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This makes these organisms a putative source of commercially interesting compounds since adaptation provides different biochemical routes from those found in their terrestrial counterparts. In this work, the goal was the identification of a marine bacterium isolated from a sample taken at a shallow water hydrothermal vent and of its red product. Genomic, lipidomic, and biochemical approaches were used simultaneously, and the bacterium was identified as Serratia rubidaea. A high-throughput screening strategy was used to assess the best physico-chemical conditions permitting both cell growth and production of the red product. The fatty acid composition of the microbial cells was studied to assess adaptation at the lipid level under stressful conditions, whilst several state-of-the-art techniques, such as DSC, FTIR, NMR, and Ultra-High Resolution Qq-Time-of-Flight mass spectrometry, were used to characterize the structure of the pigment. We hypothesize that the pigment, which could be produced by the cells up to 62 \u00b0C, is prodigiosin linked to an aliphatic compound that acts as an anchor to keep it close to the cells in the marine environment.<\/jats:p>","DOI":"10.3390\/md21120599","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T12:12:13Z","timestamp":1700568733000},"page":"599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Isolation and Characterization of a Serratia rubidaea from a Shallow Water Hydrothermal Vent"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9722-1931","authenticated-orcid":false,"given":"Ricardo F. S.","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Bioengineering, iBB\u2014Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"},{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7909-637X","authenticated-orcid":false,"given":"Maria J.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Institute of Molecular Sciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3068-4920","authenticated-orcid":false,"given":"M. Concei\u00e7\u00e3o","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Institute of Molecular Sciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal"}]},{"given":"Maria C.","family":"Serra","sequence":"additional","affiliation":[{"name":"\u00c1rea Departamental de Engenharia Qu\u00edmica, Instituto Superior de Engenharia de Lisboa (ISEL), Rua Conselheiro Em\u00eddio Navarro, 1, 1959-007 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8142-3835","authenticated-orcid":false,"given":"Carla C. C. R.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, iBB\u2014Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"},{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rodrigues, C.J.C., Pereira, R.F.S., Fernandes, P., Cabral, J.M.S., and de Carvalho, C.C.C.R. (2017). Cultivation-based strategies to find efficient marine biocatalysts. Biotechnol. J., 12.","DOI":"10.1002\/biot.201700036"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.dyepig.2014.06.011","article-title":"Marine natural pigments: Chemistry, distribution and analysis","volume":"111","author":"Pereira","year":"2014","journal-title":"Dye. Pigment."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1039\/C4DT02756F","article-title":"Porphyrins as excellent dyes for dye-sensitized solar cells: Recent developments and insights","volume":"44","author":"Higashino","year":"2015","journal-title":"Dalt. Trans."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"81591","DOI":"10.18632\/oncotarget.20189","article-title":"Porphyrin photosensitizers in photodynamic therapy and its applications","volume":"8","author":"Kou","year":"2017","journal-title":"Oncotarget"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3390\/md202073","article-title":"Drugs and cosmetics from the Sea","volume":"2","author":"Kijjoa","year":"2004","journal-title":"Mar. Drugs"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1055\/s-0034-1383001","article-title":"Exploring marine resources for bioactive compounds","volume":"80","author":"Kiuru","year":"2014","journal-title":"Planta Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"705","DOI":"10.3390\/md8030705","article-title":"Production of metabolites as bacterial responses to the marine environment","volume":"8","author":"Fernandes","year":"2010","journal-title":"Mar. Drugs"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.marenvres.2016.05.002","article-title":"Marine microorganisms as a promising and sustainable source of bioactive molecules","volume":"128","author":"Romano","year":"2017","journal-title":"Mar. Environ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/0167-7799(94)90029-9","article-title":"Mechanisms of resistance of whole cells to toxic organic