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evolved with the appearance of polydimethylsiloxane (PDMS), an elastomer with a short processing time and the possibility for replication on a micrometric scale. Despite the many advantages of PDMS, there are well-known drawbacks, such as the hydrophobic surface, the absorption of small molecules, the low stiffness, relatively high cost, and the difficulty of scaling up the fabrication process for industrial production, creating a need for alternative materials. One option is the use of stiffer thermoplastics, such as the cyclic olefin copolymer (COC), which can be mass produced, have lower cost and possess excellent properties. In this work, a method to fabricate COC microfluidic structures was developed. The work was divided into process optimization and evaluation of material properties for application in microfluidics. In the processing step, moulding, sealing, and liquid handling aspects were developed and optimized. The resulting COC devices were evaluated from the point of view of molecular diffusion, burst pressure, temperature resistance, and susceptibility to surface treatments and these results were compared to PDMS devices. Lastly, a target DNA hybridization assay was performed showing the potential of the COC-based microfluidic device to be used in biosensing and Lab-on-a-Chip applications.<\/jats:p>","DOI":"10.3390\/mi14101837","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T03:04:18Z","timestamp":1695783858000},"page":"1837","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Scalable Processing of Cyclic Olefin Copolymer (COC) Microfluidic Biochips"],"prefix":"10.3390","volume":"14","author":[{"given":"Rodolfo G.","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2014Microsistemas e Nanotecnologias (INESC MN), Rua Alves Redol 9, 1000-029 Lisbon, Portugal"}]},{"given":"Pedro G. M.","family":"Condelipes","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2014Microsistemas e Nanotecnologias (INESC MN), Rua Alves Redol 9, 1000-029 Lisbon, Portugal"}]},{"given":"Rafaela R.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2014Microsistemas e Nanotecnologias (INESC MN), Rua Alves Redol 9, 1000-029 Lisbon, Portugal"}]},{"given":"Virginia","family":"Chu","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2014Microsistemas e Nanotecnologias (INESC MN), Rua Alves Redol 9, 1000-029 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5677-3024","authenticated-orcid":false,"given":"Jo\u00e3o Pedro","family":"Conde","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2014Microsistemas e Nanotecnologias (INESC MN), Rua Alves Redol 9, 1000-029 Lisbon, Portugal"},{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,27]]},"reference":[{"key":"ref_1","unstructured":"Rodrigues, R.G. (2021). New Materials for Microfluidic Biochips\u2014Thermoplastic COC. [Master\u2019s Thesis, Instituto Superior T\u00e9cnico]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.mne.2019.01.003","article-title":"30 Years of Microfluidics","volume":"2","author":"Convery","year":"2019","journal-title":"Micro Nano Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1021\/cr300337x","article-title":"Advances in Microfluidic Materials, Functions, Integration, and Applications","volume":"113","author":"Nge","year":"2013","journal-title":"Chem. Rev."},{"key":"ref_4","unstructured":"Reedijk, J., and Barnett, N. (2014). Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, Elsevier."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jsamd.2020.07.008","article-title":"Advances in Numerical Approaches for Microfluidic Cell Analysis Platforms","volume":"5","author":"Sheidaei","year":"2020","journal-title":"J. Sci. Adv. Mater. Devices"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ortseifen, V., Viefhues, M., Wobbe, L., and Gr\u00fcnberger, A. (2020). Microfluidics for Biotechnology: Bridging Gaps to Foster Microfluidic Applications. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.589074"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"16513","DOI":"10.1038\/s41598-020-73535-w","article-title":"A Novel, Low Cost, and Accessible Method for Rapid Fabrication of the Modifiable Microfluidic Devices","volume":"10","author":"Annabestani","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2656","DOI":"10.1039\/c1lc20125e","article-title":"Disposable Microfluidic Substrates: Transitioning from the Research Laboratory into the Clinic","volume":"11","author":"Kuo","year":"2011","journal-title":"Lab Chip"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"R15","DOI":"10.1088\/0960-1317\/17\/5\/R01","article-title":"Lab-on-Chip Technologies: Making a Microfluidic Network and Coupling It into a Complete Microsystem\u2014A Review","volume":"17","author":"Abgrall","year":"2007","journal-title":"J. Micromech. Microeng."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Iliescu, C., Taylor, H., Avram, M., Miao, J., and Franssila, S. (2012). A Practical Guide for the Fabrication of Microfluidic Devices Using Glass and Silicon. Biomicrofluidics, 6.","DOI":"10.1063\/1.3689939"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2396","DOI":"10.1021\/ar300314s","article-title":"Materials for Microfluidic Chip Fabrication","volume":"46","author":"Ren","year":"2013","journal-title":"Acc. Chem. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3872","DOI":"10.1039\/C8LC01109E","article-title":"Disposable Silicon-Glass Microfluidic Devices: Precise, Robust and Cheap","volume":"18","author":"Qi","year":"2018","journal-title":"Lab Chip"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Tsao, C.-W. (2016). Polymer Microfluidics: Simple, Low-Cost Fabrication Process Bridging Academic Lab Research to Commercialized Production. Micromachines, 7.","DOI":"10.3390\/mi7120225"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.aca.2020.09.013","article-title":"A Practical Guide to Rapid-Prototyping of PDMS-Based Microfluidic Devices: A Tutorial","volume":"1135","author":"Morbioli","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4974","DOI":"10.1021\/ac980656z","article-title":"Rapid Prototyping of Microfluidic Systems in Poly(Dimethylsiloxane)","volume":"70","author":"Duffy","year":"1998","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3938","DOI":"10.1021\/ac300771z","article-title":"Comparison of Biocompatibility and Adsorption Properties of Different Plastics for Advanced Microfluidic Cell and Tissue Culture Models","volume":"84","author":"Janse","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Paoli, R., Di Giuseppe, D., Badiola-Mateos, M., Martinelli, E., Lopez-Martinez, M.J., and Samitier, J. (2021). Rapid Manufacturing of Multilayered Microfluidic Devices for Organ on a Chip Applications. Sensors, 21.","DOI":"10.3390\/s21041382"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10404-009-0421-x","article-title":"Micro-Injection Moulding of Polymer Microfluidic Devices","volume":"7","author":"Attia","year":"2009","journal-title":"Microfluid. Nanofluidics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1109\/JPROC.2003.820548","article-title":"Disposable Smart Lab on a Chip for Point-of-Care Clinical Diagnostics","volume":"92","author":"Ahn","year":"2004","journal-title":"Proc. IEEE"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4409","DOI":"10.1039\/c3lc50888a","article-title":"New Non-Covalent Strategies for Stable Surface Treatment of Thermoplastic Chips","volume":"13","author":"Champ","year":"2013","journal-title":"Lab Chip"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.snb.2017.07.138","article-title":"Rapid Prototyping of Whole-Thermoplastic Microfluidics with Built-in Microvalves Using Laser Ablation and Thermal Fusion Bonding","volume":"255","author":"Shaegh","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Bruijns, B., Veciana, A., Tiggelaar, R., and Gardeniers, H. (2019). Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications. Biosensors, 9.","DOI":"10.3390\/bios9030085"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.snb.2017.03.023","article-title":"Rapid Prototyping of Cyclic Olefin Copolymer (COC) Microfluidic Devices","volume":"247","author":"Aghvami","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.snb.2010.08.043","article-title":"Surface Analysis, Hydrophilic Enhancement, Ageing Behavior and Flow in Plasma Modified Cyclic Olefin Copolymer (COC)-Based Microfluidic Devices","volume":"150","author":"Roy","year":"2010","journal-title":"Sens. Actuators B Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1088\/0960-1317\/17\/2\/020","article-title":"Rapid Prototyping of Microfluidic Chips in COC","volume":"17","author":"Steigert","year":"2007","journal-title":"J. Micromech. Microeng."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1021\/ac001533t","article-title":"A Polymeric Microfluidic Chip for CE\/MS Determination of Small Molecules","volume":"73","author":"Kameoka","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1002\/elps.200410153","article-title":"The Zeta Potential of Cyclo-Olefin Polymer Microchannels and Its Effects on Insulative (Electrodeless) Dielectrophoresis Particle Trapping Devices","volume":"26","author":"Mela","year":"2005","journal-title":"Electrophoresis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00542-011-1366-z","article-title":"Micro Fabrication of Cyclic Olefin Copolymer (COC) Based Microfluidic Devices","volume":"18","author":"Jena","year":"2012","journal-title":"Microsyst. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.colsurfa.2010.04.040","article-title":"Low-Bond Axisymmetric Drop Shape Analysis for Surface Tension and Contact Angle Measurements of Sessile Drops","volume":"364","author":"Stalder","year":"2010","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.colsurfa.2006.03.008","article-title":"A Snake-Based Approach to Accurate Determination of Both Contact Points and Contact Angles","volume":"286","author":"Stalder","year":"2006","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"55008","DOI":"10.1088\/0960-1317\/19\/5\/055008","article-title":"Hot Embossing of Cyclic Olefin Copolymers","volume":"19","author":"Leech","year":"2009","journal-title":"J. Micromech. Microeng."