{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T10:35:48Z","timestamp":1772361348882,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T00:00:00Z","timestamp":1320624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by the soft agar overlay technique. The host specificity was evaluated by cross infection. The ecological impact of phage addition on the structure of the bacterial community was evaluated by DGGE of PCR amplified 16S rRNA gene fragments. The survival period varied between 12 and 91 days, with a higher viability for Aeromonas salmonicida phages. The phages of Vibrio parahaemolyticus and of A. salmonicida infected bacteria of different families with a high efficacy of plating. The specific phages of pathogenic bacteria had no detectable impact on the structure of the bacterial community. In conclusion, V. parahaemolyticus and A. salmonicida phages show good survival time in marine water, have only a moderated impact on the overall bacterial community structure and the desired specificity for host pathogenic bacteria, being potential candidates for therapy of fish infectious diseases in marine aquaculture systems.<\/jats:p>","DOI":"10.3390\/md9112236","type":"journal-article","created":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T11:36:28Z","timestamp":1320665788000},"page":"2236-2255","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community Structure"],"prefix":"10.3390","volume":"9","author":[{"given":"Carla","family":"Pereira","sequence":"first","affiliation":[{"name":"CESAM and Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Yolanda J.","family":"Silva","sequence":"additional","affiliation":[{"name":"CESAM and Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Ana L.","family":"Santos","sequence":"additional","affiliation":[{"name":"CESAM and Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"\u00c2ngela","family":"Cunha","sequence":"additional","affiliation":[{"name":"CESAM and Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Newton C. M.","family":"Gomes","sequence":"additional","affiliation":[{"name":"CESAM and Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8422-8664","authenticated-orcid":false,"given":"Adelaide","family":"Almeida","sequence":"additional","affiliation":[{"name":"CESAM and Department of Biology, Campus of Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2011,11,7]]},"reference":[{"key":"ref_1","unstructured":"(2009). The State of World Fisheries and Aquaculture\u20142008, FAO (Fisheries and Aquaculture Department)."},{"key":"ref_2","unstructured":"(1998). The State of World Fisheries and Aquaculture, FAO (Fisheries and Aquaculture Department)."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquaculture.2006.05.013","article-title":"Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand","volume":"258","author":"Flegel","year":"2006","journal-title":"Aquaculture"},{"key":"ref_4","unstructured":"Saksida, S., Constantine, J., Karreman, G.A., Neville, C., Sweeting, R., and Beamish, R (, January August). Evalution of sea lice, Lepeophtheirus salmonis, abundance levels on farmed salmon in British Columbia, Canada. Cairns, Australia."},{"key":"ref_5","unstructured":"Subasinghe, R.P., Bueno, P., Phillips, M.J., Hough, C., McGladdery, S.E., and Arthur, J.R. (2001)."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0044-8486(91)90284-E","article-title":"Pasteurellosis in cultured gilthead seabream, Sparus aurata: First report in Spain","volume":"99","author":"Toranzo","year":"1991","journal-title":"Aquaculture"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1046\/j.1365-2761.1998.00065.x","article-title":"Vibrio spp. isolated from salmonids with shallow skin lesions and reared at low temperature","volume":"21","author":"Helgason","year":"1998","journal-title":"J. Fish Dis"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1046\/j.1365-2672.1997.00190.x","article-title":"Determination of bacteria associated with reared turbot (Scophthalmus maximus) larvae","volume":"82","author":"Blanch","year":"1997","journal-title":"J. Appl. Microbiol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1007\/PL00012152","article-title":"Survival of Vibrio anguillarum in freshwater environments: Adaptation or debilitation?","volume":"6","author":"Eguchi","year":"2000","journal-title":"J. Infect. Chemother"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1365-2761.1992.tb00637.x","article-title":"Development of monoclonal antibodies for the rapid identification of epizootic Vibrio species","volume":"15","author":"Hanna","year":"1991","journal-title":"J. Fish Dis"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0022-0981(98)00206-8","article-title":"Canges