solvents","volume":"12","author":"Heipieper","year":"1994","journal-title":"Trends Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0304-4157(96)00010-X","article-title":"Adaptation mechanism of microorganisms to toxic effects of organic solvents on membranes","volume":"1286","author":"Weber","year":"1996","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1006\/bbrc.1997.7186","article-title":"A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans","volume":"237","author":"Kawauchi","year":"1997","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7818","DOI":"10.1021\/acs.chemrev.6b00024","article-title":"Structure, chemical synthesis, and biosynthesis of prodiginine natural products","volume":"116","author":"Hu","year":"2016","journal-title":"Chem. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1038\/nrmicro1531","article-title":"The biosynthesis and regulation of bacterila prodiginines","volume":"4","author":"Williamson","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ponjavic, M., Malagurski, I., Lazic, J., Jeremic, S., Pavlovic, V., Prlainovic, N., Maksimovic, V., Cosovic, V., Atanase, L.I., and Freitas, F. (2023). Advancing PHBV biomedical potential with the incorporation of bacterial biopigment prodigiosin. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24031906"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.2174\/092986710791331103","article-title":"New insights on the antitumoral properties of prodiginines","volume":"17","author":"Vinas","year":"2010","journal-title":"Curr. Med. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"173465","DOI":"10.1016\/j.ejphar.2020.173465","article-title":"Survivin modulation in the antimelanoma activity of prodiginines","volume":"888","author":"Branco","year":"2020","journal-title":"Eur. J. Pharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7066","DOI":"10.1093\/nar\/gki1016","article-title":"Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent","volume":"33","author":"Jeong","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1002\/jobm.202100101","article-title":"High-level production of microbial prodigiosin: A review","volume":"61","author":"Han","year":"2021","journal-title":"J. Basic Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jiph.2022.11.020","article-title":"A rare occurrence of Serratia rubidaea in a patient with ear discharge","volume":"16","author":"Karnaker","year":"2023","journal-title":"J. Infect. Public Health"},{"key":"ref_20","unstructured":"Kunitsky, C., Osterhout, G., and Sasser, M. (2006). Encyclopedia of Rapid Microbiological Methods."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"de Carvalho, C.C.C.R., and Caramujo, M.J. (2018). The various roles of fatty acids. Molecules, 23.","DOI":"10.3390\/molecules23102583"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.btre.2017.04.001","article-title":"Optimization of prodigiosin production by Serratia marcescens using crude glycerol and enhancing production using gamma radiation","volume":"14","author":"Elkenawy","year":"2017","journal-title":"Biotechnol. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4442","DOI":"10.1128\/AEM.00722-15","article-title":"Rich medium composition affects Escherichia coli survival, glycation, and mutation frequency during long-term batch culture","volume":"81","author":"Kram","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/jb.71.1.115-120.1956","article-title":"Studies on pigmentation of Serratia marcescens. I. Spectral and paper chromatographic properties of prodigiosin","volume":"71","author":"Williams","year":"1956","journal-title":"J. Bacteriol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"264","DOI":"10.4319\/lo.1967.12.2.0264","article-title":"Growth of marine bacteria at limiting concentrations of organic carbon in seawater","volume":"12","author":"Jannasch","year":"1967","journal-title":"Limnol. Oceanogr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1146\/annurev-marine-122414-033938","article-title":"Growth rates of microbes in the oceans","volume":"8","author":"Kirchman","year":"2016","journal-title":"Ann. Rev. Mar. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.procbio.2011.11.020","article-title":"Anchoring high-throughput screening methods to scale-up bioproduction of siderophores","volume":"47","author":"Marques","year":"2012","journal-title":"Process Biochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1007\/s12665-010-0877-8","article-title":"Chemistry of the Ferraria thermal water, S. Miguel Island, Azores: Mixing and precipitation processes","volume":"64","author":"Carvalho","year":"2011","journal-title":"Environ. Earth Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"43","DOI":"10.21307\/pjm-2019-005","article-title":"Thermoregulation of prodigiosin biosynthesis by Serratia marcescens is controlled at the transcriptional level and requires HexS","volume":"68","author":"Romanowski","year":"2019","journal-title":"Pol. J. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Sun, Y., Wang, L., Pan, X., Osire, T., Fang, H., Zhang, H., Yang, S.-T., Yang, T., and Rao, Z. (2020). Improved prodigiosin production by relieving CpxR temperature-sensitive inhibition. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00344"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1111\/1758-2229.12306","article-title":"Extraordinary solute-stress tolerance contributes to the environmental tenacity of mycobacteria","volume":"7","author":"Santos","year":"2015","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4776","DOI":"10.1016\/j.jmb.2016.08.013","article-title":"Homeoviscous adaptation and the regulation of membrane lipids","volume":"428","author":"Ernst","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/0307-4412(91)90103-F","article-title":"Phase transitions in phospholipid bilayers: Lateral phase separations play vital roles in biomembranes","volume":"19","author":"Eze","year":"1991","journal-title":"Biochem. Educ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.resmic.2011.11.003","article-title":"Adaptation of Rhodococcus erythropolis cells for growth and bioremediation under extreme conditions","volume":"163","year":"2012","journal-title":"Res. Microbiol."},{"key":"ref_35","first-page":"429","article-title":"Cyclopropane ring formation in membrane lipids of bacteria","volume":"61","author":"Grogan","year":"1997","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1128\/AEM.02270-06","article-title":"Bacterial genes responsible for the biosynthesis of eicosapentaenoic and docosahexaenoic acids and their heterologous expression","volume":"73","author":"Okuyama","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1099\/13500872-145-4-767","article-title":"Polyunsaturated fatty acids in marine bacteria\u2014A dogma rewritten","volume":"145","author":"Russell","year":"1999","journal-title":"Microbiology"},{"key":"ref_38","first-page":"5599","article-title":"Rapid adaptation of Rhodococcus erythropolis cells to salt stress by synthesizing polyunsaturated fatty acids","volume":"98","author":"Marques","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"16240","DOI":"10.1038\/s41598-018-34264-3","article-title":"Omega-3 polyunsaturated fatty acids do not fluidify bilayers in the liquid-crystalline state","volume":"8","author":"Varela","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1186\/s40064-015-1476-7","article-title":"Alteration of zeta potential and membrane permeability in bacteria: A study with cationic agents","volume":"4","author":"Halder","year":"2015","journal-title":"Springerplus"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00792-017-0939-x","article-title":"Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure","volume":"21","author":"Siliakus","year":"2017","journal-title":"Extremophiles"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/nrmicro2549","article-title":"Molecular aspects of bacterial pH sensing and homeostasis","volume":"9","author":"Krulwich","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.oceano.2015.07.003","article-title":"Ocean acidification and marine microorganisms: Responses and consequences","volume":"57","author":"Das","year":"2015","journal-title":"Oceanologia"},{"key":"ref_44","unstructured":"Uddin, J. (2012). The Use of Spectrophotometry UV-Vis for the Study of Porphyrins, Intech."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1042\/bj0460220","article-title":"The biosynthesis of prodigiosin, the tripyrrylmethene pigment from Bacillus prodigiosus (Serratia marcescens)","volume":"46","author":"Hubbard","year":"1950","journal-title":"Biochem. J."},{"key":"ref_46","unstructured":"Williams, R.H. (1965). The Identification of Prodigiosin and Similar Compounds. [Ph.D. Thesis, Iowa State University of Sciences and Technology]."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5393","DOI":"10.1007\/s13197-015-1740-4","article-title":"Prodigiosin and its potential applications","volume":"52","author":"Darshan","year":"2013","journal-title":"J. Food Sci. Technol."},{"key":"ref_48","unstructured":"So, P.T., and Dong, C.Y. (2001). Encyclopedia of Life Sciences, Macmillan Publishers."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s13568-016-0222-z","article-title":"Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death","volume":"6","author":"Darshan","year":"2016","journal-title":"AMB Express"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.trac.2004.11.021","article-title":"Metabolomics: Current analytical platforms and methodologies","volume":"24","author":"Dunn","year":"2005","journal-title":"Trends Anal. Chem."