{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:00:55Z","timestamp":1774922455035,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T00:00:00Z","timestamp":1680652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"Fungi4Health","doi-asserted-by":"publisher","award":["NORTE-01-0247-FEDER-070171"],"award-info":[{"award-number":["NORTE-01-0247-FEDER-070171"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fungi4Health","doi-asserted-by":"publisher","award":["North Regional Operational Program 2014\/2020"],"award-info":[{"award-number":["North Regional Operational Program 2014\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fungi4Health","doi-asserted-by":"publisher","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Regional Development Fund (ERDF) through NORTE 2020","award":["NORTE-01-0247-FEDER-070171"],"award-info":[{"award-number":["NORTE-01-0247-FEDER-070171"]}]},{"name":"European Regional Development Fund (ERDF) through NORTE 2020","award":["North Regional Operational Program 2014\/2020"],"award-info":[{"award-number":["North Regional Operational Program 2014\/2020"]}]},{"name":"European Regional Development Fund (ERDF) through NORTE 2020","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]},{"name":"National Funds by FCT\u2014Portuguese Foundation for Science and Technology","award":["NORTE-01-0247-FEDER-070171"],"award-info":[{"award-number":["NORTE-01-0247-FEDER-070171"]}]},{"name":"National Funds by FCT\u2014Portuguese Foundation for Science and Technology","award":["North Regional Operational Program 2014\/2020"],"award-info":[{"award-number":["North Regional Operational Program 2014\/2020"]}]},{"name":"National Funds by FCT\u2014Portuguese Foundation for Science and Technology","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The use of ultrasound-assisted extraction (UAE) of bioactive compounds has been increasing because it is a good alternative to the conventional extraction methods. UAE was used to maximize total polyphenol content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity, and ferric reducing antioxidant power (FRAP) of the mushroom Inonotus hispidus using response surface methodology (RSM). Firstly, the effect of 40% (v\/v) ethanol and 80% (v\/v) methanol on the TPC, DPPH scavenging capacity, and FRAP was evaluated. The ethanolic extracts showed a significantly higher (p &lt; 0.0001) TPC, DPPH scavenging capacity, and FRAP than the methanolic extracts. The best condition to produce an extract with the higher TPC and antioxidant activity was achieved when using 40% (v\/v) ethanol, a ratio of 75 mL\/g, and an extraction time of 20 min. The chromatographic profile of the extract obtained in the optimized condition revealed that hispidin is the main polyphenol present in the extracts of I. hispidus, representing, together with hispidin-like compounds, the majority of the phenolic compounds (159.56 \u00b5g\/g DW out of 219.01 \u00b5g\/g DW). The model allowed us to optimize the conditions to maximize the extraction of phenolic compounds with antioxidant activity from I. hispidus, demonstrating its potential as a source of antioxidant compounds, with possible industrial, pharmaceutical, and food applications.<\/jats:p>","DOI":"10.3390\/metabo13040524","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T01:10:27Z","timestamp":1680743427000},"page":"524","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Optimization of Phenolic Compounds Extraction and Antioxidant Activity from Inonotus hispidus Using Ultrasound-Assisted Extraction Technology"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3230-4650","authenticated-orcid":false,"given":"Liliana","family":"Machado-Carvalho","sequence":"first","affiliation":[{"name":"Department of Agronomy, School of Agrarian and Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"T\u00e2nia","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Agronomy, School of Agrarian and Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environment and Biological Sciences, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Inov4Agro\u2014Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production, UTAD, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6291-8413","authenticated-orcid":false,"given":"Alfredo","family":"Aires","sequence":"additional","affiliation":[{"name":"Department of Agronomy, School of Agrarian and Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environment and Biological Sciences, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Inov4Agro\u2014Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production, UTAD, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0963-5785","authenticated-orcid":false,"given":"Guilhermina","family":"Marques","sequence":"additional","affiliation":[{"name":"Department of Agronomy, School of Agrarian and Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"CITAB\u2014Centre for the Research and Technology of Agro-Environment and Biological Sciences, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Inov4Agro\u2014Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production, UTAD, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kumar, K., Mehra, R., Guin\u00e9, R.P.F., Lima, M.J., Kumar, N., Kaushik, R., Ahmed, N., Yadav, A.N., and Kumar, H. 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