{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T04:40:37Z","timestamp":1759207237870,"version":"3.44.0"},"reference-count":62,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:00:00Z","timestamp":1758931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>This study details a biorefinery approach to valorize Dosidicus gigas squid pen waste. The process starts with the enzymatic deproteinization of squid pens, which prove effective with both Alcalase and Novozym, with the latter exhibiting a slightly higher efficiency to yield a material with 73% chitin content. Subsequent alkaline hydrolysis produces highly deacetylated chitosan (&gt;90% degree of deacetylation), followed by controlled depolymerization to obtain polymers with molecular weights ranging from 50 to 251 kDa. Both native and depolymerized chitosan exhibit antimicrobial activity against Escherichia coli and Bacillus cereus, with B. cereus demonstrating greater resistance to chitosan compared to E. coli. The research also explores the bioconversion of deproteinization and deacetylation effluents. Deproteinization effluents prove superior in sustaining microbial growth, supporting comparable growth and lactic acid production for human probiotic strains (Lactobacillus plantarum and Leuconostoc mesenteroides) when substituting commercial peptones. Marine bacteria (Pseudomonas fluorescens and Phaeobacter sp.) show lower productivity. Integrating these processes into a biorefinery framework enables the conversion of 1 kg of dry squid pens into 350 g of chitosan, and facilitates the production of 937\u2013949 g of lactic acid using human lactic acid bacteria cultures in media formulated with squid pen-derived effluents, glucose, yeast extract, and mineral salts. This integrated approach highlights the potential for maximizing resource utilization from squid pen waste, reducing environmental impact and generating high-value bioproducts.<\/jats:p>","DOI":"10.3390\/md23100382","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T12:59:46Z","timestamp":1759150786000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sustainable and Integral Valorization of Dosidicus gigas Pen Waste: Combined Production of Chitosan with Antibacterial Properties and Human and Marine Probiotics"],"prefix":"10.3390","volume":"23","author":[{"given":"Marta","family":"Lima","sequence":"first","affiliation":[{"name":"LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7843-4111","authenticated-orcid":false,"given":"Adri\u00e1n","family":"Pedreira","sequence":"additional","affiliation":[{"name":"Recycling and Valorization of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, Spain"},{"name":"Bioprocess Engineering (Bio2eng), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7591-4382","authenticated-orcid":false,"given":"Noelia","family":"Sanz","sequence":"additional","affiliation":[{"name":"Recycling and Valorization of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1122-4726","authenticated-orcid":false,"given":"Jos\u00e9 Antonio","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Recycling and Valorization of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9538-6388","authenticated-orcid":false,"given":"M\u00edriam R.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Bioprocess Engineering (Bio2eng), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-1037","authenticated-orcid":false,"given":"Filipe","family":"Mergulh\u00e3o","sequence":"additional","affiliation":[{"name":"LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7336-4049","authenticated-orcid":false,"given":"Jesus","family":"Valcarcel","sequence":"additional","affiliation":[{"name":"Recycling and Valorization of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, Spain"},{"name":"Scientific Technical Support Unit (UACT), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,27]]},"reference":[{"key":"ref_1","first-page":"402","article-title":"The biology and ecology of the jumbo squid Dosidicus gigas (Cephalopoda) in Chilean waters: A review","volume":"43","author":"Ulloa","year":"2015","journal-title":"Lat. Am. J. Aquat. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12948","DOI":"10.1073\/pnas.0702043104","article-title":"Invasive range expansion by the Humboldt squid, Dosidicus gigas, in the eastern North Pacific","volume":"104","author":"Zeidberg","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Arkhipkin, A.I., Rodhouse, P.G.K., Pierce, G.J., Sauer, W., Sakai, M., Allcock, L., Arguelles, J., Bower, J.R., Castillo, G., and Ceriola, L. (2015). World Squid Fisheries, Taylor & Francis.","DOI":"10.1080\/23308249.2015.1026226"},{"key":"ref_4","unstructured":"FAO (2024). Fishery and Aquaculture Statistics\u2014Yearbook 2021, FAO. FAO Yearbook of Fishery and Aquaculture Statistics."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"943","DOI":"10.5343\/bms.2016.1103","article-title":"Statolith microstructure, age, and maturity of jumbo squid (Dosidicus gigas) in equatorial waters of the eastern tropical Pacific Ocean","volume":"93","author":"Liu","year":"2017","journal-title":"Bull. Mar. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.fishres.2015.11.001","article-title":"Jumbo squid in the eastern Pacific Ocean: A quarter century of challenges and change","volume":"173","author":"Rodhouse","year":"2016","journal-title":"Fish. Res."},{"key":"ref_7","unstructured":"Zeidberg, L. (2013). Advances in Squid Biology, Ecology and Fisheries. Part I\u2013Myopsid Squids, Nova Science Publishers Inc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/524155a","article-title":"Sustainability: Don\u2019t waste seafood waste","volume":"524","author":"Yan","year":"2015","journal-title":"Nature"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.gee.2018.07.007","article-title":"Shell biorefinery: A comprehensive introduction","volume":"3","year":"2018","journal-title":"Green Energy Environ."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Oyatogun, G.M., Esan, T.A., Akpan, E.I., Adeosun, S.O., Popoola, A.P.I., Imasogie, B.I., Soboyejo, W.O., Afonja, A.A., Ibitoye, S.A., and Abere, V.D. (2020). Chitin, chitosan, marine to market. Handbook of Chitin and Chitosan, Elsevier.","DOI":"10.1016\/B978-0-12-817970-3.00011-0"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Pellis, A., Guebitz, G.M., and Nyanhongo, G.S. (2022). Chitosan: Sources, Processing and Modification Techniques. Gels, 8.","DOI":"10.3390\/gels8070393"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"V\u00e1zquez, J.A., Ramos, P., Mir\u00f3n, J., Valcarcel, J., Sotelo, C.G., and P\u00e9rez-Mart\u00edn, R.I. (2017). Production of chitin from penaeus vannamei by-products to pilot plant scale using a combination of enzymatic and chemical processes and subsequent optimization of the chemical production of chitosan by response surface methodology. Mar. Drugs, 15.","DOI":"10.3390\/md15060180"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.carbpol.2010.04.083","article-title":"A perspective on 30 years research on chitin and chitosan","volume":"84","author":"Domard","year":"2011","journal-title":"Carbohydr. Polym."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.ijbiomac.2017.09.035","article-title":"Chitin and chitosan preparation from shrimp shells Penaeus monodon and its human ovarian cancer cell line, PA-1","volume":"107","author":"Srinivasan","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.jclepro.2018.07.316","article-title":"An integral and sustainable valorisation strategy of squid pen by-products","volume":"201","author":"Ramos","year":"2018","journal-title":"J. Clean. Prod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.carbpol.2017.06.070","article-title":"Optimization of high purity chitin and chitosan production from Illex argentinus pens by a combination of enzymatic and chemical processes","volume":"174","author":"Noriega","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1590\/S0104-14282009000300013","article-title":"A review of the antimicrobial activity of chitosan","volume":"19","author":"Goy","year":"2009","journal-title":"Pol\u00edmeros"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Cheung, R., Ng, T., Wong, J., and Chan, W. (2015). Chitosan: An Update on Potential Biomedical and Pharmaceutical Applications. Mar. Drugs, 13.","DOI":"10.3390\/md13085156"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"D\u2019Almeida, M., Attik, N., Amalric, J., Brunon, C., Renaud, F., Abouelleil, H., Toury, B., and Grosgogeat, B. (2017). Chitosan coating as an antibacterial surface for biomedical applications. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0189537"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.carbpol.2005.10.021","article-title":"Bactericidal and antifungal activities of a low molecular weight chitosan and its N-\/2(3)-(dodec-2-enyl)succinoyl\/-derivatives","volume":"64","author":"Tikhonov","year":"2006","journal-title":"Carbohydr. Polym."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.ijbiomac.2016.01.022","article-title":"Evaluation of different factors affecting antimicrobial properties of chitosan","volume":"85","author":"Hosseinnejad","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.foodchem.2017.01.099","article-title":"Antioxidant and functional properties of protein hydrolysates obtained from squid pen chitosan extraction effluent","volume":"227","author":"Shavandi","year":"2017","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"105738","DOI":"10.1016\/j.jwpe.2024.105738","article-title":"Turning fish canning wastewater into resources: Effluents and operational conditions selection for volatile fatty acids production","volume":"64","author":"Carballa","year":"2024","journal-title":"J. Water Process Eng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s00449-006-0110-z","article-title":"Biotechnological production of lactic acid integrated with fishmeal wastewater treatment by Rhizopus oryzae","volume":"30","author":"Huang","year":"2007","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"137952","DOI":"10.1016\/j.jclepro.2023.137952","article-title":"Microbial bioconversion of chemical waste effluents from marine gelatin isolation: Production of probiotics under circular economy philosophy","volume":"416","author":"Nogueira","year":"2023","journal-title":"J. Clean. Prod."