{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:02:44Z","timestamp":1773288164450,"version":"3.50.1"},"reference-count":95,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology Research"],"abstract":"<jats:p>The Pseudomonas aeruginosa is one of the bacteria that cause serious infections due to resistance to many antibiotics can be fatal in severe cases. Antimicrobial resistance is a global public health concern. To solve this problem, interest in phage therapy has revived; some studies are being developed to try to prove the effectiveness of this therapy. Thus, in this opinion article, several historical aspects are addressed as well some applications of phage therapy against P. aeruginosa.<\/jats:p>","DOI":"10.3390\/microbiolres13010002","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T06:55:03Z","timestamp":1640674503000},"page":"14-37","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The History and Applications of Phage Therapy in Pseudomonas aeruginosa"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1711-0858","authenticated-orcid":false,"given":"Carina","family":"Silva","sequence":"first","affiliation":[{"name":"Laboratory of Medical & Industrial Biotechnology (LABMI), Porto Research, Technology & Innovation Center (PORTIC), R. Arquitecto Lob\u00e3o Vital 172, 4200-374 Porto, Portugal"},{"name":"School of Health (ESS), Polytechnic Institute of Porto (IPP), R. Dr. Ant\u00f3nio Bernardino de Almeida 400, 4200-072 Porto, Portugal"},{"name":"Hospital Santa Maria Maior, Campo da Rep\u00fablica 59, 4750-333 Barcelos, Portugal"},{"name":"Faculty of Biology, University of Vigo, Lagoas-Marcosende, s\/n, 36310 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1376-2624","authenticated-orcid":false,"given":"Sara","family":"S\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Medical & Industrial Biotechnology (LABMI), Porto Research, Technology & Innovation Center (PORTIC), R. Arquitecto Lob\u00e3o Vital 172, 4200-374 Porto, Portugal"},{"name":"School of Medicine, University of Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3277-7821","authenticated-orcid":false,"given":"Carla","family":"Guedes","sequence":"additional","affiliation":[{"name":"Laboratory of Medical & Industrial Biotechnology (LABMI), Porto Research, Technology & Innovation Center (PORTIC), R. Arquitecto Lob\u00e3o Vital 172, 4200-374 Porto, Portugal"},{"name":"Faculty of Biology, University of Vigo, Lagoas-Marcosende, s\/n, 36310 Vigo, Spain"}]},{"given":"Carla","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Medical & Industrial Biotechnology (LABMI), Porto Research, Technology & Innovation Center (PORTIC), R. Arquitecto Lob\u00e3o Vital 172, 4200-374 Porto, Portugal"},{"name":"School of Health (ESS), Polytechnic Institute of Porto (IPP), R. Dr. Ant\u00f3nio Bernardino de Almeida 400, 4200-072 Porto, Portugal"},{"name":"School of Medicine and Biomedical Sciences, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"given":"Cl\u00e1udio","family":"Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Medical & Industrial Biotechnology (LABMI), Porto Research, Technology & Innovation Center (PORTIC), R. 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Antibiotics, 8.","DOI":"10.3390\/antibiotics8030138"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Divya Ganeshan, S., and Hosseinidoust, Z. (2019). Phage Therapy with a Focus on the Human Microbiota. Antibiotics, 8.","DOI":"10.3390\/antibiotics8030131"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.envint.2019.05.062","article-title":"A review of bacteriophage therapy for pathogenic bacteria inactivation in the soil environment","volume":"129","author":"Ye","year":"2019","journal-title":"Environ. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.ijantimicag.2018.09.004","article-title":"Bacteriophage therapy as an alternative treatment for human infections. A comprehensive review","volume":"53","author":"Kakasis","year":"2019","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"7449","DOI":"10.1128\/JVI.00385-15","article-title":"Phage Therapy: A Step Forward in the Treatment of Pseudomonas aeruginosa Infections","volume":"89","author":"Pires","year":"2015","journal-title":"J. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1093\/femsre\/fuaa017","article-title":"Current challenges and future opportunities of phage therapy","volume":"44","author":"Pires","year":"2020","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_7","unstructured":"Marques, D.R.A. (2015). Preval\u00eancia e Susceptibilidade de Isolados Cl\u00ednicos de Pseudomonas aeruginosa numa unidade hospitalar de Portugal. [Ph.D. Thesis, University of Minho]."