{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T06:42:51Z","timestamp":1774420971698,"version":"3.50.1"},"reference-count":75,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pacto da Bioeconomia azul","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]},{"name":"Next Generation EU European Fund","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]},{"name":"Portuguese Recovery and Resilience Plan","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The complex plant cell wall heteropolysaccharide xylan, and its breakdown products xylo-oligosaccharides and xylose, are value-added compounds with a plethora of potential applications in diverse areas. They are nonetheless currently poorly exploited, with a major bottleneck being the unavailability of efficient, low-cost, high-yield production processes. The major objective of the present study is to identify and characterise a high-yield process for the preparation of highly pure xylan\/XOS products from the macroalga Palmaria palmata. Currently, most xylan is extracted from land-sourced lignocellulosic feedstocks, but we take advantage of the high xylan content, xylan aqueous solubility, lignin-free nature, weakly linked cell wall matrix, and sustainability of the macroalga to identify a simple, sustainable, high-yield, novel-xylan-structure extraction process. This is composed of five steps: alga oven drying, milling, aqueous extraction, centrifugation, and dialysis, and we show that the alga preservation step plays a critical role in component extractability, with oven drying at high temperatures, ~100 \u00b0C, enhancing the subsequent aqueous extraction process, and providing for xylan yields as high as 80% of a highly pure (~90%) xylan product. The process developed herein and the insights gained will promote a greater availability of these bioactive compounds and open up their application potential.<\/jats:p>","DOI":"10.3390\/md23080302","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T09:31:45Z","timestamp":1753781505000},"page":"302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Simplified, High Yielding Extraction of Xylan\/Xylo-Oligosaccharides from Palmaria palmata: The Importance of the Algae Preservation Treatment"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6151-8186","authenticated-orcid":false,"given":"Diogo","family":"Coelho","sequence":"first","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA)\/Aquatic Research Network (ARNET), Department of Biology, University of Minho, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Diogo F\u00e9lix","family":"Costa","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA)\/Aquatic Research Network (ARNET), Department of Biology, University of Minho, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, 4710-057 Braga, Portugal"}]},{"given":"M\u00e1rio","family":"Barroca","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA)\/Aquatic Research Network (ARNET), Department of Biology, University of Minho, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1336-9993","authenticated-orcid":false,"given":"Sara Alexandra","family":"Cunha","sequence":"additional","affiliation":[{"name":"Universidade Cat\u00f3lica Portuguesa, CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0760-3184","authenticated-orcid":false,"given":"Maria Manuela","family":"Pintado","sequence":"additional","affiliation":[{"name":"Universidade Cat\u00f3lica Portuguesa, CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7630-3185","authenticated-orcid":false,"given":"Helena","family":"Abreu","sequence":"additional","affiliation":[{"name":"Independent Seaweed Consultant, 3870-322 Murtosa, Portugal"}]},{"given":"Margarida","family":"Martins","sequence":"additional","affiliation":[{"name":"ALGAplus, 3830-352 \u00cdlhavo, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4167-973X","authenticated-orcid":false,"given":"Tony","family":"Collins","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA)\/Aquatic Research Network (ARNET), Department of Biology, University of Minho, 4710-057 Braga, Portugal"},{"name":"Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100101","DOI":"10.1016\/j.tcsw.2023.100101","article-title":"An Update on Xylan Structure, Biosynthesis, and Potential Commercial Applications","volume":"9","author":"Curry","year":"2023","journal-title":"Cell Surf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1111\/j.1365-313X.2008.03729.x","article-title":"Characterization of IRX10 and IRX10-like Reveals an Essential Role in Glucuronoxylan Biosynthesis in Arabidopsis","volume":"57","author":"Brown","year":"2009","journal-title":"Plant J."