{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:21:23Z","timestamp":1760242883463,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:00:00Z","timestamp":1475798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002924","name":"FEDER","doi-asserted-by":"publisher","award":["COMPETE 2020"],"award-info":[{"award-number":["COMPETE 2020"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/CTM\/50025\/2013","PTDC\/SAU-BEB\/102247\/2008"],"award-info":[{"award-number":["UID\/CTM\/50025\/2013","PTDC\/SAU-BEB\/102247\/2008"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"ERDF","doi-asserted-by":"publisher","award":["NORTE-07-0124-FEDER-000058"],"award-info":[{"award-number":["NORTE-07-0124-FEDER-000058"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NORTE 2020","award":["NORTE-01-0145-FEDER-000016"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Micromachines"],"abstract":"<jats:p>This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom  SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting.<\/jats:p>","DOI":"10.3390\/mi7100181","type":"journal-article","created":{"date-parts":[[2016,10,10]],"date-time":"2016-10-10T10:35:19Z","timestamp":1476095719000},"page":"181","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hybrid Microfluidic Platform for Multifactorial Analysis Based on Electrical Impedance, Refractometry, Optical Absorption and Fluorescence"],"prefix":"10.3390","volume":"7","author":[{"given":"F\u00e1bio","family":"Pereira","sequence":"first","affiliation":[{"name":"CENIMAT\/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP\/UNINOVA, 2829-516 Caparica, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Antonio Xavier, Universidade Nova de Lisboa, Avenida da Republica, 2780-157 Oeiras, Portugal"}]},{"given":"Iwona","family":"Bernacka-Wojcik","sequence":"additional","affiliation":[{"name":"CENIMAT\/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP\/UNINOVA, 2829-516 Caparica, Portugal"}]},{"given":"Rita","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"},{"name":"Centre for Applied Photonics, Institute for Systems and Computer Engineering Technology and Science (INESC TEC), Rua do Campo Alegre, 687, 4150-179 Porto, Portugal"}]},{"given":"Maria","family":"Lobato","sequence":"additional","affiliation":[{"name":"CENIMAT\/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP\/UNINOVA, 2829-516 Caparica, Portugal"}]},{"given":"Elvira","family":"Fortunato","sequence":"additional","affiliation":[{"name":"CENIMAT\/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP\/UNINOVA, 2829-516 Caparica, Portugal"}]},{"given":"Rodrigo","family":"Martins","sequence":"additional","affiliation":[{"name":"CENIMAT\/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP\/UNINOVA, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6608-3422","authenticated-orcid":false,"given":"Rui","family":"Igreja","sequence":"additional","affiliation":[{"name":"CENIMAT\/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP\/UNINOVA, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1484-2068","authenticated-orcid":false,"given":"Pedro","family":"Jorge","sequence":"additional","affiliation":[{"name":"Centre for Applied Photonics, Institute for Systems and Computer Engineering Technology and Science (INESC TEC), Rua do Campo Alegre, 687, 4150-179 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7350-649X","authenticated-orcid":false,"given":"Hugo","family":"\u00c1guas","sequence":"additional","affiliation":[{"name":"CENIMAT\/I3N, Department of Materials Science, Faculty of Science and Technology, New University of Lisbon and CEMOP\/UNINOVA, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8320-0937","authenticated-orcid":false,"given":"Abel","family":"Oliva","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Antonio Xavier, Universidade Nova de Lisboa, Avenida da Republica, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2016,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"R15","DOI":"10.1088\/0960-1317\/17\/5\/R01","article-title":"Lab-on-chip technologies: Making a microfluidic network and coupling it into a complete microsystem\u2014A review","volume":"17","author":"Abgrall","year":"2007","journal-title":"J. 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