{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T03:58:11Z","timestamp":1775102291905,"version":"3.50.1"},"reference-count":125,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>Since the first microfluidic device was developed more than three decades ago, microfluidics is seen as a technology that exhibits unique features to provide a significant change in the way that modern biology is performed. Blood and blood cells are recognized as important biomarkers of many diseases. Taken advantage of microfluidics assets, changes on blood cell physicochemical properties can be used for fast and accurate clinical diagnosis. In this review, an overview of the microfabrication techniques is given, especially for biomedical applications, as well as a synopsis of some design considerations regarding microfluidic devices. The blood cells separation and sorting techniques were also reviewed, highlighting the main achievements and breakthroughs in the last decades.<\/jats:p>","DOI":"10.3390\/mi10090593","type":"journal-article","created":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T10:52:26Z","timestamp":1568112746000},"page":"593","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":137,"title":["Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications"],"prefix":"10.3390","volume":"10","author":[{"given":"Susana O.","family":"Catarino","sequence":"first","affiliation":[{"name":"Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, Campus de Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]},{"given":"Raquel O.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, Campus de Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3884-6496","authenticated-orcid":false,"given":"Diana","family":"Pinho","sequence":"additional","affiliation":[{"name":"Research Centre in Digitalization and Intelligent Robotics (CeDRI), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"CEFT, Faculdade de Engenharia da Universidade do Porto (FEUP), Rua Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1830-0525","authenticated-orcid":false,"given":"Jo\u00e3o M.","family":"Miranda","sequence":"additional","affiliation":[{"name":"CEFT, Faculdade de Engenharia da Universidade do Porto (FEUP), Rua Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2460-0556","authenticated-orcid":false,"given":"Gra\u00e7a","family":"Minas","sequence":"additional","affiliation":[{"name":"Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, Campus de Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3428-637X","authenticated-orcid":false,"given":"Rui","family":"Lima","sequence":"additional","affiliation":[{"name":"CEFT, Faculdade de Engenharia da Universidade do Porto (FEUP), Rua Roberto Frias, 4200-465 Porto, Portugal"},{"name":"MEtRICs, Mechanical Engineering Department, University of Minho, Campus de Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/nature05058","article-title":"The origins and the future of microfluidics","volume":"442","author":"Whitesides","year":"2006","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.mne.2019.01.003","article-title":"30 years of microfluidics","volume":"2","author":"Convery","year":"2019","journal-title":"Micro Nano Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2170\/jjphysiol.51.19","article-title":"Blood flow structure related to red cell flow: A determinant of blood fluidity in narrow microvessels","volume":"51","author":"Mchedlishvili","year":"2001","journal-title":"Jpn. 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