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mendon\u00e7a, M., Barroca, M., and Collins, T. (2023). Endo-1,4-\u03b2-Xylanase-Containing Glycoside Hydrolase Families: Characteristics, Singularities and Similarities. Biotechnol. Adv., 65.","DOI":"10.1016\/j.biotechadv.2023.108148"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hsieh, Y.S.Y., and Harris, P.J. (2019). Xylans of Red and Green Algae: What Is Known about Their Structures and How They Are Synthesised?. Polymers, 11.","DOI":"10.3390\/polym11020354"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Sarkar, P., Bandyopadhyay, T.K., Gopikrishna, K., Nath Tiwari, O., Bhunia, B., and Muthuraj, M. (2024). Algal Carbohydrates: Sources, Biosynthetic Pathway, Production, and Applications. Bioresour. Technol., 413.","DOI":"10.1016\/j.biortech.2024.131489"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1016\/j.biotechadv.2016.09.001","article-title":"Towards Enzymatic Breakdown of Complex Plant Xylan Structures: State of the Art","volume":"34","author":"Biely","year":"2016","journal-title":"Biotechnol. Adv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.femsre.2004.06.005","article-title":"Xylanases, Xylanase Families and Extremophilic Xylanases","volume":"29","author":"Collins","year":"2005","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.carbpol.2017.09.064","article-title":"Bio-Based Products from Xylan: A Review","volume":"179","author":"Naidu","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Verbeek, J. (2012). Xylan, a Promising Hemicellulose for Pharmaceutical Use, Products and Applications of Biopolymers. Products and Application of Biopolymers, InTech.","DOI":"10.5772\/1802"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Smith, M.M., and Melrose, J. (2022). Xylan Prebiotics and the Gut Mcrobiome Promote Health and Wellbeing: Potential Novel Roles for Pentosan Polysulfate. Pharmaceuticals, 15.","DOI":"10.3390\/ph15091151"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wang, Z., Bai, Y., Pi, Y., Gerrits, W.J.J., de Vries, S., Shang, L., Tao, S., Zhang, S., Han, D., and Zhu, Z. (2021). Xylan Alleviates Dietary Fiber Deprivation-Induced Dysbiosis by Selectively Promoting Bifidobacterium Pseudocatenulatum in Pigs. Microbiome, 9.","DOI":"10.1186\/s40168-021-01175-x"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1017\/jns.2020.14","article-title":"The Role of Oligosaccharides and Polysaccharides of Xylan and Mannan in Gut Health of Monogastric Animals","volume":"9","author":"Tiwari","year":"2020","journal-title":"J. Nutr. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2174\/0113894501285058240203094846","article-title":"Recent Advances in Biomedical Applications of Mannans and Xylans","volume":"25","author":"Teli","year":"2024","journal-title":"Curr. Drug Targets"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.ejpb.2020.04.016","article-title":"Xylan in Drug Delivery: A Review of Its Engineered Structures and Biomedical Applications","volume":"151","year":"2020","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1002\/pat.3767","article-title":"Xylan Hemicellulose Improves Chitosan Hydrogel for Bone Tissue Regeneration","volume":"27","author":"Bush","year":"2016","journal-title":"Polym. Adv. