{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T21:21:51Z","timestamp":1774041711618,"version":"3.50.1"},"reference-count":81,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the European Regional Development Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Antimicrobial resistance has become a global problem in recent decades. A gradual reduction in drug discoveries has led to the current antimicrobial resistance crisis. Caves and other subsurface environments are underexplored thus far, and they represent indispensable ecological niches that could offer new molecules of interest to medicine and biotechnology. We explored Spanish show caves to test the bioactivity of the bacteria dwelling in the walls and ceilings, as well as airborne bacteria. We reported the isolation of two strains of the genus Crossiella, likely representing a new species, isolated from Altamira Cave, Spain. In vitro and in silico analyses showed the inhibition of pathogenic Gram-positive and Gram-negative bacteria, and fungi, as well as the taxonomical distance of both strains from their closest relative, Crossiella cryophila. The presence of an exclusive combination of gene clusters involved in the synthesis of lanthipeptides, lasso peptides, nonribosomal peptides and polyketides indicates that species of this genus could represent a source of new compounds. Overall, there is promising evidence for antimicrobial discovery in subterranean environments, which increases the possibility of identifying new bioactive molecules.<\/jats:p>","DOI":"10.3390\/microorganisms10081575","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T22:33:11Z","timestamp":1659997991000},"page":"1575","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["The Rare Actinobacterium Crossiella sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9687-3134","authenticated-orcid":false,"given":"Jose Luis","family":"Gonzalez-Pimentel","sequence":"first","affiliation":[{"name":"Laboratorio Hercules, Universidade de Evora, 7004-516 Evora, Portugal"}]},{"given":"Irene","family":"Dominguez-Mo\u00f1ino","sequence":"additional","affiliation":[{"name":"Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0972-9909","authenticated-orcid":false,"given":"Valme","family":"Jurado","sequence":"additional","affiliation":[{"name":"Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, Spain"}]},{"given":"Leonila","family":"Laiz","sequence":"additional","affiliation":[{"name":"Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5409-6990","authenticated-orcid":false,"given":"Ana Teresa","family":"Caldeira","sequence":"additional","affiliation":[{"name":"Laboratorio Hercules, Universidade de Evora, 7004-516 Evora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0036-670X","authenticated-orcid":false,"given":"Cesareo","family":"Saiz-Jimenez","sequence":"additional","affiliation":[{"name":"Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3181\/00379727-55-14461","article-title":"Streptomycin, a substance exhibiting antibiotic activity against gram-positive and gram-negative bacteria","volume":"55","author":"Schatz","year":"1944","journal-title":"Exp. 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