{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T16:26:59Z","timestamp":1778084819205,"version":"3.51.4"},"reference-count":26,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"publisher","award":["Research Contract No. 18202"],"award-info":[{"award-number":["Research Contract No. 18202"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/Multi\/04349\/2019"],"award-info":[{"award-number":["UID\/Multi\/04349\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00100\/2020"],"award-info":[{"award-number":["UIDB\/00100\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/BIA\/00329\/2019"],"award-info":[{"award-number":["UID\/BIA\/00329\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Membranes"],"abstract":"<jats:p>Some of the current strategies for the development of scaffolding materials capable of inducing tissue regeneration have been based on the use of polymeric biomaterials. Chitosan, in particular, due to its recognized biological activity has been used in a number of biomedical applications. Aiming the development of chitosan-based membranes with improved cell adhesion and growth properties to be used as skin scaffolds allowing functional tissue replacement, different formulations with chitosan of different molecular weight, poly (vinyl alcohol) and gelatin, were evaluated. To meet the goal of getting ready-to-use scaffolds assuring membranes\u2019 required properties and sterilization, preparation methodology included a lyophilization procedure followed by a final gamma irradiation step. Two radiation dose values were tested. Samples were characterized by TGA, FTIR, and SEM techniques. Their hydrophilic properties, in vitro stability, and biocompatibility were also evaluated. Results show that all membranes present a sponge-type inner structure. Chitosan of low molecular weight and the introduction of gelatin are more favorable to cellular growth leading to an improvement on cells\u2019 morphology and cytoskeletal organization, giving a good perspective to the use of these membranes as potential skin scaffolds.<\/jats:p>","DOI":"10.3390\/membranes11080561","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T09:25:52Z","timestamp":1627291552000},"page":"561","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Chitosan\/PVA Based Membranes Processed by Gamma Radiation as Scaffolding Materials for Skin Regeneration"],"prefix":"10.3390","volume":"11","author":[{"given":"Maria Helena","family":"Casimiro","sequence":"first","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares (C2TN), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa, 2695-066 Bobadela, Portugal"}]},{"given":"Andreia","family":"Pereira","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares (C2TN), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa, 2695-066 Bobadela, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1235-0107","authenticated-orcid":false,"given":"Jo\u00e3o P.","family":"Leal","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural (CQE), Instituto Superior T\u00e9cnico (IST), 2695-066 Bobadela, Portugal"},{"name":"Departamento de Engenharia e Ci\u00eancias Nucleares (DECN), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa, 2695-066 Bobadela, Portugal"}]},{"given":"Gabriela","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centro de Ecologia, Evolu\u00e7\u00e3o e Altera\u00e7\u00f5es Ambientais (cE3c), Departamento de Biologia Animal, Faculdade de Ci\u00eancias da Universidade de Lisboa (FCUL), 1749-016 Lisboa, Portugal"}]},{"given":"Lu\u00eds M.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares (C2TN), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa, 2695-066 Bobadela, Portugal"},{"name":"Departamento de Engenharia e Ci\u00eancias Nucleares (DECN), Instituto Superior T\u00e9cnico (IST), Universidade de Lisboa, 2695-066 Bobadela, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"ref_1","unstructured":"Wilkinson, P.F., and Millington, R. 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