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In this work, an isolation effort inspired by the iChip was performed using marine sediments from Memoria beach, Portugal. The isolates obtained were identified by 16S rRNA gene analysis, fingerprinted using BOX-PCR and ERIC-PCR, searched for the putative presence of secondary metabolism genes associated with polyketide synthase I (PKS-I) and non-ribosomal peptide synthetases (NRPS), screened for antimicrobial activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213, and had bioactive extracts dereplicated by LC\/HRMS. Of the 158 isolated strains, 96 were affiliated with the phylum Actinomycetota, PKS-I and NRPS genes were detected in 53 actinomycetotal strains, and 11 proved to be bioactive (10 against E. coli, 1 against S. aureus and 1 against both pathogens). Further bioactivities were explored using an \u201cone strain many compounds\u201d approach, with six strains showing continued bioactivity and one showing a novel one. Extract dereplication showed the presence of several known bioactive molecules and potential novel ones in the bioactive extracts. These results indicate the use of the bacteria isolated here as sources of new bioactive natural products.<\/jats:p>","DOI":"10.3390\/microorganisms10071471","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T03:34:40Z","timestamp":1658374480000},"page":"1471","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from Portuguese Beach Sediments"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9693-4857","authenticated-orcid":false,"given":"Jos\u00e9 Diogo Neves","family":"dos Santos","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre S\/N, 4169-007 Porto, Portugal"},{"name":"Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Susana Afonso","family":"Jo\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre S\/N, 4169-007 Porto, Portugal"},{"name":"Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Jes\u00fas","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avenida del Conocimiento, 34 Parque Tecnol\u00f3gico de Ciencias de la Salud, 18016 Granada, Spain"}]},{"given":"Francisca","family":"Vicente","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avenida del Conocimiento, 34 Parque Tecnol\u00f3gico de Ciencias de la Salud, 18016 Granada, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1607-5106","authenticated-orcid":false,"given":"Fernando","family":"Reyes","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda, Avenida del Conocimiento, 34 Parque Tecnol\u00f3gico de Ciencias de la Salud, 18016 Granada, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-3970","authenticated-orcid":false,"given":"Olga Maria","family":"Lage","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre S\/N, 4169-007 Porto, Portugal"},{"name":"Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,20]]},"reference":[{"key":"ref_1","unstructured":"NOAA (2020). 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