{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:54:05Z","timestamp":1767855245102,"version":"3.49.0"},"reference-count":34,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT funding through the project FIM4Stroke","doi-asserted-by":"publisher","award":["PTDC\/MEC-URG\/29561\/2017"],"award-info":[{"award-number":["PTDC\/MEC-URG\/29561\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>We present a microfluidic chip for protein labeling in the human serum-based matrix. Serum is a complex sample matrix that contains a variety of proteins, and a matrix is used in many clinical tests. In this study, the device performance was tested using commercial serum samples from healthy donors spiked with the following target proteins: cellular fibronectin (c-Fn) and matrix metallopeptidase 9 (MMP9). The microfluidic molds were fabricated using micro milling on acrylic and using stereolithography (SLA) three-dimensional (3D) printing for an alternative method and comparison. A simple quality control was performed for both fabrication mold methods to inspect the channel height of the chip that plays a critical role in the labeling process. The fabricated microfluidic chip shows a good reproducibility and repeatability of the performance for the optimized channel height of 150 \u00b5m. The spiked proteins of c-Fn and MMP9 in the human serum-based matrix, were successfully labeled by the functionalized magnetic nanoparticles (MNPs). The biomarker labeling occurring in the serum was compared using a simple matrix sample: phosphate buffer. The measured signals obtained by using a magnetoresistive (MR) biochip platform showed that the labeling using the proposed microfluidic chip is in good agreement for both matrixes, i.e., the analytical performance (sensitivity) obtained with the serum, near the relevant cutoff values, is within the uncertainty of the measurements obtained with a simple and more controlled matrix: phosphate buffer. This finding is promising for stroke patient stratification where these biomarkers are found at high concentrations in the serum.<\/jats:p>","DOI":"10.3390\/mi13101722","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T22:45:29Z","timestamp":1665614729000},"page":"1722","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Labeling on a Chip of Cellular Fibronectin and Matrix Metallopeptidase-9 in Human Serum"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7543-5143","authenticated-orcid":false,"given":"Briliant Adhi","family":"Prabowo","sequence":"first","affiliation":[{"name":"International Iberian Nanotechnology Laboratory (INL), 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8707-8796","authenticated-orcid":false,"given":"Carole","family":"Sousa","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory (INL), 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6913-6529","authenticated-orcid":false,"given":"Susana","family":"Cardoso","sequence":"additional","affiliation":[{"name":"INESC-MN\u2013 Institute for Systems and Computer Engineering-Microsystems and Nanotechnologies,1000-029 Lisbon, Portugal"}]},{"given":"Paulo","family":"Freitas","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory (INL), 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4558-7047","authenticated-orcid":false,"given":"Elisabete","family":"Fernandes","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory (INL), 4715-330 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.actbio.2016.04.023","article-title":"Ultra-low fouling and high antibody loading zwitterionic hydrogel coatings for sensing and detection in complex media","volume":"40","author":"Chou","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1038\/nprot.2016.003","article-title":"Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics","volume":"11","author":"Warkiani","year":"2016","journal-title":"Nat. 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