in the composition of Vibrio communities in pond water during tiger shrimp (Penaeus monodon) cultivation and in the hepatopancreas of healthy and diseased shrimp","volume":"239","author":"Sung","year":"1999","journal-title":"J. Exp. Mar. Biol. Ecol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0044-8486(94)00353-P","article-title":"Interactions between peritoneal exudate cells (PECs) of gilthead seabream (Sparus aurata) and Pasteurella piscicida. A morphological study","volume":"131","author":"Noya","year":"1995","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Bernoth, E.M. (1997). Furunculosis: Multidisciplinary Fish Disease Research, Academic Press.","DOI":"10.1016\/B978-012093040-1\/50003-8"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"268","DOI":"10.3390\/md7030268","article-title":"Phage therapy and photodynamic therapy: Low environmental impact approaches to inactivate microorganisms in fish farming plants","volume":"7","author":"Almeida","year":"2009","journal-title":"Mar. Drugs"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.1365-2761.2004.00597.x","article-title":"Effectiveness of a divalent vaccine for sole, Solea senegalensis (Kaup), against Vibrio harveyi and Photobacterium damselae subsppiscicida","volume":"28","author":"Arijo","year":"2005","journal-title":"J. Fish Dis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e187:1801","DOI":"10.1371\/journal.pgen.0020187","article-title":"Virulence attributes and hyphal growth of C. neoformans are quantitative traits and the MAT\u03b1 allele enhances filamentation","volume":"2","author":"Lin","year":"2006","journal-title":"PLoS Genet"},{"key":"ref_17","unstructured":"Reed, P.A., and Francis-Floyd, R (1996). Vibrio Infections of Fish, Institute of Food and Agricultural Sciences, University of Florida."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10123-002-0051-6","article-title":"Photobacterium damselae subsp. piscicida: An integrated view of a bacterial fish pathogen","volume":"1","author":"Romalde","year":"2002","journal-title":"Int. Microbiol"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0923-2508(01)01280-3","article-title":"Bacteriophage therapy of infectious diseases in aquaculture","volume":"153","author":"Nakai","year":"2002","journal-title":"Res. Microbiol"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0044-8486(01)00597-X","article-title":"Viral and bacterial diseases of marine fish and shellfish in Japanese hatcheries","volume":"202","author":"Muroga","year":"2001","journal-title":"Aquaculture"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0044-8486(97)00114-2","article-title":"The use of immunostimulation in marine larviculture: Possibilities and challenges","volume":"155","author":"Vadstein","year":"1997","journal-title":"Aquaculture"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"603","DOI":"10.20506\/rst.15.2.943","article-title":"Geographical spread of bacterial and fungal diseases of crustaceans","volume":"15","author":"Alderman","year":"1996","journal-title":"Rev. Sci. Tech. IOE"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0169-409X(01)00159-4","article-title":"Aquaculture pharmaceuticals and biologicals: Current perspectives and future possibilities","volume":"50","author":"Shao","year":"2001","journal-title":"Adv. Drug Deliv. Rev"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1046\/j.1365-2761.2002.00401.x","article-title":"Proliferative kidney disease in Switzerland: Current state of knowledge","volume":"25","author":"Wahli","year":"2002","journal-title":"J. Fish Dis"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2008). Bacteriophage Ecology: Population Growth, Evolution, and Impact of Bacterial Viruses, Cambridge University Press.","DOI":"10.1017\/CBO9780511541483"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1128\/IAI.70.1.204-210.2002","article-title":"Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin-resistant Enterococcus faecium","volume":"70","author":"Biswas","year":"2002","journal-title":"Infect. Immun"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1086\/374001","article-title":"Experimental protection of mice against lethal Staphylococcus aureus infection by novel bacteriophage phi MR11","volume":"187","author":"Matsuzaki","year":"2003","journal-title":"J. Infect. Dis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1128\/AAC.49.3.1220-1221.2005","article-title":"Experimental bacteriophage protection against Staphylococcus aureus abscesses in a rabbit model","volume":"49","author":"Wills","year":"2005","journal-title":"Antimicrob. Agents