{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:09:20Z","timestamp":1760234960824,"version":"build-2065373602"},"reference-count":80,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:00:00Z","timestamp":1624924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Horizon 2020","award":["727934"],"award-info":[{"award-number":["727934"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/ASP-AGR\/31257\/2017","UIDB\/00239\/2020","UIDP\/04035\/2020","UID\/Multi\/04551\/2013","IF\/00376\/2012\/CP0165\/CT0003","PD\/00035\/2013","PD\/BD\/114417\/2016","PD\/BD\/113475\/2015"],"award-info":[{"award-number":["PTDC\/ASP-AGR\/31257\/2017","UIDB\/00239\/2020","UIDP\/04035\/2020","UID\/Multi\/04551\/2013","IF\/00376\/2012\/CP0165\/CT0003","PD\/00035\/2013","PD\/BD\/114417\/2016","PD\/BD\/113475\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["CRA-RED-00053-16"],"award-info":[{"award-number":["CRA-RED-00053-16"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Climate change scenarios pose major threats to many crops worldwide, including coffee. We explored the primary metabolite responses in two Coffea genotypes, C. canephora cv. Conilon Clone 153 and C. arabica cv. Icatu, grown at normal (aCO2) or elevated (eCO2) CO2 concentrations of 380 or 700 ppm, respectively, under well-watered (WW), moderate (MWD), or severe (SWD) water deficit conditions, in order to assess coffee responses to drought and how eCO2 can influence such responses. Primary metabolites were analyzed with a gas chromatography time-of-flight mass spectrometry metabolomics platform (GC-TOF-MS). A total of 48 primary metabolites were identified in both genotypes (23 amino acids and derivatives, 10 organic acids, 11 sugars, and 4 other metabolites), with differences recorded in both genotypes. Increased metabolite levels were observed in CL153 plants under single and combined conditions of aCO2 and drought (MWD and SWD), as opposed to the observed decreased levels under eCO2 in both drought conditions. In contrast, Icatu showed minor differences under MWD, and increased levels (especially amino acids) only under SWD at both CO2 concentration conditions, although with a tendency towards greater increases under eCO2. Altogether, CL153 demonstrated large impact under MWD, and seemed not to benefit from eCO2 in either MWD and SWD, in contrast with Icatu.<\/jats:p>","DOI":"10.3390\/metabo11070427","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T10:52:46Z","timestamp":1624963966000},"page":"427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Primary Metabolite Profile Changes in Coffea spp. Promoted by Single and Combined Exposure to Drought and Elevated CO2 Concentration"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3257-9777","authenticated-orcid":false,"given":"Ana M.","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Plant Metabolomics Laboratory, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), 2780-157 Oeiras, Portugal"}]},{"given":"Tiago","family":"Jorge","sequence":"additional","affiliation":[{"name":"Plant Metabolomics Laboratory, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0159-6091","authenticated-orcid":false,"given":"Sonia","family":"Osorio","sequence":"additional","affiliation":[{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u201cLa Mayora\u201d, Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Universidad de M\u00e1laga\u2014Consejo Superior de Investigaciones Cient\u00edficas (IHSM-UMA-CSIC), 29071 M\u00e1laga, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2545-8777","authenticated-orcid":false,"given":"Delphine M.","family":"Pott","sequence":"additional","affiliation":[{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u201cLa Mayora\u201d, Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Universidad de M\u00e1laga\u2014Consejo Superior de Investigaciones Cient\u00edficas (IHSM-UMA-CSIC), 29071 M\u00e1laga, Spain"}]},{"given":"Fernando C.","family":"Lidon","sequence":"additional","affiliation":[{"name":"GeoBioSciences, GeoTechnologies and GeoEngineering (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Monte de Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9637-8475","authenticated-orcid":false,"given":"F\u00e1bio M.","family":"DaMatta","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Vegetal, Universidade Federal Vi\u00e7osa (UFV), Vi\u00e7osa 36570-090, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9788-4831","authenticated-orcid":false,"given":"Isabel","family":"Marques","sequence":"additional","affiliation":[{"name":"Plant Stress & Biodiversity Lab, Centro de Estudos Florestais (CEF), Instituto Superior Agronomia (ISA), Universidade de Lisboa (ULisboa), Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6071-6460","authenticated-orcid":false,"given":"Ana I.","family":"Ribeiro-Barros","sequence":"additional","affiliation":[{"name":"GeoBioSciences, GeoTechnologies and GeoEngineering (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Monte de Caparica, Portugal"},{"name":"Plant Stress & Biodiversity Lab, Centro de Estudos Florestais (CEF), Instituto Superior Agronomia (ISA), Universidade de Lisboa (ULisboa), Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7639-7214","authenticated-orcid":false,"given":"Jos\u00e9 C.","family":"Ramalho","sequence":"additional","affiliation":[{"name":"GeoBioSciences, GeoTechnologies and GeoEngineering (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Monte de Caparica, Portugal"},{"name":"Plant Stress & Biodiversity Lab, Centro de Estudos Florestais (CEF), Instituto Superior Agronomia (ISA), Universidade de Lisboa (ULisboa), Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6747-1243","authenticated-orcid":false,"given":"Carla","family":"Ant\u00f3nio","sequence":"additional","affiliation":[{"name":"Plant Metabolomics Laboratory, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,29]]},"reference":[{"key":"ref_1","unstructured":"(2015). 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