{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:51:54Z","timestamp":1760986314241,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["075-15-2021-1331"],"award-info":[{"award-number":["075-15-2021-1331"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Metabolites"],"abstract":"<jats:p>Purpose: Immunodeficiency conditions, which are characterized by reduced immune activity that promotes the development of chronic diseases, are needed for efficient monitoring. A promising area of monitoring and early diagnosis of immunodeficiency diseases is the determination of metabolic biomarkers in the blood. Methods: In this work, we identified a set of lipid biomarkers of immunodeficiency states by performing high-performance liquid chromatography\u2013high-resolution mass spectrometry (HPLC-HRMS) analysis of blood plasma samples from mice and processing them with bioinformatics approaches. Potential biomarkers were selected through statistical analysis and further validated by MS\/MS. Conclusions: As a result, 15 lipids were confirmed and selected as potential biomarkers of immunodeficiency states. The selected biomarkers can be further studied and serve as promising targets for the early diagnosis of immunodeficiency diseases.<\/jats:p>","DOI":"10.3390\/metabo15010060","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T10:44:23Z","timestamp":1737024263000},"page":"60","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Study of Lipid Profile for Mice Treated with Cyclophosphamide by HPLC-HRMS and Bioinformatics"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0744-5494","authenticated-orcid":false,"given":"Ekaterina","family":"Demicheva","sequence":"first","affiliation":[{"name":"Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, Russia"},{"name":"Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Ekaterinburg 620049, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5191-3190","authenticated-orcid":false,"given":"Fernando Jonathan","family":"Polanco Espino","sequence":"additional","affiliation":[{"name":"Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9140-451X","authenticated-orcid":false,"given":"Pavel","family":"Vedeneev","sequence":"additional","affiliation":[{"name":"Institute of Chemical Engineering, Ural Federal University, Ekaterinburg 620002, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0369-0786","authenticated-orcid":false,"given":"Vadim","family":"Shevyrin","sequence":"additional","affiliation":[{"name":"Institute of Chemical Engineering, Ural Federal University, Ekaterinburg 620002, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5578-9921","authenticated-orcid":false,"given":"Aleksey","family":"Buhler","sequence":"additional","affiliation":[{"name":"Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5159-4465","authenticated-orcid":false,"given":"Elena","family":"Mukhlynina","sequence":"additional","affiliation":[{"name":"Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, Russia"},{"name":"Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Ekaterinburg 620049, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3479-9730","authenticated-orcid":false,"given":"Olga","family":"Berdiugina","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Ekaterinburg 620049, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1274-5539","authenticated-orcid":false,"given":"Alicia del Carmen","family":"Mondragon","sequence":"additional","affiliation":[{"name":"Laboratorio de Higiene, Inspecci\u00f3n y Control de Alimentos, Departamento de Qu\u00edmica Anal\u00edtica, Nutrici\u00f3n y Bromatolog\u00eda, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9324-1342","authenticated-orcid":false,"given":"Alberto","family":"Cepeda S\u00e1ez","sequence":"additional","affiliation":[{"name":"Laboratorio de Higiene, Inspecci\u00f3n y Control de Alimentos, Departamento de Qu\u00edmica Anal\u00edtica, Nutrici\u00f3n y Bromatolog\u00eda, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2159-1474","authenticated-orcid":false,"given":"Aroa","family":"Lopez-Santamarina","sequence":"additional","affiliation":[{"name":"Laboratorio de Higiene, Inspecci\u00f3n y Control de Alimentos, Departamento de Qu\u00edmica Anal\u00edtica, Nutrici\u00f3n y Bromatolog\u00eda, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1271-513X","authenticated-orcid":false,"given":"Alejandra","family":"Cardelle-Cobas","sequence":"additional","affiliation":[{"name":"Laboratorio de Higiene, Inspecci\u00f3n y Control de Alimentos, Departamento de Qu\u00edmica Anal\u00edtica, Nutrici\u00f3n y Bromatolog\u00eda, Campus Terra, Universidade da Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1702-2065","authenticated-orcid":false,"given":"Olga","family":"Solovyova","sequence":"additional","affiliation":[{"name":"Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg 620075, Russia"},{"name":"Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Ekaterinburg 620049, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6841-1197","authenticated-orcid":false,"given":"Irina","family":"Danilova","sequence":"additional","affiliation":[{"name":"Institute of Natural Sciences 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