{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:23:30Z","timestamp":1760149410397,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T00:00:00Z","timestamp":1690934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FUNDA\u00c7\u00c3O PARA A CI\u00caNCIA E TECNOLOGIA and NMT, S. A.","doi-asserted-by":"publisher","award":["PD\/BDE\/114550\/2016","PD\/BDE\/150627\/2020"],"award-info":[{"award-number":["PD\/BDE\/114550\/2016","PD\/BDE\/150627\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>The employment of advanced analytical techniques and instrumentation enables the tracing of volatile organic compounds (VOCs) in vestigial concentrations (ppbv-pptv range) for several emerging applications, such as the research of disease biomarkers in exhaled air, the detection of metabolites in several biological processes, and the detection of pollutants for air quality control. In this scope, the storage of gaseous samples is crucial for preserving the integrity and stability of the collected set of analytes. This study aims to assess the suitability of three commercially available syringes as air containers (AC) that are commonly used for the collection, storage, isolation, and transportation of samples: glass syringes with glass plungers (AC1), and two plastic syringes, one with plastic plungers (AC2), and one with rubbered plungers (AC3). For this purpose, 99 air samples with different times of storage (from 10 min to 24 h) were analyzed using a Gas Chromatography\u2014Ion Mobility Spectrometry device and the degradation of the samples was properly assessed by comparing the changes in the VOCs\u2019 emission profiles. The quality of the method was assured by via the measurement of the blank\u2019s spectra before each experimental run, as well as by the consecutive measurement of the three replicates for each sample. A statistical analysis of the changes in the VOCs\u2019 emission patterns was performed using principal component analysis (PCA). The results, with a total explained variance of 93.61%, indicate that AC3 is the most suitable option for the long-term storage of air samples. Thus, AC3 containers demonstrated a higher capacity to preserve the stability and integrity of the analytes compared to AC1 and AC2. The findings of the short-term effects analysis, up to 1 h, confirm the suitability of all analyzed syringe-based containers for sample-transferring purposes in onsite analysis.<\/jats:p>","DOI":"10.3390\/metabo13080903","type":"journal-article","created":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T11:24:26Z","timestamp":1690975466000},"page":"903","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Suitability of Short- and Long-Term Storage of Volatile Organic Compounds Samples in Syringe-Based Containers: A Comparison Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1856-0298","authenticated-orcid":false,"given":"Paulo Henrique Costa","family":"Santos","sequence":"first","affiliation":[{"name":"Laboratory for Instrumentation, Biomedical Engineering and Radiation Physics (LIBPhys-UNL), Department of Physics, NOVA School of Science and Technology, NOVA University of Lisbon, Campus FCT-NOVA, 2829-516 Caparica, Portugal"},{"name":"NMT, S. A., Edif\u00edcio Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7008-3401","authenticated-orcid":false,"given":"Pedro Catal\u00e3o","family":"Moura","sequence":"additional","affiliation":[{"name":"Laboratory for Instrumentation, Biomedical Engineering and Radiation Physics (LIBPhys-UNL), Department of Physics, NOVA School of Science and Technology, NOVA University of Lisbon, Campus FCT-NOVA, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7913-7047","authenticated-orcid":false,"given":"Valentina","family":"Vassilenko","sequence":"additional","affiliation":[{"name":"Laboratory for Instrumentation, Biomedical Engineering and Radiation Physics (LIBPhys-UNL), Department of Physics, NOVA School of Science and Technology, NOVA University of Lisbon, Campus FCT-NOVA, 2829-516 Caparica, Portugal"},{"name":"NMT, S. A., Edif\u00edcio Madan Parque, Rua dos Inventores, 2825-182 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Orecchio, S., Fiore, M., Barreca, S., and Vara, G. (2017). Volatile profiles of emissions from different activities analyzed using canister samplers and gas chromatography-mass spectrometry (GC\/MS) analysis: A case study. Int. J. Environ. Res. Public Health, 14.","DOI":"10.3390\/ijerph14020195"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Barreca, S., La Bella, S., Maggio, A., Licata, M., Buscemi, S., Leto, C., Pace, A., and Tuttolomondo, T. (2021). Flavoring extra-virgin olive oil with aromatic and medicinal plants essential oils stabilizes oleic acid composition during photo-oxidative stress. Agriculture, 11.","DOI":"10.3390\/agriculture11030266"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0378-4347(99)00127-9","article-title":"Variation in volatile organic compounds in the breath of normal