{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T10:58:53Z","timestamp":1768474733406,"version":"3.49.0"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T00:00:00Z","timestamp":1647388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["EXPL\/SAU-INF\/0261\/2021"],"award-info":[{"award-number":["EXPL\/SAU-INF\/0261\/2021"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Multidrug-resistant (MDR) Enterococcus faecium (Efm) infections continue to increase worldwide, although epidemiological studies remain scarce in lower middle-income countries. We aimed to explore which strains circulate in E. faecium causing human infections in Tunisian healthcare institutions in order to compare them with strains from non-human sources of the same country and finally to position them within the global E. faecium epidemiology by genomic analysis. Antibiotic susceptibility testing was performed and transfer of vancomycin-vanA and ampicillin-pbp5 resistance was performed by conjugation. WGS-Illumina was performed on Tunisian strains, and these genomes were compared with Efm genomes from other regions present in the GenBank\/NCBI database (n = 10,701 Efm genomes available May 2021). A comparison of phenotypes with those predicted by the recent ResFinder 4.1-CGE webtool unveiled a concordance of 88%, with discordant cases being discussed. cgMLST revealed three clusters [ST18\/CT222 (n = 13), ST17\/CT948 strains (n = 6), and ST203\/CT184 (n = 3)], including isolates from clinical, healthy-human, retail meat, and\/or environmental sources in different countries over large time spans (10\u201312 years). Isolates within each cluster showed similar antibiotic resistance, bacteriocin, and virulence genetic patterns. pbp5-AmpR was transferred by VanA-AmpR-ST80 (clinical) and AmpR-ST17-Efm (bovine meat). Identical chromosomal pbp5-platforms carrying metabolic\/virulence genes were identified between ST17\/ST18 strains of clinical, farm animal, and retail meat sources. The overall results emphasize the role of high-resolution genotyping as provided by WGS in depicting the dispersal of MDR-Efm strains carrying relevant adaptive traits across different hosts\/regions and the need of a One Health task force to curtail their spread.<\/jats:p>","DOI":"10.3390\/microorganisms10030632","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T22:15:04Z","timestamp":1647468904000},"page":"632","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["High-Resolution Genotyping Unveils Identical Ampicillin-Resistant Enterococcus faecium Strains in Different Sources and Countries: A One Health Approach"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7326-4133","authenticated-orcid":false,"given":"Ana R.","family":"Freitas","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, UCIBIO\u2014Applied Molecular Biosciences Unit, REQUIMTE, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"TOXRUN\u2014Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL, 4585-116 Gandra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1016-7173","authenticated-orcid":false,"given":"Ana P.","family":"Tedim","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Biom\u00e9dica en Sepsis-BioSepsis, Hospital Universitario R\u00edo Hortega, Instituto de Investigaci\u00f3n Biom\u00e9dica de Salamanca (IBSAL), 47012 Valladollid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6711-4780","authenticated-orcid":false,"given":"Ana C.","family":"Almeida-Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, UCIBIO\u2014Applied Molecular Biosciences Unit, REQUIMTE, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4239-1575","authenticated-orcid":false,"given":"B\u00e1rbara","family":"Duarte","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, UCIBIO\u2014Applied Molecular Biosciences Unit, REQUIMTE, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Houyem","family":"Elghaieb","sequence":"additional","affiliation":[{"name":"Tunisian Institute of Veterinary Research, University of Tunis El Manar, Tunis 1006, Tunisia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9673-193X","authenticated-orcid":false,"given":"Mohamed S.","family":"Abbassi","sequence":"additional","affiliation":[{"name":"Tunisian Institute of Veterinary Research, University of Tunis El Manar, Tunis 1006, Tunisia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0555-2974","authenticated-orcid":false,"given":"Abdennaceur","family":"Hassen","sequence":"additional","affiliation":[{"name":"Laboratory of Treatment and Valorisation of Wastewater, Centre of Research and Water Technologies (CERTE), Technopark of Borj-C\u00e9dria, Soliman 8020, Tunisia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3826-6731","authenticated-orcid":false,"given":"Carla","family":"Novais","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, UCIBIO\u2014Applied Molecular Biosciences Unit, REQUIMTE, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5810-8215","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Peixe","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, UCIBIO\u2014Applied Molecular Biosciences Unit, REQUIMTE, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"},{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.drup.2018.10.002","article-title":"Update on prevalence and mechanisms of resistance to linezolid, tigecycline and daptomycin in enterococci in Europe: Towards a common nomenclature","volume":"40","author":"Bender","year":"2018","journal-title":"Drug Resist. 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