{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:09:22Z","timestamp":1777540162118,"version":"3.51.4"},"reference-count":71,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T00:00:00Z","timestamp":1670630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"AgriWWater Project","doi-asserted-by":"publisher","award":["PTDC\/CTA-AMB\/29586\/2017"],"award-info":[{"award-number":["PTDC\/CTA-AMB\/29586\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"AgriWWater Project","doi-asserted-by":"publisher","award":["UID\/Multi\/04462\/2019"],"award-info":[{"award-number":["UID\/Multi\/04462\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"AgriWWater Project","doi-asserted-by":"publisher","award":["UID\/Multi\/04413\/2013"],"award-info":[{"award-number":["UID\/Multi\/04413\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"iNOVA4Health","award":["PTDC\/CTA-AMB\/29586\/2017"],"award-info":[{"award-number":["PTDC\/CTA-AMB\/29586\/2017"]}]},{"name":"iNOVA4Health","award":["UID\/Multi\/04462\/2019"],"award-info":[{"award-number":["UID\/Multi\/04462\/2019"]}]},{"name":"iNOVA4Health","award":["UID\/Multi\/04413\/2013"],"award-info":[{"award-number":["UID\/Multi\/04413\/2013"]}]},{"name":"Global Health and Tropical Medicine Center","award":["PTDC\/CTA-AMB\/29586\/2017"],"award-info":[{"award-number":["PTDC\/CTA-AMB\/29586\/2017"]}]},{"name":"Global Health and Tropical Medicine Center","award":["UID\/Multi\/04462\/2019"],"award-info":[{"award-number":["UID\/Multi\/04462\/2019"]}]},{"name":"Global Health and Tropical Medicine Center","award":["UID\/Multi\/04413\/2013"],"award-info":[{"award-number":["UID\/Multi\/04413\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>In face of the absence of epidemiological data regarding the circulation of human adenoviruses (HAdV) in Portugal, this study aimed at the evaluation of their molecular diversity in waste and environmental waters in the Lisbon Metropolitan Area (LMA). Using samples collected between 2018 and 2021, the HAdV hexon protein-coding sequence was partially amplified using three nested touch-down PCR protocols. The amplification products obtained were analyzed in parallel by two approaches: molecular cloning followed by Sanger sequencing and Next-Generation Sequencing (NGS) using Illumina\u00ae sequencing. The analysis of NGS-generated data allowed the identification of a higher diversity of HAdV-A (19%), -B (1%), -C (3%), -D (24%), and -F (25%) viral types, along with murine adenovirus (MAdV-2; 30%) in the wastewater treatment plant samples. On the other hand, HAdV-A (19%), -D (32%), and -F (36%) were identified in environmental samples, and possibly MAdV-2 (14%). These results demonstrate the presence of fecal contamination in environmental waters and the assessment of the diversity of this virus provides important information regarding the distribution of HAdV in LMA, including the detection of HAdV-F41, the most frequently reported in water worldwide.<\/jats:p>","DOI":"10.3390\/microorganisms10122443","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T03:02:37Z","timestamp":1670814157000},"page":"2443","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9788-3668","authenticated-orcid":false,"given":"Joana","family":"Cavadas","sequence":"first","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica (iBET), Apartado 12, 2781-901 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4832-9898","authenticated-orcid":false,"given":"Ricardo","family":"Parreira","sequence":"additional","affiliation":[{"name":"Unidade de Microbiologia M\u00e9dica, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (NOVA), Rua da Junqueira No. 100, 1349-008 Lisboa, Portugal"},{"name":"Global Health and Tropical Medicine (GHTM) Research Centre, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8516-2487","authenticated-orcid":false,"given":"In\u00eas","family":"Leonardo","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica (iBET), Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier (ITQB), Universidade Nova de Lisboa (NOVA), Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5605-1419","authenticated-orcid":false,"given":"Maria Teresa","family":"Barreto Crespo","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica (iBET), Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier (ITQB), Universidade Nova de Lisboa (NOVA), Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5034-5494","authenticated-orcid":false,"given":"M\u00f3nica","family":"Nunes","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica (iBET), Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier (ITQB), Universidade Nova de Lisboa (NOVA), Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/j.scitotenv.2018.09.295","article-title":"Human Adenoviruses as Waterborne Index Pathogens and Their Use for Quantitative Microbial Risk Assessment","volume":"651","author":"Verani","year":"2019","journal-title":"Sci. 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