},{"key":"ref_51","first-page":"595","article-title":"Natural products research and metabolomics","volume":"Volume 9","author":"Liu","year":"2010","journal-title":"Comprehensive Natural Products II: Chemistry and Biology"},{"key":"ref_52","unstructured":"Silverstein, R.M., Webster, F.X., and Kiemle, D.J. (2005). Spectrometric Identification of Organic Compounds, John Wiley & Sons, Inc.. [7th ed.]."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"65","DOI":"10.5194\/cp-7-65-2011","article-title":"Application of Fourier Transform Infrared Spectroscopy (FTIR) for assessing biogenic silica sample purity in geochemical analyses and palaeoenvironmental research","volume":"7","author":"Swann","year":"2011","journal-title":"Clim. Past"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.saa.2017.08.069","article-title":"Optical activity of Co-porphyrin in the light of IR and Raman spectroscopy: A critical DFT investigation","volume":"190","author":"Roy","year":"2018","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.dyepig.2016.07.018","article-title":"The pink pigment prodigiosin: Vibrational spectroscopy and DFT calculations","volume":"134","author":"Varnali","year":"2016","journal-title":"Dye. Pigment."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"14706","DOI":"10.1038\/s41598-020-71157-w","article-title":"Prodigiosin\/PU-H71 as a novel potential combined therapy for triple negative breast cancer (TNBC): Preclinical insights","volume":"10","author":"Anwar","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1128\/jb.113.1.122-132.1973","article-title":"Porphyrin-accumulating mutants of Escherichia coli","volume":"113","author":"Cox","year":"1973","journal-title":"J. Bacteriol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Domr\u00f6se, A., Klein, A.S., Hage-H\u00fclsmann, J., Thies, S., Svensson, V., Classen, T., Pietruszka, J., Jaeger, K.E., Drepper, T., and Loeschcke, A. (2015). Efficient recombinant production of prodigiosin in Pseudomonas putida. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00972"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1365-2958.2005.04602.x","article-title":"Biosynthesis of the red antibiotic, prodigiosin, in Serratia: Identification of a novel 2-methyl-3-n-amyl- pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Strepto","volume":"56","author":"Williamson","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref_60","unstructured":"Nakamura, K., and Kitamura, K. (1981). Process for Preparation of Prodigiosin. (4266028A), U.S. Patent."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1007\/s11095-005-7626-9","article-title":"Quantification of crystallinity in substantially amorphous materials by synchrotron X-ray powder diffractometry","volume":"22","author":"Nunes","year":"2005","journal-title":"Pharm. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1021\/np050255w","article-title":"Metabolomics reviewed: A new \u201comics\u201d platform technology for systems biology and implications for natural products research","volume":"68","author":"Rochfort","year":"2005","journal-title":"J. Nat. Prod."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Vijay, D., Alshamsi, N.S., Moussa, Z., and Akhtar, M.K. (2022). Extraction of the Anticancer and Antimicrobial Agent, Prodigiosin, from Vibrio gazogenes PB1 and Its Identification by 1D and 2D NMR. Molecules, 27.","DOI":"10.3390\/molecules27186030"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1007\/s00203-018-1508-0","article-title":"Prodigiosin pigment of Serratia marcescens is associated with increased biomass production","volume":"200","author":"Haddix","year":"2018","journal-title":"Arch. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Pereira, R.F.S., and de Carvalho, C.C.C.R. (2021). Optimization of multiparameters for increased yields of cytochrome B5 in bioreactors. Molecules, 26.","DOI":"10.3390\/molecules26144148"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.bej.2014.11.017","article-title":"Effect of carbon sources on lipid accumulation in Rhodococcus cells","volume":"94","author":"Cortes","year":"2015","journal-title":"Biochem. Eng. J."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/21\/12\/599\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:26:05Z","timestamp":1760131565000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/21\/12\/599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,21]]},"references-count":66,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["md21120599"],"URL":"https:\/\/doi.org\/10.3390\/md21120599","relation":{},"ISSN":["1660-3397"],"issn-type":[{"type":"electronic","value":"1660-3397"}],"subject":[],"published":{"date-parts":[[2023,11,21]]}}}