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"100042","DOI":"10.1016\/j.mne.2019.100042","article-title":"Gradient-Temperature Hot-Embossing for Dense Micropillar Array Fabrication on Thick Cyclo-Olefin Polymeric Plates: An Example of a Microfluidic Chromatography Column Fabrication","volume":"5","author":"Kourmpetis","year":"2019","journal-title":"Micro Nano Eng."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5063","DOI":"10.1007\/s00542-017-3282-3","article-title":"Rapid Prototyping of Cyclic Olefin Copolymer Based Microfluidic System with CO2 Laser Ablation","volume":"23","author":"Cai","year":"2017","journal-title":"Microsyst. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1039\/b618901f","article-title":"Low Temperature Bonding of PMMA and COC Microfluidic Substrates Using UV\/Ozone Surface Treatment","volume":"7","author":"Tsao","year":"2007","journal-title":"Lab Chip"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/s10404-016-1823-1","article-title":"Safe and Cost-Effective Rapid-Prototyping of Multilayer PMMA Microfluidic Devices","volume":"20","author":"Liga","year":"2016","journal-title":"Microfluid. Nanofluidics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/j.vacuum.2011.03.007","article-title":"Influence of Plasma Surface Treatment on Thermal Bonding and Flow Behavior in Cyclic Olefin Copolymer (COC) Based Microfluidic Devices","volume":"85","author":"Roy","year":"2011","journal-title":"Vacuum"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bor\u00f3k, A., Laboda, K., and Bony\u00e1r, A. (2021). PDMS Bonding Technologies for Microfluidic Applications: A Review. Biosensors, 11.","DOI":"10.3390\/bios11080292"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1039\/C5LC01498K","article-title":"Tacky Cyclic Olefin Copolymer: A Biocompatible Bonding Technique for the Fabrication of Microfluidic Channels in COC","volume":"16","author":"Keller","year":"2016","journal-title":"Lab Chip"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s10856-009-3696-2","article-title":"Problem of Hydroxyapatite Dispersion in Polymer Matrices: A Review","volume":"20","year":"2009","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1039\/C8LC00458G","article-title":"Capillary Microfluidics in Microchannels: From Microfluidic Networks to Capillaric Circuits","volume":"18","author":"Olanrewaju","year":"2018","journal-title":"Lab Chip"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.mee.2014.10.013","article-title":"Lab-on-a-Chip Devices: How to Close and Plug the Lab?","volume":"132","author":"Temiz","year":"2015","journal-title":"Microelectron. Eng."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5097","DOI":"10.1021\/ac070220w","article-title":"Room-Temperature Bonding for Plastic High-Pressure Microfluidic Chips","volume":"79","author":"Mair","year":"2007","journal-title":"Anal. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1088\/0960-1317\/17\/10\/018","article-title":"Micro Air Bubble Formation and Its Control during Polymerase Chain Reaction (PCR) in Polydimethylsiloxane (PDMS) Microreactors","volume":"17","author":"Liu","year":"2007","journal-title":"J. Micromech. Microeng."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1039\/c0an01034k","article-title":"Bubble-Free on-Chip Continuous-Flow Polymerase Chain Reaction: Concept and Application","volume":"136","author":"Wu","year":"2011","journal-title":"Analyst"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.bios.2018.10.005","article-title":"Bubble-Free Rapid Microfluidic PCR","volume":"126","author":"Lee","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/s10965-007-9119-5","article-title":"Preparation and Characterization of Cyclo Olefin Copolymer (COC)\/Silica Nanoparticle Composites by Solution Blending","volume":"14","author":"Ou","year":"2007","journal-title":"J. Polym. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1039\/c4lc00320a","article-title":"Print Your Own Membrane: Direct Rapid Prototyping of Polydimethylsiloxane","volume":"14","author":"Femmer","year":"2014","journal-title":"Lab Chip"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"S\u00f8nstevold, L., Czerkies, M., Escobedo-Cousin, E., Blonski, S., and Vereshchagina, E. (2023). Application of Polymethylpentene, an Oxygen Permeable Thermoplastic, for Long-Term on-a-Chip Cell Culture and Organ-on-a-Chip Devices. Micromachines, 14.","DOI":"10.3390\/mi14030532"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.snb.2019.01.133","article-title":"Development of a Rapid Bead-Based Microfluidic Platform for DNA Hybridization Using Single- and Multi-Mode Interactions for Probe Immobilization","volume":"286","author":"Caneira","year":"2019","journal-title":"Sens. Actuators B Chem."}],"container-title":["Micromachines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-666X\/14\/10\/1837\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:59:12Z","timestamp":1760129952000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-666X\/14\/10\/1837"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,27]]},"references-count":49,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["mi14101837"],"URL":"https:\/\/doi.org\/10.3390\/mi14101837","relation":{},"ISSN":["2072-666X"],"issn-type":[{"value":"2072-666X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,27]]}}}