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.jhin.2009.04.020","article-title":"Pseudomonas aeruginosa: A formidable and ever-present adversary","volume":"73","author":"Kerr","year":"2009","journal-title":"J. Hosp. Infect."},{"key":"ref_9","first-page":"17","article-title":"Los bacteri\u00f3fagos como una alternativa en el tratamiento de enfermedades infecciosas Bacterianas (Fagoterapia)","volume":"41","author":"Hernandez","year":"2010","journal-title":"Rev. Mex. Ciencias Farm."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.biotechadv.2018.11.013","article-title":"Antibiotic resistance in Pseudomonas aeruginosa: Mechanisms and alternative therapeutic strategies","volume":"37","author":"Pang","year":"2019","journal-title":"Biotechnol. Adv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1111\/jam.12463","article-title":"Virulence factors and infection ability of Pseudomonas aeruginosa isolates from a hydropathic facility and respiratory infections","volume":"116","author":"Pereira","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_12","first-page":"77","article-title":"Use of antibiotics and bacterial resistances: Brief notes on its evolution","volume":"34","author":"Loureiro","year":"2016","journal-title":"Rev. Port. Sa\u00fade Publica"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.gendis.2019.04.001","article-title":"Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review","volume":"6","author":"Pachori","year":"2019","journal-title":"Genes Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3389\/fcimb.2017.00039","article-title":"Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence","volume":"7","author":"Moradali","year":"2017","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1097\/MRM.0b013e3283587727","article-title":"\u03b2-lactams: Chemical structure, mode of action and mechanisms of resistance","volume":"24","author":"Fernandes","year":"2013","journal-title":"Rev. Med. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3389\/fcimb.2019.00022","article-title":"Fighting Pathogenic Bacteria on Two Fronts: Phages and Antibiotics as Combined Strategy","volume":"9","author":"Tagliaferri","year":"2019","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"226","DOI":"10.4161\/viru.25991","article-title":"A historical overview of bacteriophage therapy as an alternative to antibiotics for the treatment of bacterial pathogens","volume":"5","author":"Wittebole","year":"2014","journal-title":"Virulence"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1128\/AAC.45.3.649-659.2001","article-title":"Bacteriophage therapy","volume":"45","author":"Sulakvelidze","year":"2001","journal-title":"Antimicrob Agents Chemother."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1743-422X-9-9","article-title":"Bacteriophages and their implications on future biotechnology: A review","volume":"9","author":"Haq","year":"2012","journal-title":"Virol. J."},{"key":"ref_20","first-page":"485","article-title":"Phage therapy","volume":"1915","author":"Green","year":"2019","journal-title":"Encycl. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/S0140-6736(01)20383-3","article-title":"An investigation on the nature of ultra-microscopic viruses","volume":"186","author":"Twort","year":"1915","journal-title":"Lancet."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Lawrence, D., Baldridge, M.T., and Handley, S.A. (2019). Phages and Human Health: More Than Idle Hitchhikers. Viruses, 11.","DOI":"10.3390\/v11070587"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1155\/2007\/976850","article-title":"Phage as an antimicrobial agent: d\u2019Herelle\u2019s heretical theories and their role in the decline of phage prophylaxis in the West","volume":"18","author":"Fruciano","year":"2007","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_24","unstructured":"Fourth, E. (2019). Bacteriophage: Overview. Schmidt TMBT-E of M, Oxford Academic Press."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Nikolich, M.P., and Filippov, A.A. (2020). Bacteriophage Therapy: Developments and Directions. Antibiotics, 9.","DOI":"10.3390\/antibiotics9030135"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"McCallin, S., Sacher, J.C., Zheng, J., and Chan, B.K. (2019). Current State of Compassionate Phage Therapy. Viruses, 11.","DOI":"10.3390\/v11040343"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/bies.201400152","article-title":"A century of phage research: Bacteriophages and the shaping of modern biology","volume":"37","author":"Keen","year":"2015","journal-title":"Bioessays"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Savva, E.