."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0168-3659(01)00451-5","article-title":"Long-circulating poly (ethylene glycol)\u2013poly (d, l-lactide) block copolymer micelles with modulated surface charge","volume":"77","author":"Yamamoto","year":"2001","journal-title":"J. Control. Release"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6235","DOI":"10.1111\/are.15485","article-title":"The effect of seasonality and geographic location on sulphated polysaccharides from brown algae","volume":"52","author":"Ptak","year":"2021","journal-title":"Aquac. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1007\/s10600-010-9507-7","article-title":"Seasonal variations of the composition and structural characteristics of polysaccharides from the brown alga costaria costata","volume":"45","author":"Imbs","year":"2009","journal-title":"Chem. Nat. Compd."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1134\/S1063074016040106","article-title":"Seasonal variations in the fucoidan content of brown algae from peter the great bay, sea of Japan","volume":"42","author":"Skriptsova","year":"2016","journal-title":"Russ. J. Mar. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1007\/s10126-011-9413-4","article-title":"Comparative study of polysaccharides from reproductive and sterile tissues of five brown seaweeds","volume":"14","author":"Skriptsova","year":"2012","journal-title":"Mar. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s10811-009-9438-5","article-title":"Monthly changes in the content and monosaccharide composition of fucoidan from undaria pinnatifida (laminariales, phaeophyta)","volume":"22","author":"Skriptsova","year":"2010","journal-title":"J. Appl. Phycol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1093\/glycob\/cwm014","article-title":"A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds","volume":"17","author":"Cumashi","year":"2007","journal-title":"Glycobiology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.2174\/0929867043364504","article-title":"Sulfated seaweed polysaccharides as antiviral agents","volume":"11","author":"Damonte","year":"2004","journal-title":"Curr. Med. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.biopha.2008.09.012","article-title":"Heparinoids algal and their anticoagulant, hemorrhagic activities and platelet aggregation","volume":"63","author":"Bezerra","year":"2009","journal-title":"Biomed. Pharmacother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.biopha.2009.03.005","article-title":"Biological activities of sulfated polysaccharides from tropical seaweeds","volume":"64","author":"Costa","year":"2010","journal-title":"Biomed. Pharmacother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"117830","DOI":"10.1016\/j.carbpol.2021.117830","article-title":"Effect of polysaccharide conformation on ultrafiltration separation performance","volume":"260","author":"Eder","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.carbpol.2007.01.009","article-title":"Characterization of polysaccharides extracted from brown seaweeds","volume":"69","author":"Rioux","year":"2007","journal-title":"Carbohydr. Polym."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"El-Sheekh, M.M., Bases, E., El Shafay, S.M., and El-shenody, R. (2023). Influence of seasonal variations on extract yield and antioxidant activities of some seaweed species. Proc. Natl. Acad. Sci. India Sect. B Biol. Sci., 1\u20139.","DOI":"10.1007\/s40011-023-01493-0"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0144-8617(88)90067-7","article-title":"Effects of seasons on the chemical structure and gel strength of gracilaria pseudoverrucosa agar (gracilariaceae, rhodophyta)","volume":"8","author":"Lahaye","year":"1988","journal-title":"Carbohydr. Polym."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.carbpol.2014.12.036","article-title":"Structural characterization and anticoagulant activity of a sulfated polysaccharide from the green alga codium divaricatum","volume":"121","author":"Li","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/S0008-6215(99)00206-2","article-title":"A new procedure for the separation of water-soluble polysaccharides from brown seaweeds","volume":"322","author":"Zvyagintseva","year":"1999","journal-title":"Carbohydr. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.ijbiomac.2021.03.193","article-title":"Degradation of codium cylindricum polysaccharides by h2o2-vc-ultrasonic and h2o2-fe2+-ultrasonic treatment: Structural characterization and antioxidant activity","volume":"182","author":"Yan","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1002\/jssc.202001140","article-title":"Analysis of monosaccharide composition of water-soluble polysaccharides from codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry","volume":"44","author":"Li","year":"2021","journal-title":"J. Sep. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.1007\/s10811-020-02183-4","article-title":"Concise review of osmundea pinnatifida (hudson) stackhouse","volume":"32","author":"Silva","year":"2020","journal-title":"J. Appl. Phycol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Rodrigues, D., Costa-Pinto, A.R., Sousa, S., Vasconcelos, M.W., Pintado, M.M., Pereira, L., Rocha-Santos, T.A., Costa, J.P.d., Silva, A., and Duarte, A.C. (2019). Sargassum muticum and osmundea pinnatifida enzymatic extracts: Chemical, structural, and cytotoxic characterization. Mar. Drugs, 17.","DOI":"10.3390\/md17040209"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1007\/s10811-022-02719-w","article-title":"Annual compositional variation in wild osmundea pinnatifida (hudson) stackhouse from the west coast of Scotland","volume":"34","author":"Biancacci","year":"2022","journal-title":"J. Appl. Phycol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.carbpol.2013.02.047","article-title":"Fucoidan from new zealand undaria pinnatifida: Monthly variations and determination of antioxidant activities","volume":"95","author":"Mak","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.ijbiomac.2007.10.003","article-title":"Antioxidant activity of sulfated polysaccharide fractions extracted from laminaria japonica","volume":"42","author":"Wang","year":"2008","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.carbpol.2010.10.062","article-title":"Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae","volume":"84","author":"Wijesekara","year":"2011","journal-title":"Carbohydr. Polym."