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"V\u00e1zquez, J.A., Pedreira, A., Salmer\u00f3n, I., Wardhani, D.H., and Valcarcel, J. (2023). The Fermentation of a Marine Probiotic Bacterium on Low-Cost Media Formulated with Industrial Fish Gelatin Waterstreams and Collagen Hydrolysates. Processes, 11.","DOI":"10.3390\/pr11082397"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kozma, M., Acharya, B., and Bissessur, R. (2022). Chitin, Chitosan, and Nanochitin: Extraction, Synthesis, and Applications. Polymers, 14.","DOI":"10.3390\/polym14193989"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.ijbiomac.2020.12.005","article-title":"Chitosan: A review of sources and preparation methods","volume":"169","author":"Kou","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1080\/03602550600916209","article-title":"A Study on the Effects of the Degree of Deacetylation of Chitosan Films on Physical and Antibacterial Properties","volume":"46","author":"Chiu","year":"2007","journal-title":"Polym. Plast. Technol. Eng."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/j.1365-2621.2010.02186.x","article-title":"Antibacterial activity of chitosans with different degrees of deacetylation and viscosities","volume":"45","author":"Jung","year":"2010","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1016\/j.carres.2011.05.021","article-title":"Characteristics of deacetylation and depolymerization of \u03b2-chitin from jumbo squid (Dosidicus gigas) pens","volume":"346","author":"Jung","year":"2011","journal-title":"Carbohydr. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.foodchem.2013.11.165","article-title":"Alkali- or acid-induced changes in structure, moisture absorption ability and deacetylating reaction of \u03b2-chitin extracted from jumbo squid (Dosidicus gigas) pens","volume":"152","author":"Jung","year":"2014","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7016","DOI":"10.1039\/D1GC02223G","article-title":"Innovative methodology for marine collagen\u2013chitosan\u2013fucoidan hydrogels production, tailoring rheological properties towards biomedical application","volume":"23","author":"Carvalho","year":"2021","journal-title":"Green Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.eurpolymj.2017.08.017","article-title":"Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering","volume":"95","author":"Reys","year":"2017","journal-title":"Eur. Polym. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1080\/00914037.2022.2029440","article-title":"Synthesis of nanoparticles of the chitosan-poly((\u03b1,\u03b2)-DL-aspartic acid) polyelectrolite complex as hydrophilic drug carrier","volume":"72","author":"Santiago","year":"2023","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Jana, S., and Jana, S. (2019). Antibacterial Activity of Chitosan-Based Systems BT-Functional Chitosan: Drug Delivery and Biomedical Applications, Springer.","DOI":"10.1007\/978-981-15-0263-7"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jhin.2011.05.002","article-title":"Disinfection: Is it time to reconsider Spaulding?","volume":"78","author":"McDonnell","year":"2011","journal-title":"J. Hosp. Infect."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Tamara, F.R., Lin, C., Mi, F.-L., and Ho, Y.-C. (2018). Antibacterial Effects of Chitosan\/Cationic Peptide Nanoparticles. Nanomaterials, 8.","DOI":"10.3390\/nano8020088"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/j.bbagen.2006.12.003","article-title":"Low molecular weight chitosans\u2014Preparation with the aid of pronase, characterization and their bactericidal activity towards Bacillus cereus and Escherichia coli","volume":"1770","author":"Varadaraj","year":"2007","journal-title":"Biochim. Biophys. Acta-Gen. Subj."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ijfoodmicro.2010.09.012","article-title":"Antimicrobial properties of chitosan and mode of action: A state of the art review","volume":"144","author":"Kong","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_41","unstructured":"Jennings, J.A. (2017). 9\u2014Antimicrobial Applications of Chitosan, Woodhead Publishing."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ke, C.-L., Deng, F.-S., Chuang, C.-Y., and Lin, C.-H. (2021). Antimicrobial Actions and Applications of Chitosan. Polymers, 13.","DOI":"10.3390\/polym13060904"},{"key":"ref_43","unstructured":"Blanch, H.W., and Clark, D.S. (1996). Biochemical Engineering, Marcel Dekker, Inc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.bej.2015.12.012","article-title":"Valorisation of effluents obtained from chemical and enzymatic chitin production of Illex argentinus pen by-products as nutrient supplements for various bacterial fermentations","volume":"116","author":"Caprioni","year":"2016","journal-title":"Biochem. Eng. J."