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.msec.2012.12.032","article-title":"Xylan Polysaccharides Fabricated into Nanofibrous Substrate for Myocardial Infarction","volume":"33","author":"Venugopal","year":"2013","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"119772","DOI":"10.1016\/j.carbpol.2022.119772","article-title":"Engineering a Semi-Interpenetrating Constructed Xylan-Based Hydrogel with Superior Compressive Strength, Resilience, and Creep Recovery Abilities","volume":"294","author":"Han","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1016\/j.carbpol.2012.11.079","article-title":"Preparation of Xylan Citrate\u2014A Potential Adsorbent for Industrial Wastewater Treatment","volume":"92","author":"Shuaiyang","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.carbpol.2014.11.013","article-title":"Preparation and Adsorption Property of Xylan\/Poly(Acrylic Acid) Magnetic Nanocomposite Hydrogel Adsorbent","volume":"118","author":"Sun","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"122112","DOI":"10.1016\/j.carbpol.2024.122112","article-title":"Wood Inspired Biobased Nanocomposite Films Composed of Xylans, Lignosulfonates and Cellulose Nanofibers for Active Food Packaging","volume":"337","author":"Silva","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e202401877","DOI":"10.1002\/cssc.202401877","article-title":"Xylose Acetals\u2014A New Class of Sustainable Solvents and Their Application in Enzymatic Polycondensation","volume":"18","author":"Komarova","year":"2024","journal-title":"ChemSusChem"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.carbpol.2016.03.036","article-title":"Synthesis and Characterization of Carboxymethylated Xylan and Its Application as a Dispersant","volume":"146","author":"Konduri","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Josey, D.C., Yadavalli, N.S., Moore, J.C., Pe\u00f1a, M.J., Minko, S., and Urbanowicz, B.R. (2024). Valorization of Hemicellulose Waste Streams for Moisture Barrier Coatings and Hydrophobic Films. Biotechnol. Sus. Mater., 1.","DOI":"10.1186\/s44316-024-00009-8"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"35133","DOI":"10.1074\/jbc.M204517200","article-title":"A Novel Family 8 Xylanase, Functional and Physicochemical Characterization","volume":"277","author":"Collins","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.enzmictec.2016.10.011","article-title":"Deciphering the Factors Defining the pH-Dependence of a Commercial Glycoside Hydrolase Family 8 Enzyme","volume":"96","author":"Barroca","year":"2017","journal-title":"Enzyme Microb. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.3945\/jn.108.094367","article-title":"Structurally Different Wheat-Derived Arabinoxylooligosaccharides Have Different Prebiotic and Fermentation Properties in Rats","volume":"138","author":"Swennen","year":"2008","journal-title":"J. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.foodchem.2008.06.039","article-title":"Heat and PH Stability of Prebiotic Arabinoxylooligosaccharides, Xylooligosaccharides and Fructooligosaccharides","volume":"112","author":"Courtin","year":"2009","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Deng, J., Yun, J., Gu, Y., Yan, B., Yin, B., and Huang, C. (2023). Evaluating the in Vitro and in Vivo Prebiotic Effects of Different Xylo-Oligosaccharides Obtained from Bamboo Shoots by Hydrothermal Pretreatment Combined with Endo-Xylanase Hydrolysis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241713422"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Batsalova, T., Georgiev, Y., Moten, D., Teneva, I., and Dzhambazov, B. (2022). Natural Xylooligosaccharides Exert Antitumor Activity via Modulation of Cellular Antioxidant State and TLR4. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810430"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"139761","DOI":"10.1016\/j.foodchem.2024.139761","article-title":"Structure-Antioxidant Activity Relationship of Xylooligosaccharides Obtained from Carboxyl-Reduced Glucuronoarabinoxylans from Bamboo Shoots","volume":"455","author":"Alvarez","year":"2024","journal-title":"Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"skaf050","DOI":"10.1093\/jas\/skaf050","article-title":"Xylo-Oligosaccharides Enhance Intestinal and Thymic Immunity by Modulating Pyroptosis, Gut Microbiota, and Th17\/Treg Immune Response in Lipopolysaccharide-Challenged Piglets","volume":"103","author":"Sun","year":"2025","journal-title":"J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Sun, Z., Yue, Z., Liu, E., Li, X., and Li, C. (2022). Assessment of the Bifidogenic and Antibacterial Activities of Xylooligosaccharide. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.858949"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3177\/jnsv.54.396","article-title":"Effects of Xylooligosaccharides in Type 2 Diabetes Mellitus","volume":"54","author":"Sheu","year":"2008","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Li, C., Li, D., Liu, H., Zhang, N., Dang, L., Wang, M., Tian, H., Jha, R., and Li, C. (2024). Synbiotic Supplementation with Xylooligosaccharide Derived Probiotic Lactobacillus gasseri and Prebiotic Mixture Exerts Antidiabetic Effects via Collaborative Action. Food Biosci., 61.","DOI":"10.1016\/j.fbio.2024.104948"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1007\/s10068-015-0124-x","article-title":"Relative Sweetness, Sweetness Quality, and Temporal Profile of Xylooligosaccharides and Luo Han Guo (Siraitia grosvenorii) Extract","volume":"24","author":"Kim","year":"2015","journal-title":"Food. Sci. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1039\/D1SE00927C","article-title":"Valorisation of Xylose to Renewable Fuels and Chemicals, an Essential Step in Augmenting the Commercial Viability of Lignocellulosic Biorefineries","volume":"6","author":"Narisetty","year":"2022","journal-title":"Sustain. Energy Fuels"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4464","DOI":"10.1039\/D4GC06578F","article-title":"Sustainable Bioplastics Build on D-Xylose Cores: From Backup to the Center Stage","volume":"27","author":"Dai","year":"2025","journal-title":"Green Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10971","DOI":"10.1002\/slct.201801315","article-title":"Conversion of Fructose and Xylose into Platform Chemicals Using Organo-functionalized Mesoporous Material","volume":"3","author":"Kalita","year":"2018","journal-title":"ChemistrySelect"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"120381","DOI":"10.1016\/j.carbpol.2022.120381","article-title":"Xylan Extraction from Hardwoods by Alkaline Pretreatment for Xylooligosaccharide Production: A Detailed Fractionation Analysis","volume":"302","author":"Salazar","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Scapini, T., dos Santos, M.S.N., Bonatto, C., Wancura, J.H.C., Mulinari, J., Camargo, A.F., Klanovicz, N., Zabot, G.L., Tres, M.V., and Fongaro, G. (2021). Hydrothermal Pretreatment of Lignocellulosic Biomass for Hemicellulose Recovery. Bioresour. Technol., 342.","DOI":"10.1016\/j.biortech.2021.126033"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"21229","DOI":"10.1007\/s13399-023-04383-7","article-title":"Green Extraction of Xylan Hemicellulose from Wheat Straw","volume":"14","author":"Dafchahi","year":"2024","journal-title":"Biomass Convers. Biorefin."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.jbiosc.2021.01.009","article-title":"Extraction and Characterization of Xylan from Sugarcane Tops as a Potential Commercial Substrate","volume":"131","author":"Khaire","year":"2021","journal-title":"J. Biosci. Bioeng."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e70239","DOI":"10.1111\/1750-3841.70239","article-title":"Recent Developments in Extraction, Molecular Characterization, Bioactivity, and Application of Brewers Spent Grain Arabinoxylans","volume":"90","author":"Kaur","year":"2025","journal-title":"J. Food Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Amorim, C., Silv\u00e9rio, S.C., Prather, K.L.J., and Rodrigues, L.R. (2019). From Lignocellulosic Residues to Market: Production and Commercial Potential of Xylooligosaccharides. Biotechnol. Adv., 37.","DOI":"10.1016\/j.biotechadv.2019.05.003"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1541-4337.2010.00135.x","article-title":"Xylooligosaccharides (XOS) as an Emerging Prebiotic: Microbial Synthesis, Utilization, Structural Characterization, Bioactive Properties, and Applications","volume":"10","author":"Aachary","year":"2011","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1046\/j.1529-8817.2003.02047.x","article-title":"Interactions of the Mix-Linked Beta-(1,3)\/-(1,4)-d-Xylans in the Cell Walls of Palmaria almata (Rhodophyta)","volume":"39","author":"Deniaud","year":"2003","journal-title":"J. Phycol."},{"key":"ref_47","unstructured":"Schmedes, P.S. (2020). Investigating Hatchery and Cultivation Methods for Improved Cultivation of Palmaria palmata. [Ph.D. Thesis, DTU aqua, Technical University of Denmark]."