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Its occurrence in bats is poorly described and may impact the disease\u2019s maintenance and epidemiology. The aim of this study was to detect the agent by PCR assays targeting kDNA and nuclear DNA in the organs of 203 urban bats and rural vampire bats from the Brazilian Atlantic Forest, S\u00e3o Paulo state, during the pandemic period from 2020 to 2022. In total, 6 of the 203 bats (2.97%) were positive for T. cruzi. Infection was detected in 2% (2\/101) of Desmodus rotundus, 33% (1\/3) of Nyctinomops laticaudatus, 25% (1\/4) of Artibeus lituratus, 4% (1\/24) of Eumops glaucinus and in 2% (1\/41) of Molossus molossus. The gene sequences obtained were assessed for quality and deposited in a public repository. Fruit bats were statistically associated with positivity for T. cruzi. To our knowledge, this study detected T. cruzi for the first time in bats from S\u00e3o Paulo state and in N. laticaudatus and E. glaucinus species.<\/jats:p>","DOI":"10.3390\/microorganisms12050945","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T06:53:27Z","timestamp":1715064807000},"page":"945","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Trypanosoma cruzi in Bats (Chiroptera; Mammalia) from the Brazilian Atlantic Forest, S\u00e3o Paulo State"],"prefix":"10.3390","volume":"12","author":[{"given":"Danilo Alves de","family":"Fran\u00e7a","sequence":"first","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-681, SP, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5668-745X","authenticated-orcid":false,"given":"Mariana","family":"Louro","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Faculty of Veterinary Medicine, 1300-477 Lisbon, Portugal"}]},{"given":"Sara","family":"Z\u00faquete","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Faculty of Veterinary Medicine, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5457-5016","authenticated-orcid":false,"given":"Dayane da Silva","family":"Zanini","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-681, SP, Brazil"}]},{"given":"Gustavo Nunes de","family":"Moraes","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-681, SP, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8052-7109","authenticated-orcid":false,"given":"Gabrielle dos Santos","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-681, SP, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7554-1520","authenticated-orcid":false,"given":"Leandro Meneguelli","family":"Biondo","sequence":"additional","affiliation":[{"name":"National Institute of the Atlantic Forest (INMA), Brazilian Ministry of Science, Technology and Innovation, Santa Teresa 29650-000, ES, Brazil"},{"name":"Interdisciplinary Graduate Studies, University of British Columbia, Kelowna, BC V1V 1V7, Canada"}]},{"given":"Felipe","family":"Fornazari","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-681, SP, Brazil"}]},{"given":"Benedito Donizete","family":"Menozzi","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-681, SP, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6632-1224","authenticated-orcid":false,"given":"Isabel Pereira da","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Faculty of Veterinary Medicine, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-0762","authenticated-orcid":false,"given":"Helio","family":"Langoni","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-681, SP, 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