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s10811-006-9121-z","article-title":"Antioxidant activities of sulfated polysaccharides from brown and red seaweeds","volume":"19","author":"Marques","year":"2007","journal-title":"J. Appl. Phycol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.ijbiomac.2005.10.008","article-title":"Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from ulva pertusa (chlorophyta) in vitro","volume":"37","author":"Qi","year":"2005","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2542950","DOI":"10.1155\/2017\/2542950","article-title":"Effect of content of sulfate groups in seaweed polysaccharides on antioxidant activity and repair effect of subcellular organelles in injured hk-2 cells","volume":"2017","author":"Ma","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.carbpol.2010.04.002","article-title":"Sulfated modification, characterization and structure\u2013antioxidant relationships of artemisia sphaerocephala polysaccharides","volume":"81","author":"Wang","year":"2010","journal-title":"Carbohydr. Polym."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Devi, K.P., Suganthy, N., Kesika, P., and Pandian, S.K. (2008). Bioprotective properties of seaweeds: In vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content. BMC Complement. Altern. Med., 8.","DOI":"10.1186\/1472-6882-8-38"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.aquaculture.2018.06.079","article-title":"Bioactivities of the edible brown seaweed, undaria pinnatifida: A review","volume":"495","author":"Wang","year":"2018","journal-title":"Aquaculture"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ecoenv.2018.05.024","article-title":"Anti-inflammatory potential of alginic acid from sargassum horneri against urban aerosol-induced inflammatory responses in keratinocytes and macrophages","volume":"160","author":"Fernando","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1111\/j.1461-0248.2005.00871.x","article-title":"The impacts of climate change in coastal marine systems","volume":"9","author":"Harley","year":"2006","journal-title":"Ecol. Lett."},{"key":"ref_52","first-page":"350","article-title":"Temporal changes in the seaweed flora in southern brazil and its potential causes","volume":"5","author":"Faveri","year":"2010","journal-title":"Panam. J. Aquat. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.ejar.2017.04.001","article-title":"Seasonal variation in the growth responses of some chlorophytic algal flora of the red sea","volume":"43","author":"Ansari","year":"2017","journal-title":"Egypt. J. Aquat. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4373","DOI":"10.3390\/molecules17044373","article-title":"Chemical characteristics and antioxidant properties of crude water soluble polysaccharides from four common edible mushrooms","volume":"17","author":"He","year":"2012","journal-title":"Molecules"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1016\/j.ijbiomac.2016.05.111","article-title":"Protective effects of polysaccharides from psidium guajava leaves against oxidative stresses","volume":"91","author":"Kim","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_56","unstructured":"Vavilala, S.L., and D\u2019Souza, J.S. (2015). Marine Algae extracts: Processes, Products, and Applications, Wiley."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2174\/1381612825666190618152133","article-title":"Bioactive algal-derived polysaccharides: Multi-functionalization, therapeutic potential and biomedical applications","volume":"25","author":"Muhamad","year":"2019","journal-title":"Curr. Pharm. Des."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.2174\/1381612825666190423160357","article-title":"Emerging biomedical applications of algal polysaccharides","volume":"25","author":"Rahmati","year":"2019","journal-title":"Curr. Pharm. Des."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"278","DOI":"10.4161\/biom.22947","article-title":"Marine algae sulfated polysaccharides for tissue engineering and drug delivery approaches","volume":"2","author":"Silva","year":"2012","journal-title":"Biomatter"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"116310","DOI":"10.1016\/j.carbpol.2020.116310","article-title":"Polysaccharides from gracilaria lemaneiformis promote the hacat keratinocytes wound healing by polarised and directional cell migration","volume":"241","author":"Veeraperumal","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"9212314","DOI":"10.1155\/2019\/9212314","article-title":"Effect of polysaccharides from bletilla striata on the healing of dermal wounds in mice","volume":"2019","author":"Zhang","year":"2019","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1042\/bj0840106","article-title":"A note on the determination of the ester sulphate content of sulphated polysaccharides","volume":"84","author":"Dodgson","year":"1962","journal-title":"Biochem. J."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Bhadja, P., Tan, C.-Y., Ouyang, J.-M., and Yu, K. (2016). Repair effect of seaweed polysaccharides with different contents of sulfate group and molecular weights on damaged hk-2 cells. Polymers, 8.","DOI":"10.3390\/polym8050188"},{"key":"ref_64","first-page":"1","article-title":"Determination of structural carbohydrates and lignin in biomass","volume":"1617","author":"Sluiter","year":"2008","journal-title":"Lab. Anal. Proced."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.ijbiomac.2018.10.041","article-title":"Production and characterization of electrospun silk fibroin based asymmetric membranes for wound dressing applications","volume":"121","author":"Miguel","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Alves, A., Miguel, S.P., Araujo, A.R., de Jes\u00fas Valle, M.J., S\u00e1nchez Navarro, A., Correia, I.J., Ribeiro, M.P., and Coutinho, P. (2020). Xanthan gum\u2013konjac glucomannan blend hydrogel for wound healing. Polymers, 12.","DOI":"10.3390\/polym12010099"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/21\/11\/589\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:21:06Z","timestamp":1760131266000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/21\/11\/589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,10]]},"references-count":66,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["md21110589"],"URL":"https:\/\/doi.org\/10.3390\/md21110589","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,10]]}}}