-R. (2020). Bacteriophages: Their Structural Organisation and Function. Bacteriophages-Perspectives and Future, IntechOpen.","DOI":"10.5772\/intechopen.73439"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Adriaenssens, E.M., and Rodney Brister, J. (2017). How to name and classify your phage: An informal guide. Viruses, 9.","DOI":"10.1101\/111526"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Chibani, C.M., Farr, A., Klama, S., Dietrich, S., and Liesegang, H. (2019). Classifying the Unclassified: A Phage Classification Method. Viruses, 11.","DOI":"10.3390\/v11020195"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"806","DOI":"10.3390\/v5030806","article-title":"Understanding bacteriophage specificity in natural microbial communities","volume":"5","author":"Koskella","year":"2013","journal-title":"Viruses"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"145","DOI":"10.33073\/pjm-2010-023","article-title":"Bacteriophage Receptors, Mechanisms of Phage Adsorption and Penetration into Host Cell","volume":"59","author":"Rakhuba","year":"2010","journal-title":"Pol. J. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.bpj.2010.04.048","article-title":"Is the In Vitro Ejection of Bacteriophage DNA Quasistatic? A Bulk to Single Virus Study","volume":"99","author":"Chiaruttini","year":"2010","journal-title":"Biophys J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1155\/2006\/329465","article-title":"Phage Therapy-Everything Old is New Again","volume":"17","author":"Kropinski","year":"2006","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nrmicro.2017.61","article-title":"Pseudomonas predators: Understanding and exploiting phage-host interactions","volume":"15","author":"Hendrix","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"12655","DOI":"10.1021\/acs.chemrev.6b00114","article-title":"Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses","volume":"116","author":"Weigele","year":"2016","journal-title":"Chem. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nature09523","article-title":"The CRISPR\/cas bacterial immune system cleaves bacteriophage and plasmid DNA","volume":"468","author":"Garneau","year":"2010","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1038\/nature11723","article-title":"Bacteriophage genes that inactivate the CRISPR\/Cas bacterial immune system","volume":"493","author":"Pawluk","year":"2013","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1080\/15476286.2019.1582974","article-title":"CRISPR-Cas in Streptococcus pyogenes","volume":"16","author":"Charpentier","year":"2019","journal-title":"RNA Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"513","DOI":"10.3389\/fphar.2019.00513","article-title":"Advantages and limitations of bacteriophages for the treatment of bacterial infections","volume":"10","author":"Principi","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1111\/j.1749-4486.2009.01973.x","article-title":"A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa: A preliminary report of efficacy","volume":"34","author":"Wright","year":"2009","journal-title":"Clin. Otolaryngol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1093\/emph\/eoy005","article-title":"Phage treatment of an aortic graft infected with Pseudomonas aeruginosa","volume":"2018","author":"Chan","year":"2018","journal-title":"Evol. Med. Public Health"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"69","DOI":"10.2174\/138920110790725401","article-title":"Phage Therapy in Clinical Practice: Treatment of Human Infections","volume":"11","author":"Kutter","year":"2010","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"G\u00f3rski, A., Mi\u0119dzybrodzki, R., \u0141obocka, M., G\u0142owacka-Rutkowska, A., Bednarek, A., Borysowski, J., Jo\u0144czyk-Matysiak, E., \u0141usiak-Szelachowska, M., Weber-D\u0105browska, B., and Bagi\u0144ska, N. (2018). Phage therapy: What have we learned?. Viruses, 10.","DOI":"10.3390\/v10060288"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"909","DOI":"10.2147\/DDDT.S189847","article-title":"Mechanistic research holds promise for bacterial vaccines and phage therapies for Pseudomonas aeruginosa","volume":"13","author":"Hoggarth","year":"2019","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1128\/AAC.00669-09","article-title":"Bacteriophage cocktail for the prevention of biofilm formation by Pseudomonas aeruginosa on catheters in an in vitro model system","volume":"54","author":"Fu","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Torres-Barcel\u00f3, C., Arias-S\u00e1nchez, F.I., Vasse, M., Ramsayer, J., Kaltz, O., and Hochberg, M.E. (2014). A window of opportunity to control the bacterial pathogen Pseudomonas aeruginosa combining antibiotics and phages. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0106628"},{"key":"ref_48","first-page":"1","article-title":"Emerging therapies against infections with Pseudomonas aeruginosa [version 1; peer review: 2 approved]","volume":"8","year":"2019","journal-title":"F1000Research"},{"key":"ref_49","first-page":"66","article-title":"Experimental phage therapy of burn wound infection: Difficult first steps","volume":"4","author":"Rose","year":"2014","journal-title":"Int. J. Burns Trauma."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Markwitz, P., Olszak, T., Gula, G., Kowalska, M., Arabski, M., and Drulis-Kawa, Z. (2021). Emerging Phage Resistance in Pseudomonas aeruginosa PAO1 Is Accompanied by an Enhanced Heterogeneity and Reduced Virulence. Viruses, 13.","DOI":"10.3390\/v13071332"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.jinf.2021.05.027","article-title":"Successful case of adjunctive intravenous bacteriophage therapy to treat left ventricular assist device infection","volume":"83","author":"Tkhilaishvili","year":"2021","journal-title":"J. Infect."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.rvsc.2021.04.010","article-title":"Efficacy assessment of PEV2 phage on Galleria mellonella larvae infected with a Pseudomonas aeruginosa dog otitis isolate","volume":"136","author":"Antoine","year":"2021","journal-title":"Res. Vet. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Aghaee, B.L., Khan Mirzaei, M., Alikhani, M.Y., Mojtahedi, A., and Maurice, C.F. (2021). Improving the Inhibitory Effect of Phages against Pseudomonas aeruginosa Isolated from a Burn Patient Using a Combination of Phages and Antibiotics. Viruses, 13.","DOI":"10.3390\/v13020334"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Krylov, V., Bourkaltseva, M., Pleteneva, E., Shaburova, O., Krylov, S., Karaulov, A., Zhavoronok, S., Svitich, O., and Zverev, V. (2021). Phage phikz\u2014The first of giants. Viruses, 13.","DOI":"10.3390\/v13020149"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"198250","DOI":"10.1016\/j.virusres.2020.198250","article-title":"A single dose of a virulent bacteriophage vB PaeP-SaPL, rescues bacteremic mice infected with multi drug resistant Pseudomonas aeruginosa","volume":"292","author":"Alvi","year":"2021","journal-title":"Virus. Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1097\/QCO.0000000000000677","article-title":"Current choices of antibiotic treatment for Pseudomonas aeruginosa infections","volume":"33","author":"Ibrahim","year":"2020","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_57","first-page":"1","article-title":"Pharmacokinetics and time-kill study of inhaled antipseudomonal bacteriophage therapy in mice","volume":"65","author":"Chow","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12941-020-00389-5","article-title":"Bacteriophage therapy against Pseudomonas aeruginosa biofilms: A review","volume":"19","author":"Chegini","year":"2020","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3278","DOI":"10.1111\/1462-2920.14310","article-title":"Metagenome analysis of Russian and Georgian Pyophage cocktails and a placebo-controlled safety trial of single phage versus phage cocktail in healthy Staphylococcus aureus carriers","volume":"20","author":"McCallin","year":"2018","journal-title":"Environ. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1111\/1348-0421.12846","article-title":"Susceptibility of Pseudomonas aeruginosa veterinary isolates to Pbunavirus PB1-like phages","volume":"64","author":"Fujiki","year":"2020","journal-title":"Microbiol. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1080\/17460441.2020.1803274","article-title":"Novel therapeutic strategies for treating Pseudomonas aeruginosa infection","volume":"15","author":"Shao","year":"2020","journal-title":"Expert Opin. Drug Discov."