humans","volume":"729","author":"Phillips","year":"1999","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.lungcan.2011.08.009","article-title":"An electronic nose distinguishes exhaled breath of patients with Malignant Pleural Mesothelioma from controls","volume":"75","author":"Dragonieri","year":"2012","journal-title":"Lung Cancer"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1002\/bmc.835","article-title":"Human exhaled air analytics: Biomarkers of diseases","volume":"21","author":"Buszewski","year":"2007","journal-title":"Biomed. Chromatogr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1146\/annurev-anchem-071213-020043","article-title":"Analysis of exhaled breath for disease detection","volume":"7","author":"Amann","year":"2014","journal-title":"Annu. Rev. Anal. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.envpol.2016.05.033","article-title":"Measurements of major VOCs released into the closed cabin environment of different automobiles under various engine and ventilation scenarios","volume":"215","author":"Kim","year":"2016","journal-title":"Environ. Pollut."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.scitotenv.2016.09.009","article-title":"Exposure to pesticides and the associated human health effects","volume":"575","author":"Kim","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.envint.2013.07.019","article-title":"A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects","volume":"60","author":"Kim","year":"2013","journal-title":"Environ. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9018","DOI":"10.1016\/j.atmosenv.2008.09.052","article-title":"Semivolatile organic compounds in indoor environments","volume":"42","author":"Weschler","year":"2008","journal-title":"Atmos. Environ."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Moura, P.C., and Vassilenko, V. (2023). Long-term in situ air quality assessment in closed environments: A gas chromatography\u2013ion mobility spectrometry applicability study. Eur. J. Mass Spectrom.","DOI":"10.1177\/14690667231187502"},{"key":"ref_12","unstructured":"Moura, P.C., Vassilenko, V., Fernandes, J.M., and Santos, P.H. (2020). Technological Innovation for Life Improvement, Proceedings of the 11th IFIP WG 5.5\/SOCOLNET Advanced Doctoral Conference on Computing, Electrical and Industrial Systems, DoCEIS 2020, Caparica, Portugal, 1\u20133 July 2020, Springer."},{"key":"ref_13","unstructured":"Santos, P., Vassilenko, V., Conduto, C., Fernandes, J.M., Moura, P.C., and Bonif\u00e1cio, P. (2021). Technological Innovation for Applied AI Systems, Proceedings of the 12th IFIP WG 5.5\/SOCOLNET Advanced Doctoral Conference on Computing, Electrical and Industrial Systems, DoCEIS 2021, Caparica, Portugal, 7\u20139 July 2021, Springer."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Santos, P.H.C., Vassilenko, V., Moura, P.C., Conduto, C., Fernandes, J.M., and Bonif\u00e1cio, P. (2021, January 20). Instrumentation for differentiation of exhaled air. Proceedings of the Fifteenth International Conference on Correlation Optics, Chernivtsi, Ukraine.","DOI":"10.1117\/12.2617391"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s40825-022-00220-x","article-title":"Ion Mobility Spectrometry Towards Environmental Volatile Organic Compounds Identification and Quantification: A Comparative Overview over Infrared Spectroscopy","volume":"9","author":"Moura","year":"2023","journal-title":"Emiss. Control Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Horvath, I., and Jongste, J.C. (2010). Exhaled Biomarkers, Maney Publishing. [1st ed.].","DOI":"10.1183\/1025448x.erm4910"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"465","DOI":"10.3390\/metabo4020465","article-title":"Breath analysis in disease diagnosis: Methodological considerations and applications","volume":"4","author":"Turner","year":"2014","journal-title":"Metabolites"},{"key":"ref_18","unstructured":"(2023, June 01). Determination of Volatile Organic Compounds (VOCs), Available online: https:\/\/nepis.epa.gov\/Exe\/ZyNET.exe\/P100YDPO.TXT?ZyActionD=ZyDocument&Client=EPA&Index=2016+Thru+2020&Docs=&Query=&Time=&EndTime=&SearchMethod=1&TocRestrict=n&Toc=&TocEntry=&QField=&QFieldYear=&QFieldMonth=&QFieldDay=&IntQFieldOp=0&ExtQFieldOp=0&XmlQuery=&File=D%3A%5Czyfiles%5CIndex%20Data%5C16thru20%5CTxt%5C00000017%5CP100YDPO.txt&User=ANONYMOUS&Password=anonymous&SortMethod=h%7C-&MaximumDocuments=1&FuzzyDegree=0&ImageQuality=r75g8\/r75g8\/x150y150g16\/i425&Display=hpfr&DefSeekPage=x&SearchBack=ZyActionL&Back=ZyActionS&BackDesc=Results%20page&MaximumPages=1&ZyEntry=1&SeekPage=x&ZyPURL."},{"key":"ref_19","unstructured":"Environmental Applications (2023, June 01). Compendium Method TO-15. Storage Stability of 66 Volatile Organic Compounds (VOCs). Available online: https:\/\/www.restek.com\/row\/technical-literature-library\/articles\/storage-stability-of-66-volatile-organic-compounds-VOCs-in-silicon-lined-air-canisters-for-30-days?utm_source=google.com&utm_medium=organic."