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Lopes, D., Melo, T., Meneses, J., Abreu, M.H., Pereira, R., Domingues, P., Lilleb\u00f8, A.I., Calado, R., and Domingues, M.R. (2019). A New Look for the Red Macroalga Palmaria palmata: A Seafood with Polar Lipids Rich in EPA and with Antioxidant Properties. Mar. Drugs, 17.","DOI":"10.3390\/md17090533"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s10811-022-02899-5","article-title":"Concise Review of the Red Macroalga Dulse, Palmaria palmata (L.) Weber & Mohr","volume":"35","author":"Schmedes","year":"2023","journal-title":"J. Appl. Phycol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lafeuille, B., Tamigneaux, \u00c9., Berger, K., Provencher, V., and Beaulieu, L. (2023). Impact of Harvest Month and Drying Process on the Nutritional and Bioactive Properties of Wild Palmaria palmata from Atlantic Canada. Mar. Drugs, 21.","DOI":"10.3390\/md21070392"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"103606","DOI":"10.1016\/j.algal.2024.103606","article-title":"A Critical Review of the Edible Seaweed Palmaria palmata (L.) Weber & Mohr and Its Bioactive Compounds in the \u201cOmics\u201d Era","volume":"82","author":"Xu","year":"2024","journal-title":"Algal Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"103608","DOI":"10.1016\/j.algal.2024.103608","article-title":"Biorefinery of Red Seaweed Palmaria palmata for Production of Bio-Based Chemicals and Biofuels","volume":"82","author":"Dussan","year":"2024","journal-title":"Algal Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s13399-016-0227-5","article-title":"The Nutritional Aspects of Biorefined Saccharina latissima, Ascophyllum nodosum and Palmaria palmata","volume":"7","author":"Schiener","year":"2017","journal-title":"Biomass Convers. Biorefin."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Echave, J., Louren\u00e7o-Lopes, C., Carreira-Casais, A., Chamorro, F., Fraga-Corral, M., Otero, P., Garcia-Perez, P., Baamonde, S., Fern\u00e1ndez-Saa, F., and Cao, H. (2021). Nutritional Composition of the Atlantic Seaweeds Ulva rigida, Codium tomentosum, Palmaria palmata and Porphyra purpurea. Chem. Proc., 5.","DOI":"10.3390\/CSAC2021-10681"},{"key":"ref_55","first-page":"125","article-title":"Seasonal and Geographical Variation in the Chemical Composition of the Red Alga Palmaria palmata (L.) Kuntze","volume":"47","author":"Larsen","year":"2004","journal-title":"Bot. Mar."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Idowu, A.T., Amigo-Benavent, M., Whelan, S., Edwards, M.D., and FitzGerald, R.J. (2023). Impact of Different Light Conditions on the Nitrogen, Protein, Colour, Total Phenolic Content and Amino Acid Profiles of Cultured Palmaria palmata. Foods, 12.","DOI":"10.3390\/foods12213940"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1007\/s10811-022-02731-0","article-title":"Impact of Temperature and Cooking Time on the Physicochemical Properties and Sensory Potential of Seaweed Water Extracts of Palmaria palmata and Saccharina longicruris","volume":"34","author":"Lafeuille","year":"2022","journal-title":"J. Appl. Phycol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s10811-010-9632-5","article-title":"Bioactive Compounds in Seaweed: Functional Food Applications and Legislation","volume":"23","author":"Holdt","year":"2011","journal-title":"J. Appl. Phycol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s10811-020-02295-x","article-title":"Bio-Processing of Macroalgae Palmaria palmata: Metabolite Fractionation from Pressed Fresh Material and Ensiling Considerations for Long-Term Storage","volume":"33","author":"Gallagher","year":"2021","journal-title":"J. Appl. Phycol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1016\/j.lwt.2011.06.008","article-title":"Influence of Hot-Air Temperature on Drying Kinetics, Functional Properties, Colour, Phycobiliproteins, Antioxidant Capacity, Texture and Agar Yield of Alga Gracilaria chilensis","volume":"44","year":"2011","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Silva, A.F.R., Abreu, H., Silva, A.M.S., and Cardoso, S.M. (2019). Effect