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"V\u00e1zquez, J.A., Dur\u00e1n, A., Nogueira, M., Mendu\u00ed\u00f1a, A., Antunes, J., Freitas, A.C., and Gomes, A.M. (2020). Production of Marine Probiotic Bacteria in a Cost-Effective Marine Media Based on Peptones Obtained from Discarded Fish By-Products. Microorganisms, 8.","DOI":"10.3390\/microorganisms8081121"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1111\/j.1365-2672.2005.02702.x","article-title":"Growth of Lactobacillus plantarum in media containing hydrolysates of fish viscera","volume":"99","author":"Horn","year":"2005","journal-title":"J. Appl. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/s002840010174","article-title":"Evaluation of Nitrogenous Substrates Such as Peptones from Fish:A New Method Based on Gompertz Modeling of Microbial Growth","volume":"42","author":"Guerard","year":"2001","journal-title":"Curr. Microbiol."},{"key":"ref_48","unstructured":"AOAC Association of Official Analytical Chemistry (1997). Methods of Analysis, AOAC International. [15th ed.]."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1021\/ac60139a005","article-title":"Chromatography of Amino Acids on Sulfonated Polystyrene Resins. An Improved System","volume":"30","author":"Moore","year":"1958","journal-title":"Anal. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Pissard, A., Gofflot, S., Baeten, V., Lecler, B., Lorrette, B., Morin, J.-F., and Debode, F. (2025). Chitin Assessment in Insect-Based Products from Reference Methods to Near-Infrared Models. Insects, 16.","DOI":"10.3390\/insects16090924"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11483-013-9327-y","article-title":"Extraction and Characterization of \u03b1-Chitin and Chitosan from Six Different Aquatic Invertebrates","volume":"9","author":"Kaya","year":"2014","journal-title":"Food Biophys."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0008-6215(95)00207-A","article-title":"Molecular weight manipulation of chitosan I: Kinetics of depolymerization by nitrous acid","volume":"277","author":"Allan","year":"1995","journal-title":"Carbohydr. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1021\/bm034054n","article-title":"Light scattering studies of the solution properties of chitosans of varying degrees of acetylation","volume":"4","author":"Sorlier","year":"2003","journal-title":"Biomacromolecules"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.carbpol.2005.04.006","article-title":"Optimal routine conditions for the determination of the degree of acetylation of chitosan by 1 H-NMR","volume":"61","author":"Riguera","year":"2005","journal-title":"Carbohydr. Polym."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1021\/bm100447f","article-title":"Dynamics of Chitosan by 1H NMR Relaxation","volume":"11","author":"Riguera","year":"2010","journal-title":"Biomacromolecules"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1021\/ac60111a017","article-title":"Colorimetric method for determination of sugars and related substances","volume":"28","author":"Dubois","year":"1956","journal-title":"Anal. Chem."},{"key":"ref_58","first-page":"87","article-title":"Enzymes of starch degradation and synthesis","volume":"12","author":"Bernfeld","year":"1951","journal-title":"Adv Enzym."},{"key":"ref_59","unstructured":"(2015). Methods for Dilution Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically (Standard No. CLSI M07)."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1080\/08927010802239154","article-title":"Antagonism between Bacillus cereus and Pseudomonas fluorescens in planktonic systems and in biofilms","volume":"24","author":"Pereira","year":"2008","journal-title":"Biofouling"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.chemosphere.2014.03.008","article-title":"Inhibition of selected bacterial growth by three hydrocarbons: Mathematical evaluation of toxicity using a toxicodynamic equation","volume":"112","author":"Rial","year":"2014","journal-title":"Chemosphere"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"V\u00e1zquez, J.A., Dur\u00e1n, A.I., Mendu\u00ed\u00f1a, A., and Nogueira, M. (2020). Biotechnological Valorization of Food Marine Wastes: Microbial Productions on Peptones Obtained from Aquaculture By-Products. Biomolecules, 10.","DOI":"10.3390\/biom10081184"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/10\/382\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T04:20:01Z","timestamp":1759206001000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/10\/382"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,27]]},"references-count":62,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["md23100382"],"URL":"https:\/\/doi.org\/10.3390\/md23100382","relation":{},"ISSN":["1660-3397"],"issn-type":[{"type":"electronic","value":"1660-3397"}],"subject":[],"published":{"date-parts":[[2025,9,27]]}}}