{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:57:44Z","timestamp":1768517864677,"version":"3.49.0"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T00:00:00Z","timestamp":1658275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU Health Programme","award":["677 066"],"award-info":[{"award-number":["677 066"]}]},{"name":"EU Health Programme","award":["848096-SHARP JA"],"award-info":[{"award-number":["848096-SHARP JA"]}]},{"name":"Consumers, Health, Agriculture and Food Executive Agency (CHAFEA, European Commission)","award":["677 066"],"award-info":[{"award-number":["677 066"]}]},{"name":"Consumers, Health, Agriculture and Food Executive Agency (CHAFEA, European Commission)","award":["848096-SHARP JA"],"award-info":[{"award-number":["848096-SHARP JA"]}]},{"name":"Joint Action EMERGE","award":["677 066"],"award-info":[{"award-number":["677 066"]}]},{"name":"Joint Action EMERGE","award":["848096-SHARP JA"],"award-info":[{"award-number":["848096-SHARP JA"]}]},{"name":"Joint Action SHARP","award":["677 066"],"award-info":[{"award-number":["677 066"]}]},{"name":"Joint Action SHARP","award":["848096-SHARP JA"],"award-info":[{"award-number":["848096-SHARP JA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Brucellosis, mainly caused by Brucella (B.) melitensis, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for B. melitensis are not available, and the agent is not yet listed in the EUCAST breakpoint tables. CLSI recommendations for B. melitensis exist, but they do not fulfill the requirements of the ISO 20776 standard regarding the culture medium and the incubation conditions. Under the third EU Health Programme, laboratories specializing in the diagnostics of highly pathogenic bacteria in their respective countries formed a working group within a Joint Action aiming to develop a suitable method for the AST of B. melitensis. Under the supervision of EUCAST representatives, this working group adapted the CLSI M45 document to the ISO 20776 standard after testing and validation. These adaptations included the comparison of various culture media, culture conditions and AST methods. A Standard Operation Procedure was derived and an interlaboratory validation was performed in order to evaluate the method. The results showed pros and cons for both of the two methods but also indicate that it is not necessary to abandon Mueller\u2013Hinton without additives for the AST of B. melitensis.<\/jats:p>","DOI":"10.3390\/microorganisms10071470","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T03:34:40Z","timestamp":1658374480000},"page":"1470","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6066-8246","authenticated-orcid":false,"given":"Alina","family":"Tscherne","sequence":"first","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany"},{"name":"Division of Virology, Department of Veterinary Sciences, LMU Munich, Veterin\u00e4rstrasse 13, 80539 Munich, Germany"}]},{"given":"Enrico","family":"Mantel","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany"}]},{"given":"Talar","family":"Boskani","sequence":"additional","affiliation":[{"name":"Public Health Agency of Sweden, Nobels v\u00e4g 18, 171 82 Solna, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2950-6078","authenticated-orcid":false,"given":"Sylwia","family":"Budniak","sequence":"additional","affiliation":[{"name":"National Veterinary Research Institute, 57 Partyzant\u00f3w Avenue, 24-100 Pulawy, Poland"}]},{"given":"Mandy","family":"Elschner","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany"}]},{"given":"Antonio","family":"Fasanella","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, Italy"}]},{"given":"Siri L.","family":"Feruglio","sequence":"additional","affiliation":[{"name":"Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1159-2801","authenticated-orcid":false,"given":"Domenico","family":"Galante","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, Italy"}]},{"given":"Christian G.","family":"Giske","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Alfred Nobels All\u00e9 8, Huddinge, 141 52 Stockholm, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8966-8469","authenticated-orcid":false,"given":"Roland","family":"Grunow","sequence":"additional","affiliation":[{"name":"Highly Pathogenic 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Germany"}]},{"given":"Agnieszka","family":"Kedrak-Jablonska","sequence":"additional","affiliation":[{"name":"National Veterinary Research Institute, 57 Partyzant\u00f3w Avenue, 24-100 Pulawy, Poland"}]},{"given":"Veronica K.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway"}]},{"given":"Tone B.","family":"Johansen","sequence":"additional","affiliation":[{"name":"Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway"}]},{"given":"Gunnar","family":"Kahlmeter","sequence":"additional","affiliation":[{"name":"EUCAST Development Laboratory (EDL) for Bacteria, 351 85 V\u00e4xj\u00f6, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8342-5375","authenticated-orcid":false,"given":"Viviana","family":"Manzulli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 20 Manfredonia Street, 72121 Foggia, Italy"}]},{"given":"Erika","family":"Matuschek","sequence":"additional","affiliation":[{"name":"EUCAST Development Laboratory (EDL) for Bacteria, 351 85 V\u00e4xj\u00f6, Sweden"}]},{"given":"Falk","family":"Melzer","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5182-6150","authenticated-orcid":false,"given":"Maria S.","family":"Nuncio","sequence":"additional","affiliation":[{"name":"National Institute of Health, Av. 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