{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:01:39Z","timestamp":1760148099532,"version":"build-2065373602"},"reference-count":78,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior (FCT\/MCTES, Portugal)","doi-asserted-by":"publisher","award":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"],"award-info":[{"award-number":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"iNOVA4Health","award":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"],"award-info":[{"award-number":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"]}]},{"name":"Associate Laboratory LS4FUTURE","award":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"],"award-info":[{"award-number":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"]}]},{"name":"FCT\/MCTES","award":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"],"award-info":[{"award-number":["PTDC\/BAA-BIO\/1262\/2020","UIDB\/04462\/2020","UIDP\/04462\/2020","LA\/P\/0087\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Marine bacteria are a significant source of bioactive compounds for various biotechnological applications. Among these, actinomycetes have been found to produce a wide range of secondary metabolites of interest. Saccharopolyspora is one of the genera of actinomycetes that has been recognized as a potential source of these compounds. This study reports the characterization and genomic analysis of Saccharopolyspora sp. NFXS83, a marine bacterium isolated from seawater from the Sado estuary in Portugal. The NFXS83 strain produced multiple functional and stable extracellular enzymes under high-salt conditions, showed the ability to synthesize auxins such as indole-3-acetic acid, and produced diffusible secondary metabolites capable of inhibiting the growth of Staphylococcus aureus. Furthermore, when Phaeodactylum tricornutum was co-cultivated with strain NFXS83 a significant increase in microalgae cell count, cell size, auto-fluorescence, and fucoxanthin content was observed. Detailed analysis revealed the presence of clusters involved in the production of various secondary metabolites, including extracellular enzymes, antimicrobial compounds, terpenes, and carotenoids in the genome of strain NFXS83. Ultimately, these findings indicate that Saccharopolyspora sp. NFXS83 has a significant potential for a wide range of marine biotechnological applications.<\/jats:p>","DOI":"10.3390\/microorganisms11040902","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T03:02:01Z","timestamp":1680231721000},"page":"902","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Saccharopolyspora sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8731-6801","authenticated-orcid":false,"given":"Constan\u00e7a D. F.","family":"Bertrand","sequence":"first","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2781-901 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8248-2407","authenticated-orcid":false,"given":"Rodrigo","family":"Martins","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2781-901 Oeiras, Portugal"}]},{"given":"Francisco","family":"Quintas-Nunes","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2781-901 Oeiras, Portugal"},{"name":"ITQB\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0817-609X","authenticated-orcid":false,"given":"Pedro","family":"Reynolds-Brand\u00e3o","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2781-901 Oeiras, Portugal"}]},{"given":"Maria T. B.","family":"Crespo","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2781-901 Oeiras, Portugal"},{"name":"ITQB\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1184-3794","authenticated-orcid":false,"given":"Francisco X.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2781-901 Oeiras, Portugal"},{"name":"ITQB\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3354\/meps010257","article-title":"The ecological role of water-column microbes","volume":"10","author":"Azam","year":"1983","journal-title":"Mar. 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