Chemother"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3354\/dao053033","article-title":"Bacteriophage control of Pseudomonas plecoglossicida infection in ayu Plecoglossus altivelis","volume":"53","author":"Park","year":"2003","journal-title":"Dis. Aquat. Organ"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s10529-007-9346-1","article-title":"Biotechnological challenges of phage therapy","volume":"29","author":"Skurnik","year":"2007","journal-title":"Biotechnol. Lett"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1577\/H06-019.1","article-title":"Using Bacteriophages to prevent furunculosis caused by Aeromonas salmonicida in farmed brook trout","volume":"18","author":"Imbeault","year":"2006","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.aquaculture.2007.04.049","article-title":"Biocontrol of pathogens in shrimp hatcheries using bacteriophages","volume":"268","author":"Karunasagar","year":"2007","journal-title":"Aquaculture"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3354\/dao037033","article-title":"Protective effects of bacteriophage an experimental Lactococcus gavieae infection in yellowtail","volume":"37","author":"Nakai","year":"1999","journal-title":"Dis. Aquat. Organ"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.aquaculture.2005.12.003","article-title":"Isolation of Vibrio harveyi bacteriophage with a potential for biocontrol of luminous vibriosis in hatchery environments","volume":"255","author":"Vinod","year":"2006","journal-title":"Aquaculture"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3167","DOI":"10.1128\/aem.60.9.3167-3174.1994","article-title":"Dynamics and distribution of cyanophages and their effect on marine Synechococcus spp","volume":"60","author":"Suttle","year":"1994","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3721","DOI":"10.1128\/aem.58.11.3721-3729.1992","article-title":"Mechanisms and rates of decay of marine viruses in seawater","volume":"58","author":"Suttle","year":"1992","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"730","DOI":"10.4319\/lo.1995.40.4.0730","article-title":"Dynamics of a lytic virus infecting the photosynthetic marine picoflagellate Micromonas pusilla","volume":"40","author":"Cottrell","year":"1995","journal-title":"Limnol. Oceanogr"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e193:1248","DOI":"10.1371\/journal.pbio.0040273","article-title":"Viruses\u2019 life history: Towards a mechanistic basis of a trade-off between survival and reproduction among phages","volume":"4","author":"Taddei","year":"2006","journal-title":"PLoS Biol"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3354\/meps063253","article-title":"Biogeochemical significance of bacterial biomass in the ocean\u2019s euphotic 17 zone","volume":"63","author":"Cho","year":"1990","journal-title":"Mar. Ecol. Prog. Ser"},{"key":"ref_40","first-page":"475","article-title":"Status and Future Needs in Protozoan Ecology","volume":"25","author":"Reid","year":"1991","journal-title":"Protozoan and Their Role in Marine Processes, NATO ASI Series G: Ecological 20 Sciences"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6730","DOI":"10.1128\/AEM.01399-07","article-title":"Large variabilities in host strain susceptibility and phage host range govern interactions between lytic marine phages and their Flavobacterium hosts","volume":"73","author":"Holmfeldt","year":"2007","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0923-2508(03)00067-6","article-title":"Bacteriophage observations and evolution","volume":"154","author":"Ackermann","year":"2003","journal-title":"Res. Microbiol"},{"key":"ref_43","unstructured":"Clarke, K.R., and Gorley, R.N. (2001). Primer v5: User Manual\/Tutorial, PRIMER-E."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3817","DOI":"10.1128\/AAC.48.10.3817-3822.2004","article-title":"Therapy of experimental pseudomonas infections with a nonreplicating genetically modified phage","volume":"48","author":"Hagens","year":"2004","journal-title":"Antimicrob. Agents Chemother"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1128\/AAC.00635-06","article-title":"Efficacy of bacteriophage therapy against gut-derived sepsis caused by Pseudomonas aeruginosa in mice","volume":"51","author":"Watanabe","year":"2007","journal-title":"Antimicrob. Agents Chemother"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/21119","article-title":"Marine viruses and their biogeochemical and ecological effects","volume":"399","author":"Fuhrman","year":"1999","journal-title":"Nature"},{"key":"ref_47","first-page":"593","article-title":"Bateriophage therapy: An alternative to conventional antibiotics","volume":"51","author":"Mathur","year":"2003","journal-title":"J. Assoc. Physicians