of Oven-Drying on the Recovery of Valuable Compounds from Ulva rigida, Gracilaria Sp. and Fucus vesiculosus. Mar. Drugs, 17.","DOI":"10.3390\/md17020090"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Ashworth, H., Donohoe, P., and Kibblewhite, B. (2024). Seaweed and the Applicability of Freeze Drying Techniques. Front. Mar. Sci., 11.","DOI":"10.3389\/fmars.2024.1386418"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Le Strat, Y., Mandin, M., Ruiz, N., Robiou du Pont, T., Ragueneau, E., Barnett, A., D\u00e9l\u00e9ris, P., and Dumay, J. (2023). Quantification of Xylanolytic and Cellulolytic Activities of Fungal Strains Isolated from Palmaria palmata to Enhance R-Phycoerythrin Extraction of Palmaria palmata: From Seaweed to Seaweed. Mar. Drugs, 21.","DOI":"10.3390\/md21070393"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.foodchem.2013.10.113","article-title":"Physicochemical Factors Affecting the Stability of Two Pigments: R-Phycoerythrin of Grateloupia turuturu and B-Phycoerythrin of Porphyridium cruentum","volume":"150","author":"Munier","year":"2014","journal-title":"Food Chem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0378-4347(99)00433-8","article-title":"One-Step Purification of R-Phycoerythrin from the Red Macroalga Palmaria palmata Using Preparative Polyacrylamide Gel Electrophoresis","volume":"739","author":"Pons","year":"2000","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Jang, S.-K., Kim, J.-H., Choi, J.-H., Cho, S.-M., Kim, J.-C., Kim, H., and Choi, I.-G. (2021). Evaluation of Xylooligosaccharides Production for a Specific Degree of Polymerization by Liquid Hot Water Treatment of Tropical Hardwood. Foods, 10.","DOI":"10.3390\/foods10020463"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Sun, Q. (2022). The Hydrophobic Effects: Our Current Understanding. Molecules, 27.","DOI":"10.3390\/molecules27207009"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.procbio.2019.03.030","article-title":"Enzymatic Production of Xylooligosaccharides from Red Alga Dulse (Palmaria Sp.) Wasted in Japan","volume":"82","author":"Yamamoto","year":"2019","journal-title":"Process Biochem."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1080\/00318884.2022.2117932","article-title":"Morphological Variation in Northwest Pacific Devaleraea mollis and Description of D. Inkyuleei Sp. Nov. (Palmariaceae, Rhodophyta)","volume":"61","author":"Skriptsova","year":"2022","journal-title":"Phycologia"},{"key":"ref_70","unstructured":"AOAC (2008). AOAC Official Methods of Analysis, The Association of Official Analytical Chemists. [17th ed.]."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A Rapid Method of Total Lipid Extraction and Purifcation","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.3390\/molecules20011118","article-title":"Phenolic Content and Antioxidant Capacity in Algal Food Products","volume":"20","author":"Machu","year":"2015","journal-title":"Molecules"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"100335","DOI":"10.1016\/j.fufo.2024.100335","article-title":"Tuning the Extraction Methodology Targeting Protein-Enriched Fractions from Red Algae","volume":"9","author":"Nunes","year":"2024","journal-title":"Future Foods"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"134700","DOI":"10.1016\/j.foodchem.2022.134700","article-title":"Rapid and Accurate Determination of Protein Content in North Atlantic Seaweed by NIR and FTIR Spectroscopies","volume":"404","author":"Niemi","year":"2023","journal-title":"Food Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1021\/ac60147a030","article-title":"Use of DNS Reagent for Determination of Reducing Sugar","volume":"31","author":"Miller","year":"1959","journal-title":"Anal. Chem."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/8\/302\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:17:45Z","timestamp":1760033865000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/8\/302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,29]]},"references-count":75,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["md23080302"],"URL":"https:\/\/doi.org\/10.3390\/md23080302","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,29]]}}}