{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T20:06:00Z","timestamp":1773691560107,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Algerian Ministry of High Education and Scientific Research, Algeria for the fellowship funding","award":["PNE\/299"],"award-info":[{"award-number":["PNE\/299"]}]},{"name":"Algerian Ministry of High Education and Scientific Research, Algeria for the fellowship funding","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"Algerian Ministry of High Education and Scientific Research, Algeria for the fellowship funding","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"Algerian Ministry of High Education and Scientific Research, Algeria for the fellowship funding","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]},{"name":"CIIMAR acknowledges the projects FCT","award":["PNE\/299"],"award-info":[{"award-number":["PNE\/299"]}]},{"name":"CIIMAR acknowledges the projects FCT","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"CIIMAR acknowledges the projects FCT","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"CIIMAR acknowledges the projects FCT","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]},{"name":"WP9-Portuguese Blue Biobank under the Blue Economy Pact","award":["PNE\/299"],"award-info":[{"award-number":["PNE\/299"]}]},{"name":"WP9-Portuguese Blue Biobank under the Blue Economy Pact","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"WP9-Portuguese Blue Biobank under the Blue Economy Pact","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"WP9-Portuguese Blue Biobank under the Blue Economy Pact","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Monitoring water supply requires, among other quality indicators, the identification of the cyanobacteria community and taking into account their potential impact in terms of water quality. In this work, cyanobacteria strains were isolated from the Cheffia Reservoir and identified based on morphological features, the 16S rRNA gene, phylogenetic analysis, and toxin production by polymerase chain reaction PCR screening of the genes involved in the biosynthesis of cyanotoxins (mcyA, mcyE, sxtA, sxtG, sxtI, cyrJ, and anaC). Thirteen strains representing six different genera: Aphanothece, Microcystis, Geitlerinema, Lyngbya, Microcoleus, and Pseudanabaena were obtained. The results demonstrated the importance of morphological features in determining the genus or the species when incongruence between the morphological and phylogenetic analysis occurs and only the utility of the 16S rRNA gene in determining higher taxonomic levels. The phylogenetic analysis confirmed the polyphyly of cyanobacteria for the Microcystis and Oscillatoriales genera. Unexpectedly, Aphanothece sp. CR 11 had the genetic potential to produce microcystins. Our study gives new insight into species with picoplanktonic (or small) cell size and potentially toxic genotypes in this ecosystem. Thus, conventional water treatment methods in this ecosystem have to be adapted, indicating the requirement for pre-treatment methods that can effectively eliminate picocyanobacteria while preserving cell integrity to prevent toxin release.<\/jats:p>","DOI":"10.3390\/microorganisms11112664","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T12:50:42Z","timestamp":1698670242000},"page":"2664","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["First Polyphasic Study of Cheffia Reservoir (Algeria) Cyanobacteria Isolates Reveals Toxic Picocyanobacteria Genotype"],"prefix":"10.3390","volume":"11","author":[{"given":"Lamia","family":"Benredjem","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology, Faculty of Natural and Life Sciences, Abbes Laghrour University of Khenchela, BP 1252 Road of Batna, Khenchela 40004, Algeria"},{"name":"Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, BP 12, Annaba 23000, Algeria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4190-0959","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Morais","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leix\u00f5es, University of Porto, 4450-208 Matosinhos, Portugal"},{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4396-024X","authenticated-orcid":false,"given":"Guilherme Scotta","family":"Hentschke","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leix\u00f5es, University of Porto, 4450-208 Matosinhos, Portugal"}]},{"given":"Akila","family":"Abdi","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, BP 12, Annaba 23000, Algeria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9690-778X","authenticated-orcid":false,"given":"Hajira","family":"Berredjem","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University, BP 12, Annaba 23000, Algeria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, Terminal de Cruzeiros do Porto de Leix\u00f5es, University of Porto, 4450-208 Matosinhos, Portugal"},{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, Edif\u00edcio FC4, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.1007\/s00204-022-03354-7","article-title":"A Review and assessment of cyanobacterial toxins as cardiovascular health hazards","volume":"96","author":"Chen","year":"2022","journal-title":"Arch. 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