India"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.4319\/lo.2000.45.6.1320","article-title":"Elements of a theory for the mechanisms controlling abundance, diversity, and biogeochemical role of lytic bacterial viruses in aquatic systems","volume":"45","author":"Thingstad","year":"2000","journal-title":"Limnol Oceanogr"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5220","DOI":"10.1128\/JB.185.17.5220-5233.2003","article-title":"Complete genome sequence of the broad-hostrange vibriophage KVP40: Comparative genomics of a t4-related Bacteriophage","volume":"17","author":"Miller","year":"2003","journal-title":"J. Bacteriol"},{"key":"ref_50","unstructured":"Pereira, C (2009). Use of Bacteriophages on the Inactivation of Pathogenic Bacteria in Aquaculture System, University of Aveiro."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/BF00015518","article-title":"Effects of bottle size in determinations of primary productivity by phytoplankton","volume":"173","author":"Fogg","year":"1989","journal-title":"Hydrobiologia"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1039\/c0em00434k","article-title":"Evaluating seasonal dynamics of bacterial communities in marine fish aquaculture: A preliminary study before applying phage therapy","volume":"13","author":"Pereira","year":"2011","journal-title":"J. Environ. Monit"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1039\/b712749a","article-title":"Sewage bacteriophage photoinactivation by cationic porphyrins: A study of charge effect","volume":"7","author":"Costa","year":"2008","journal-title":"Photochem. Photobiol. Sci"},{"key":"ref_54","unstructured":"Louvado, A (2010). Isolamento e Caracteriza\u00e7\u00e3o de Bact\u00e9rias Resistentes a Surfactantes, University of Aveiro."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1111\/j.1574-6968.2006.00217.x","article-title":"Isolation and characterization of bacteriophages infecting Salmonella spp","volume":"258","author":"Billington","year":"2006","journal-title":"FEMS Microbiol. Lett"},{"key":"ref_56","unstructured":"Adams, N.A. (1959). Bacteriophages, John Wiley and Sons Inc."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1007\/s00248-001-0020-1","article-title":"Loss of estuarine bacteria by viral infection and predation in microcosm conditions","volume":"42","author":"Almeida","year":"2001","journal-title":"Microb. Ecol"},{"key":"ref_58","unstructured":"Kemp, P.F., Sherr, B.F., Sherr, E.B., and Cole, J.J (1993). Aquatic Microbial Ecology, Handbook of Methods in Lewis Publishers."},{"key":"ref_59","unstructured":"Sambrook, J., and Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press. [3rd ed]."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"5488","DOI":"10.1128\/AEM.66.12.5488-5491.2000","article-title":"Rapid Method for Coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA and rRNA-based microbial community composition","volume":"66","author":"Griffiths","year":"2000","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.femsec.2004.04.003","article-title":"Molecular sequence analysis of prokariotic diversity in the middle and outer sections of the Portuguese estuary Ria de Aveiro","volume":"49","author":"Henriques","year":"2004","journal-title":"FEMS Microbiol. Ecol"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/jb.173.2.697-703.1991","article-title":"16S ribosomal DNA amplification for phylogenetic study","volume":"173","author":"Weisburg","year":"1991","journal-title":"J. Bacteriol"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1128\/jb.178.19.5636-5643.1996","article-title":"Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis","volume":"178","author":"Nubel","year":"1996","journal-title":"J. Bacteriol"},{"key":"ref_64","unstructured":"Sambrook, J., Fritsch, E.F., and Maniatis, T (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"4742","DOI":"10.1128\/AEM.67.10.4742-4751.2001","article-title":"Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel e1ectrophoresis: Plant-dependent enrichment and seasonal shifts revealed","volume":"67","author":"Smalla","year":"2001","journal-title":"Appl. Environ. Microb"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/9\/11\/2236\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:57:56Z","timestamp":1760219876000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/9\/11\/2236"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,7]]},"references-count":65,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2011,11]]}},"alternative-id":["md9112236"],"URL":"https:\/\/doi.org\/10.3390\/md9112236","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,7]]}}}