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1097\/QCO.0000000000000658","article-title":"Bacteriophage therapy as a treatment option for transplant infections","volume":"33","author":"Aslam","year":"2020","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.cmi.2020.04.039","article-title":"Pharmacokinetics\/pharmacodynamics of antipseudomonal bacteriophage therapy in rats: A proof-of-concept study","volume":"26","author":"Lin","year":"2020","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1073\/pnas.1913234117","article-title":"Controlled phage therapy by photothermal ablation of specific bacterial species using gold nanorods targeted by chimeric phages","volume":"117","author":"Peng","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Olszak, T., Danis-Wlodarczyk, K., Arabski, M., Gula, G., Maciejewska, B., Wasik, S., Lood, C., Higgins, G., Harvey, B.J., and Lavigne, R. (2019). Pseudomonas aeruginosa PA5oct Jumbo Phage Impacts Planktonic and Biofilm Population and Reduces Its Host Virulence. Viruses, 11.","DOI":"10.3390\/v11121089"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1080\/03009734.2019.1688433","article-title":"Pharmacological limitations of phage therapy","volume":"124","author":"Nilsson","year":"2019","journal-title":"Upsala J. Med. Sci."},{"key":"ref_67","first-page":"1","article-title":"Bacteriophages as Adjuvant to Antibiotics for the Treatment of Periprosthetic Joint Infection Caused by Multidrug-Resistant Pseudomonas aeruginosa","volume":"64","author":"Tkhilaishvili","year":"2020","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1164\/rccm.201904-0839LE","article-title":"Bacteriophage therapy of ventilator-associated pneumonia and empyema caused by Pseudomonas aeruginosa","volume":"200","author":"Maddocks","year":"2019","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1007\/s15010-019-01319-0","article-title":"Successful adjunctive use of bacteriophage therapy for treatment of multidrug-resistant Pseudomonas aeruginosa infection in a cystic fibrosis patient","volume":"47","author":"Law","year":"2019","journal-title":"Infection"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1177\/1534734619835115","article-title":"Bacteriophage Therapy of Chronic Nonhealing Wound: Clinical Study","volume":"18","author":"Gupta","year":"2019","journal-title":"Int. J. Low. Extrem. Wounds"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.micpath.2019.01.032","article-title":"Isolation and characterization of a bacteriophage and its potential to disrupt multi-drug resistant Pseudomonas aeruginosa biofilms","volume":"128","author":"Yuan","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1093\/infdis\/jiy678","article-title":"Bacteriophage Therapy Increases Complement-Mediated Lysis of Bacteria and Enhances Bacterial Clearance after Acute Lung Infection with Multidrug-Resistant Pseudomonas aeruginosa","volume":"219","author":"Elgaml","year":"2019","journal-title":"J. Infect. Dis."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S1473-3099(18)30482-1","article-title":"Efficacy and tolerability of a cocktail of bacteriophages to treat burn wounds infected by Pseudomonas aeruginosa (PhagoBurn): A randomised, controlled, double-blind phase 1\/2 trial","volume":"19","author":"Jault","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/S1473-3099(18)30502-4","article-title":"Phage therapy 2.0: Where do we stand?","volume":"19","author":"Breederveld","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AAC.02573-17","article-title":"Design of a broad-range bacteriophage cocktail that reduces Pseudomonas aeruginosa biofilms and treats acute infections in two animal models","volume":"62","author":"Forti","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1007\/s00705-018-3811-0","article-title":"Piperacillin and ceftazidime produce the strongest synergistic phage\u2013antibiotic effect in Pseudomonas aeruginosa","volume":"163","author":"Uchiyama","year":"2018","journal-title":"Arch. Virol."},{"key":"ref_77","first-page":"3612015","article-title":"In Vivo Studies on the Influence of Bacteriophage Preparations on the Autoimmune Inflammatory Process","volume":"2017","author":"Borysowski","year":"2017","journal-title":"BioMed Res. Int."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Villarroel, J., Larsen, M.V., Kilstrup, M., and Nielsen, M. (2017). Metagenomic Analysis of Therapeutic PYO Phage Cocktails from 1997 to 2014. Viruses, 9.","DOI":"10.3390\/v9110328"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"418","DOI":"10.3389\/fcimb.2017.00418","article-title":"Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients","volume":"7","author":"Fong","year":"2017","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"7977","DOI":"10.1007\/s00253-017-8504-1","article-title":"Immunogenicity and antimicrobial effectiveness of Pseudomonas aeruginosa specific bacteriophage in a human lung in vitro model","volume":"101","author":"Shiley","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.jcf.2017.06.012","article-title":"The potential of phage therapy in cystic fibrosis: Essential human-bacterial-phage interactions and delivery considerations for use in Pseudomonas aeruginosa-infected airways","volume":"16","author":"Trend","year":"2017","journal-title":"J. Cyst. Fibros."