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1016\/1352-2310(95)00192-2","article-title":"Applicability of canisters for sample storage in the determination of hazardous air pollutants","volume":"29","author":"Kelly","year":"1995","journal-title":"Atmos. Environ."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9842569","DOI":"10.1155\/2020\/9842569","article-title":"Storage Stability of Volatile Organic Compounds from Petrochemical Plant of China in Different Sample Bags","volume":"2020","author":"Han","year":"2020","journal-title":"J. Anal. Methods Chem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Bacaloni, A., Insogna, S., and Zoccolillo, L. (2011). Indoor Air Quality. Volatile Organic Compounds: Sources, Sampling and Analysis, Intechopen. [1st ed.].","DOI":"10.5772\/21645"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"163497","DOI":"10.1016\/j.scitotenv.2023.163497","article-title":"Temperature driven variations in VOC emissions from plastic products and their fate indoors: A chamber experiment and modelling study","volume":"881","author":"Beel","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4209\/aaqr.2003.06.0003","article-title":"Stability of Trace-level Volatile Organic Compounds Stored in Canisters and Tedlar Bags","volume":"3","author":"Hsieh","year":"2003","journal-title":"Aerosol Air Qual. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"117012","DOI":"10.1016\/j.ijms.2023.117012","article-title":"Contemporary ion mobility spectrometry applications and future trends towards environmental, health and food research: A review","volume":"486","author":"Moura","year":"2023","journal-title":"Int. J. Mass Spectrom."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.aca.2011.07.021","article-title":"A review of recent, unconventional applications of ion mobility spectrometry (IMS)","volume":"703","author":"Armenta","year":"2011","journal-title":"Anal. Chim. Acta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1590\/1806-9282.64.09.861","article-title":"Ion mobility spectrometry: The diagnostic tool of third millennium medicine. Ver","volume":"64","author":"Romero","year":"2018","journal-title":"Assoc. Med. Bras."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Eiceman, G., Karpas, Z., and Hill, H.H. (2013). Ion Mobility Spectrometry, CRC Press. [3rd ed.].","DOI":"10.1201\/b16109"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/S0165-9936(02)00813-0","article-title":"The application of gas chromatography to environmental analysis","volume":"21","author":"Santos","year":"2002","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.chroma.2007.10.110","article-title":"Ion mobility spectrometry detection for gas chromatography","volume":"1177","author":"Kanu","year":"2008","journal-title":"J. Chromatogr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.talanta.2018.04.035","article-title":"Ion mobility spectrometer with orthogonal X-Ray source for increased sensitivity","volume":"185","author":"Bunert","year":"2018","journal-title":"Talanta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.cbpa.2017.10.022","article-title":"Fundamentals of ion mobility spectrometry","volume":"42","author":"Gabelica","year":"2018","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1177\/14690667221130170","article-title":"Gas Chromatography\u2014Ion Mobility Spectrometry as a tool for quick detection of hazardous volatile organic compounds in indoor and ambient air: A university campus case study","volume":"28","author":"Moura","year":"2022","journal-title":"Eur. J. Mass Spectrom."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Barreca, S., Forni, C., Colzani, L., Clerici, L., Daverio, D., and Dellavedova, P. (2021). Study on the stability of antibiotics, pesticides, and drugs in water by using a straightforward procedure applying HPLC-mass spectrometric determination for analytical purposes. Separations, 8.","DOI":"10.3390\/separations8100179"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.talanta.2012.10.071","article-title":"Photochemical sample treatment for extracts clean up in PCB analysis from sediments","volume":"103","author":"Barreca","year":"2013","journal-title":"Talanta"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.microc.2016.09.010","article-title":"Platinum and rhodium in wine samples by using voltametric technique","volume":"130","author":"Amorello","year":"2017","journal-title":"Microchem. J."}],"container-title":["Metabolites"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/8\/903\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:24:27Z","timestamp":1760127867000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-1989\/13\/8\/903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,2]]},"references-count":36,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["metabo13080903"],"URL":"https:\/\/doi.org\/10.3390\/metabo13080903","relation":{},"ISSN":["2218-1989"],"issn-type":[{"type":"electronic","value":"2218-1989"}],"subject":[],"published":{"date-parts":[[2023,8,2]]}}}