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.chom.2017.06.018","article-title":"Synergy between the host immune system and bacteriophage is essential for successful phage therapy against an acute respiratory pathogen","volume":"22","author":"Roach","year":"2017","journal-title":"Cell Host Microbe"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1136\/thoraxjnl-2016-209265","article-title":"Phage therapy is highly effective against chronic lung infections with Pseudomonas aeruginosa","volume":"72","author":"Waters","year":"2017","journal-title":"Thorax"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Al-Wrafy, F., Brzozowska, E., G\u00f3rska, S., and Gamian, A. (2017). Pathogenic factors of Pseudomonas aeruginosa-the role of biofilm in pathogenicity and as a target for phage therapy. Adv. Hyg. Exp. Med. Hig. Med. Dosw., 71.","DOI":"10.5604\/01.3001.0010.3792"},{"key":"ref_85","first-page":"668","article-title":"Phages, fitness, virulence, and synergy: A novel approach for the therapy of infections caused by Pseudomonas aeruginosa","volume":"215","author":"Stratton","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_86","first-page":"703","article-title":"Synergistic interaction between phage therapy and antibiotics clears Pseudomonas aeruginosa infection in endocarditis and reduces virulence","volume":"215","author":"Oechslin","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1073\/pnas.1617415113","article-title":"Quorum sensing controls the Pseudomonas aeruginosa CRISPR-Cas adaptive immune system","volume":"114","author":"Paczkowski","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"5332","DOI":"10.1128\/AEM.01166-16","article-title":"Phage therapy is effective in a mouse model of bacterial equine keratitis","volume":"82","author":"Furusawa","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_89","first-page":"1","article-title":"A proposed integrated approach for the preclinical evaluation of phage therapy in Pseudomonas infections","volume":"6","author":"Vandenheuvel","year":"2016","journal-title":"Sci Rep."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1002\/jobm.201600108","article-title":"Preliminary survey of local bacteriophages with lytic activity against multi-drug resistant bacteria","volume":"56","author":"Latz","year":"2016","journal-title":"J. Basic Microbiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1002\/jobm.201500764","article-title":"A virulent phage JHP against Pseudomonas aeruginosa showed infectivity against multiple genera","volume":"56","author":"Khawaja","year":"2016","journal-title":"J. Basic Microbiol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1039\/C5IB00267B","article-title":"Genetically modified bacteriophages","volume":"8","author":"Sagona","year":"2016","journal-title":"Integr. Biol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1038\/s41598-018-37636-x","article-title":"Phage therapy against Pseudomonas aeruginosa infections in a cystic fibrosis zebrafish model","volume":"9","author":"Cafora","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_94","unstructured":"Clokie, M.R.J., Kropinski, A., and Lavigne, R. (2019). Exploitation of Drosophila Infection Models to Evaluate Antibacterial Efficacy of Phages BT-Bacteriophages: Methods and Protocols, Springer."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.ijpharm.2019.01.050","article-title":"Storage stability of inhalable phage powders containing lactose at ambient conditions","volume":"560","author":"Chang","year":"2019","journal-title":"Int. J. Pharm."}],"container-title":["Microbiology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2036-7481\/13\/1\/2\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:54:44Z","timestamp":1760169284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2036-7481\/13\/1\/2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,28]]},"references-count":95,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["microbiolres13010002"],"URL":"https:\/\/doi.org\/10.3390\/microbiolres13010002","relation":{},"ISSN":["2036-7481"],"issn-type":[{"value":"2036-7481","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,28]]}}}